Azino_00030 Azino_00030   Azino_00050 Azino_00050

Azino_00040 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction10,513 / 7,202 / - [in whole cluster]
10,513 / 7,202 / - [in contig]
Locationcomplement(7202..10513) [in whole cluster]
complement(7202..10513) [in contig]
TypeCDS
Length3,312 bp (1,103 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)Azi3
GeneaziA4
Gene (GenBank)azi3
EC number
Keyword
Note
Note (GenBank)
  • NRPS(C-A-PCP)
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
comment
Azinomycin B生合成遺伝子クラスターの報告。
aziA1-A5はmodular NRPS genesで、推定産物は非定型的なNRPS systemを構成する。

aziA4: NRPS (C-A-PCP)

AziA4は典型的なNRPSの構成をとる。
AziA4-Aの基質特異性を与えるコードはDVFDFGGVCK(unusual substrate)と同定。AziA4のA domainは、基質としてaziridino[1, 2a]pyrrolidinyl amino acidを認識するため新規であるに違いないと主張している。

close this sectionPKS/NRPS Module

B4 aziridino[1, 2a]pyrrolidinyl amino acid
C35..331
A515..908
PCP991..1062

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [Azi3]
MSQQSELLRRLRRLPGERRDALLAQLEDVSAGARSAPLSYRQEQLWLFDRFAPSSLAYGL
GFALPIEGRLDTEALGAALADLLQRHTVLRSTFSDEDTRGTQRLKPVPRIRLETEDVPAE
RLHPRADEVVQGELRRGFDLNSGPMVRFRLLRSAPDLHLLVLTSHSIVLDPRSARVLQGD
LAHAYRARAAGAAPAWAQDPVEFGTYAAWQRAWCEQDAAREATTYWRDALAGWESTELPT
DLPRPRLIDLSSDVVRRPLPPGLAAAADDLATGSGAERRDVLLAAFLVVIARHTSRGDLT
IGLPQDVAGPYDESRLVGDIGNLLPLRVELDEADSFAAVVARVRDARRDGLHHASLPFKL
ILDALGVEPDAGRLPLVQIGFTTPVAGGGPAEADGVRFGCEQIDAGSGAFELALEVTDGE
QPTVAVRFATSLYRRATATRVLDRFLQTLDALCRQPRRPLGDAPLGTPQEHADVRERWNE
PIGAPADTPLHDLFAEVVAAAPDNTALLWKGGSLSYGELDAWSWRIARRLTAAGVGLGDL
VPVVMQRGPALLAAVLGVLRAGGAYVPVDAGQPDERLAMILEDTGARTVVVSPGTADRVP
GPLALVVGADPLDGPQAEEEPPAVVVPAASAAYVIYTSGSTGRPKGVVVEHRNAANFTRT
VRDMFDLTPEDRVLHFASPGFDVSVFEIFGTLLSGAVLYVLDEDQRRSVDALDAVLQEQR
ITVIDLPPAIMDLLAPENYPDLRVAFVGGEAFTGELTTRWARGRSFWNGYGPTETTVTVV
AKRCEGEWTASPPIGRAMANHRAYVVDGAGASLLPAGAVGELAIAGLGVARGYLGQPALT
ADRFRPDPDGPPGSRRYLTGDLAVWDEAGDLCFVGRADRQVKVRGVRIELGEVEAALQAV
DGVAQAVAEVATDPRTGALLVAYVVPERPGELQLDTIRSAVARRLPAAMVPSVLVPLDAV
PLTPSGKIDRRRLPAVEFAVVDELSEADEENSTPTERTMREEVFTPLLGARIGNHVNFFA
AGGTSLQAIQLSSRVNAVFGVALPIADFFATPTVAGLAGLVDAARSGQEARRDALAEALD
LVEGSSDEELAELAASLDGEGTR
selected fasta
>non-ribosomal peptide synthetase [Azi3]
ATGAGTCAACAGAGCGAGCTGCTTCGCCGACTGCGCCGGCTCCCGGGGGAGCGCCGGGAC
