Virg_00270 Virg_00270   Virg_00290 Virg_00290

Virg_00280 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction95,484 / 94,867 / - [in whole cluster]
737 / 120 / - [in contig]
Locationcomplement(94867..95484) [in whole cluster]
complement(120..737) [in contig]
TypeCDS
Length618 bp (205 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative TetR family transcriptional regulator
Product (GenBank)BarZ
Gene
Gene (GenBank)barZ
EC number
Keyword
Note
  • This ORF is involved in both virginiamycin M and virginiamycin S biosynthesis.
Note (GenBank)
  • BarB homolog
Reference
ACC
PmId
[10792718] Identification of an AfsA homologue (BarX) from Streptomyces virginiae as a pleiotropic regulator controlling autoregulator biosynthesis, virginiamycin biosynthesis and virginiamycin M1 resistance. (Mol Microbiol. , 2000)
[14647980] Identification by gene deletion analysis of barB as a negative regulator controlling an early process of virginiamycin biosynthesis in Streptomyces virginiae. (Arch Microbiol. , 2004)
comment
[PMID:10792718]
barXの機能解析論文。

barX欠損株における転写制御の変化を見ている。
barZ,orf2,varM,orf4,orf5は、barA,barBのregulator proteinによって制御される。

[PMID:14647980(2004)]
barBの機能解析論文。

barB欠損株作製、BarBはdirectly or indirectlyにbarZ, vmsR, and orf5の転写をnegative制御する。
barB破壊株におけるvriginiamycin Mとvriginiamycin Sの生産の変化をみているfig.あり。


続報(和文)にて、抑制性制御遺伝子barB,barZ破壊株におけるvriginiamycin Mとvriginiamycin Sの生産の変化をみている。
BarBはvirginiamycin 生産の初期過程を，またBarZは後期過程を抑制していることが判明し，特にbarZ遺伝子の破壊によりvirginiamycinの生産量を顕著に増加させることが可能となった(unpublished)との記述あり。
Family/Domain
FD
IPR001647
comment
[IPR001647] Transcription regulator, TetR-like, DNA-binding, bacterial/archaeal (Domain)

close this sectionSequence

selected fasta
>putative TetR family transcriptional regulator [BarZ]
MQQRSVITCRRLVTAAAELFDRKGYVNATLGEITGAARVTKGALYFHFASKGELAHAVEE
HGCTLLRPVGRPARRRQQLTAADPDRLHPLAGPGAARGSRAPRNFRLSRELRGGEAVGGG
FTEAWMTTAWSLLDRAGAAGELRNGADRAGTADAARPRRWPASNCSLPPTCRTRNSPPGS
LPCGTSLCPGLVGPSGRTDYRTTPR
selected fasta
>putative TetR family transcriptional regulator [BarZ]
ATGCAGCAGAGATCGGTGATCACCTGTCGGAGGCTGGTGACCGCTGCAGCGGAACTGTTC
GACCGCAAGGGGTACGTGAACGCGACCCTGGGGGAGATCACCGGTGCGGCACGGGTCACC
AAGGGGGCCCTGTACTTCCACTTCGCCTCCAAGGGCGAACTGGCCCACGCCGTGGAGGAG
CACGGCTGCACCCTGCTTCGCCCAGTCGGTCGACCGGCTCGCCGCCGGCAGCAGCTCACC
GCTGCAGACCCTGATCGACTTCACCCACTGGCTGGCCCGGGGGCTGCGCGAGGAAGCCGC
GCTCCGCGCAACTTCCGCCTCAGCAGGGAACTGCGCGGCGGGGAGGCGGTCGGCGGCGGC
TTCACCGAGGCGTGGATGACCACCGCCTGGTCGCTGCTCGACCGGGCCGGTGCGGCGGGG
GAACTGCGCAACGGCGCCGACCGCGCGGGAACCGCAGACGCTGCTCGCCCGCGGCGGTGG
CCGGCATCGAACTGCTCGCTGCCACCGACATGTCGGACGAGGAACTCACCGCCCGGGTCG
CTGCCCTGTGGGACCTCGCTCTGCCCCGGCCTGGTCGGGCCGTCCGGACGGACCGACTAT
CGGACGACTCCCCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001647 DNA-binding HTH domain, TetR-type (Domain)
[6-66] PS50977
PS50977   HTH_TETR_2
[12-25] 9.00000407957274e-10 PR00455 [33-56] 9.00000407957274e-10 PR00455
PR00455   HTHTETR
[12-58] 2.4e-14 PF00440
PF00440   TetR_N
IPR009057 Homeodomain-like (Domain)
[1-76] 6.09999977126211e-15 SSF46689
SSF46689   Homeodomain_like
IPR015893 Tetracycline transcriptional regulator, TetR-like, C-terminal (Domain)
[1-154] 1.4e-20 G3DSA:1.10.357.10
G3DSA:1.10.357.10   TetR_C
IPR023772 DNA-binding HTH domain, TetR-type, conserved site (Conserved_site)
[24-54] PS01081
PS01081   HTH_TETR_1
SignalP
[1-17] 0.343 Signal
Eukaryota   
TMHMM No significant hit
Virg_00270 Virg_00270   Virg_00290 Virg_00290
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