Virg_00340 Virg_00340   Virg_00360 Virg_00360

Virg_00350 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction103,614 / 104,264 / + [in whole cluster]
373 / 1,023 / + [in contig]
Location103614..104264 [in whole cluster]
373..1023 [in contig]
TypeCDS
Length651 bp (216 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative TetR family transcriptional regulator
Product (GenBank)BarB
Gene
Gene (GenBank)barB
EC number
Keyword
Note
  • This ORF is involved in both virginiamycin M and virginiamycin S biosynthesis.
Note (GenBank)
Reference
ACC
PmId
[14647980] Identification by gene deletion analysis of barB as a negative regulator controlling an early process of virginiamycin biosynthesis in Streptomyces virginiae. (Arch Microbiol. , 2004)
comment
barB機能解析の報告

barB欠損株作製し、機能同定、virginiae butanolide-BarA signaling cascadeに関与する遺伝子の発現の変化を見ている。

virginiamycin生合成のvirginiamycin 生産の初期過程で働くnegative regulatorだと推定している。

barB欠損株は、細胞分化とvirginiae butanolide生合成どちらにも関与しない。
また、barB欠損株においてvirginiamycin M1とvirginiamycin Sの生産はvirginiae butanolide生産後に行われ、WTよりも2-3h早かったことから、virginiae butanolideがBarAのrepressor機能を不活性化した後に、BarBはvirginiamycin生産の経時的な遅延に関与すると示唆。
RT-PCRの解析結果から、barBの転写はbarAにより抑制され、barBは上流のbarZ, vmsR, orf5の転写をdirectly or indirectlyにnegative制御すると考察。

close this sectionSequence

selected fasta
>putative TetR family transcriptional regulator [BarB]
MTPKQERAFRTRTQLVLSAAEAFDRQGFATASLTAISNSAGVSNGALHFHFESKEALAAA
VEAEAAERMRTIVDGAARRGASALQALVDTSHAVMLRLRQDVVVRAGFRLSGDAARQATH
DLPEHWRQSVVRLLERAGRDGSLTSAVTPSDVAGVVTATVLGFGVLARFDSAWLASGSLS
GFWKLMLPMIAAGPVERGELDCRPAVPADVRRAPAV
selected fasta
>putative TetR family transcriptional regulator [BarB]
TTGACACCCAAACAGGAACGCGCCTTCCGCACCCGCACCCAGCTGGTGCTCTCGGCGGCC
GAGGCCTTCGATCGGCAGGGTTTCGCGACGGCCTCGCTCACCGCCATCAGCAACAGCGCC
GGTGTCAGTAACGGGGCCCTGCACTTCCACTTCGAGAGCAAGGAAGCGCTCGCCGCCGCC
GTCGAAGCGGAGGCGGCCGAGCGGATGCGGACGATCGTCGACGGAGCGGCCCGGAGGGGC
GCTTCCGCGCTCCAGGCCCTGGTCGACACCAGTCACGCCGTCATGCTCCGGCTGCGCCAG
GACGTGGTGGTGCGCGCCGGGTTCCGGCTCAGCGGCGATGCGGCCCGACAGGCCACCCAC
GACCTCCCGGAGCACTGGCGCCAGAGTGTCGTGCGGCTGCTGGAGCGGGCCGGCCGGGAC
GGCTCGCTGACCTCGGCCGTGACGCCCTCGGACGTCGCCGGGGTCGTGACGGCCACCGTC
CTCGGATTCGGCGTGCTGGCGAGGTTCGACTCCGCCTGGCTGGCCTCCGGATCCCTCTCC
GGGTTCTGGAAGCTGATGCTCCCCATGATCGCGGCCGGTCCGGTCGAGCGCGGCGAACTG
GACTGCCGGCCCGCCGTGCCGGCCGACGTCCGGCGCGCGCCCGCCGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001647 DNA-binding HTH domain, TetR-type (Domain)
[15-28] 6.80000200614765e-08 PR00455 [36-59] 6.80000200614765e-08 PR00455
PR00455   HTHTETR
[17-61] 1.4e-12 PF00440
PF00440   TetR_N
[9-69] PS50977
PS50977   HTH_TETR_2
IPR009057 Homeodomain-like (Domain)
[1-87] 2.2999993566538e-15 SSF46689
SSF46689   Homeodomain_like
IPR011075 Tetracycline transcriptional regulator, TetR-related, C-terminal (Domain)
[81-194] 1.89999859865865e-21 SSF48498
SSF48498   TetR_like_C
IPR015893 Tetracycline transcriptional regulator, TetR-like, C-terminal (Domain)
[10-194] 9e-37 G3DSA:1.10.357.10
G3DSA:1.10.357.10   TetR_C
IPR023772 DNA-binding HTH domain, TetR-type, conserved site (Conserved_site)
[27-57] PS01081
PS01081   HTH_TETR_1
SignalP No significant hit
TMHMM No significant hit
Virg_00340 Virg_00340   Virg_00360 Virg_00360
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