GCGCTGTTGGCTCAGCTGGAGGATGTGTCCGCCGGAGCCCGGTCCGCCCCCCTGTCCTAC
CGGCAGGAGCAGCTGTGGCTGTTCGACCGGTTCGCCCCCAGCAGCCTGGCCTACGGGCTC
GGCTTCGCCCTCCCGATCGAGGGCCGGCTCGACACGGAAGCGCTGGGCGCGGCGCTGGCC
GATCTGCTGCAGCGCCACACCGTGCTGCGCTCGACGTTCTCCGACGAGGACACCCGGGGC
ACCCAGCGGCTGAAGCCGGTGCCCCGCATCCGTCTGGAGACCGAGGACGTTCCCGCCGAG
CGGTTGCACCCGCGCGCCGACGAGGTGGTGCAGGGCGAGCTGCGCCGGGGATTCGACCTC
AACTCCGGCCCGATGGTCCGTTTCCGGCTGCTGCGGTCCGCCCCCGACCTGCACCTGCTG
GTGCTGACCAGCCACTCCATCGTCCTCGACCCGCGCTCCGCACGCGTGCTGCAGGGCGAT
CTGGCCCACGCCTACCGCGCCCGGGCGGCGGGCGCAGCGCCGGCCTGGGCGCAGGATCCG
GTGGAGTTCGGTACCTACGCGGCCTGGCAGCGGGCCTGGTGCGAGCAGGACGCCGCCCGC
GAGGCGACCACCTACTGGCGTGACGCCCTCGCGGGCTGGGAGAGCACCGAGCTGCCCACC
GACCTCCCCCGTCCGCGGCTGATCGACCTGAGCAGCGACGTCGTCCGGCGGCCGCTGCCG
CCCGGACTGGCGGCGGCCGCCGACGACTTGGCGACCGGGAGCGGCGCCGAACGGCGGGAC
GTCCTGCTGGCCGCTTTCCTGGTCGTGATCGCCCGCCACACCTCCCGGGGCGACCTCACC
ATCGGACTTCCCCAGGACGTCGCGGGCCCGTACGACGAGAGCCGACTCGTCGGTGACATC
GGAAACCTGCTGCCGCTGCGGGTCGAACTGGACGAGGCGGACAGCTTCGCCGCCGTGGTG
GCCCGGGTGCGCGACGCCCGGCGGGACGGCCTGCACCACGCGAGTCTGCCGTTCAAGCTG
ATCCTGGACGCACTCGGGGTGGAGCCCGACGCCGGCCGGCTGCCCCTGGTACAGATCGGC
TTCACGACCCCGGTCGCGGGCGGCGGACCGGCCGAGGCCGACGGGGTGCGCTTCGGCTGC
GAGCAGATCGACGCCGGGTCCGGCGCGTTCGAACTCGCGCTGGAGGTCACGGACGGTGAA
CAGCCCACCGTGGCCGTGCGGTTCGCCACCTCTCTCTACCGGCGGGCCACGGCCACCCGC
GTCCTGGACCGTTTCCTGCAGACCCTGGACGCGCTCTGCCGACAGCCGCGGCGTCCCCTG
GGAGACGCCCCGCTGGGCACGCCGCAGGAGCACGCCGACGTACGGGAGCGCTGGAACGAG
CCGATCGGCGCGCCTGCCGACACCCCGCTGCACGACCTGTTCGCCGAGGTCGTCGCGGCG
GCTCCGGACAACACCGCGCTGCTGTGGAAGGGCGGCTCCCTGTCCTACGGCGAACTGGAC
GCCTGGTCCTGGCGGATCGCGCGGCGGCTGACGGCCGCCGGGGTGGGGCTCGGCGACCTC
GTGCCGGTGGTGATGCAGCGGGGCCCGGCCCTGCTCGCCGCCGTCCTCGGTGTCCTGCGG
GCCGGGGGCGCCTACGTGCCGGTGGACGCCGGCCAGCCCGACGAGCGGCTGGCGATGATC
CTGGAGGACACCGGGGCCAGGACCGTGGTGGTCTCCCCCGGTACGGCGGACCGGGTCCCC
GGTCCGCTGGCGCTGGTCGTCGGTGCCGATCCGCTGGACGGACCGCAGGCGGAGGAGGAG
CCGCCTGCGGTCGTCGTGCCGGCCGCCTCCGCGGCGTACGTCATCTACACCTCGGGGTCC
ACCGGCCGTCCCAAGGGGGTGGTGGTCGAGCACCGCAACGCGGCCAACTTCACCCGCACC
GTCCGGGACATGTTCGACCTCACTCCCGAGGACCGGGTCCTGCATTTCGCCTCCCCCGGC
TTCGACGTCTCGGTGTTCGAGATCTTCGGAACCCTGCTCAGCGGCGCGGTCCTGTACGTA
CTGGACGAGGACCAGCGGCGCTCCGTGGACGCCCTCGACGCAGTCCTGCAGGAGCAGCGG
ATCACCGTCATCGACCTGCCCCCGGCGATCATGGATCTGCTCGCTCCCGAGAACTATCCC
GACCTGCGGGTGGCGTTCGTCGGGGGCGAGGCGTTCACCGGCGAGCTGACCACGCGGTGG
GCCCGTGGCCGGAGCTTCTGGAACGGGTACGGCCCGACGGAGACCACGGTGACCGTGGTG
GCCAAGCGATGCGAGGGCGAGTGGACGGCCTCGCCGCCGATCGGCCGGGCGATGGCCAAT
CACCGTGCCTACGTGGTGGACGGCGCCGGTGCGTCGTTGCTCCCGGCCGGCGCGGTCGGT
GAACTCGCCATCGCCGGGCTGGGCGTGGCGCGCGGCTACCTGGGCCAGCCCGCCCTCACC
GCCGACCGGTTCCGGCCGGACCCCGACGGGCCTCCCGGTTCCCGCCGCTACCTCACCGGT
GACCTGGCCGTCTGGGACGAGGCAGGGGACCTGTGCTTCGTCGGCAGGGCCGACCGGCAG
GTGAAGGTCCGCGGCGTGCGGATCGAGCTGGGCGAGGTGGAGGCCGCGCTGCAGGCGGTG
GACGGAGTCGCCCAGGCGGTGGCGGAGGTGGCGACCGATCCGCGCACCGGCGCGCTGCTG
GTGGCGTACGTGGTGCCGGAGCGTCCCGGCGAGCTGCAGCTGGACACGATCCGCTCCGCG
GTGGCGCGCCGGCTGCCGGCCGCGATGGTCCCCTCCGTCCTGGTGCCGCTCGACGCGGTC
CCGCTGACCCCGAGCGGCAAGATCGACCGACGGCGGCTGCCCGCCGTGGAGTTCGCCGTG
GTGGACGAACTCTCCGAGGCCGACGAGGAGAACAGCACGCCCACCGAGCGCACGATGCGC
GAGGAGGTGTTCACCCCGCTGCTGGGCGCCCGGATCGGCAATCACGTGAACTTCTTCGCG
GCGGGCGGCACCAGTCTTCAGGCGATCCAGCTGTCCTCCCGCGTGAACGCCGTGTTCGGG
GTGGCCCTGCCGATCGCCGACTTCTTCGCCACTCCCACCGTGGCCGGTCTGGCCGGGCTC
GTCGACGCGGCGCGTTCGGGCCAGGAAGCGCGGCGGGACGCGCTGGCGGAGGCCCTGGAC
CTGGTGGAGGGGAGCAGCGACGAGGAACTCGCCGAGCTGGCCGCCTCGCTGGACGGGGAG
GGGACGCGTTGA
[4] C35..331
[4] A515..908
[4] aziridino[1, 2a]pyrrolidinyl amino acid682..777
[4] PCP991..1062
[4] C103..993
[4] A1543..2724
[4] aziridino[1, 2a]pyrrolidinyl amino acid2044..2331
[4] PCP2971..3186

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[515-907] 4.2e-103 PF00501
PF00501   AMP-binding
IPR001242 Condensation domain (Domain)
[35-331] 4.9e-44 PF00668
PF00668   Condensation
IPR009081 Acyl carrier protein-like (Domain)
[988-1063] 1.49999977583352e-13 SSF47336
SSF47336   ACP_like
[1008-1061] 2.8e-10 PF00550
PF00550   PP-binding
[991-1062] PS50075
PS50075   ACP_DOMAIN
[986-1067] 1.2e-18 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
IPR010071 Amino acid adenylation domain (Domain)
[515-908] 7.90000000000016e-127 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020845 AMP-binding, conserved site (Conserved_site)
[634-645] PS00455
PS00455   AMP_BINDING
SignalP
[1-1] Signal-anchor
Eukaryota   
TMHMM No significant hit
Azino_00030 Azino_00030   Azino_00050 Azino_00050
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