Azino_00050 Azino_00050   Azino_00070 Azino_00070

Azino_00060 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction12,585 / 11,548 / - [in whole cluster]
12,585 / 11,548 / - [in contig]
Locationcomplement(11548..12585) [in whole cluster]
complement(11548..12585) [in contig]
TypeCDS
Length1,038 bp (345 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
Product3-hydroxy-5-methyl-naphthoic acid C-3 O-methyltransferase
Product (GenBank)Azi5
GeneaziB2
Gene (GenBank)azi5
EC number2.1.1.-
Keyword
  • 3-methoxy-5-methyl-naphthoic acid
Note
Note (GenBank)
  • O-methyltransferase
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
[20485749] Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into the antitumor antibiotic azinomycin B. (Mol Biosyst. , 2010)
comment
[PMID:18635006]
Azinomycin B生合成遺伝子クラスターの報告。

aziB2: O-methyltransferase

配列解析に基づき、azinomycin B骨格組み立ての最初のbuilding blockである3-methoxy-5-methyl-naphthoic acid(NPA)は、5-methyl-NPAがAziB1(putative P450 hydroxylase)によりC3でOH基を導入し、続いてAziB2(putative O-methyltransferase)によりメチル化されてできると考えられた。

S.albusでaziBのみを異種発現させると5-methyl-NPAが、aziB, B1, B2を発現させると3-methoxy-5-methyl-NPAが産生された。

この結果からaziB1, aziB2の機能が確認され、azinomycin B骨格を組み立てるNRPS AziA1の基質として3-methoxy-5-methyl-NPAが強く支持された。


[PMID:20485749]　abstract + Supplemental Data
O-methyltransferase AziB2のin vivo and/or in vitroでの特徴づけをしている。
AziB2がSAMを利用して3-hydroxyl-5-methyl-NPAから3-methoxy-5-methyl-NPAを形成することを複数の実験で確認している。

close this sectionSequence

selected fasta
>3-hydroxy-5-methyl-naphthoic acid C-3 O-methyltransferase [Azi5]
MAAGSGSGTPPGTPPPTLLTDLATGLWKTQTLTAAIETGLFEALAAGDADAPETAQRLGI
GKRPAEILLTACTALGLLEQRDGRYRNTAVAAHYLVPGLPDYFGGYVQMVARYTAPGWLR
ATEAVRTDAPTKPVPDPDRNMFEEGNRPESFWEGLFTFSTLTARQLAASVDLSGVRRIMD
VGGGAGATLIELCRQHPHLSGTVVDLPHVCALAGERIAAAGMTGRIDTAAADFFADPLPS
GHDAVLLSMILHDWDESQNRKILASCLDALPSGGTVLISELLVDDDKSGPVDAALMSMNM
LVGTWGRNYTGAEYTDWLRDAGCSEVRTVRFASPGANGVVAGVKA
selected fasta
>3-hydroxy-5-methyl-naphthoic acid C-3 O-methyltransferase [Azi5]
ATGGCCGCCGGTTCCGGCTCCGGGACGCCTCCCGGCACCCCGCCTCCGACCCTGCTGACC
GACCTGGCGACGGGGCTGTGGAAGACCCAGACCCTGACCGCGGCGATCGAGACAGGCCTC
TTCGAAGCCCTGGCCGCCGGCGACGCCGACGCTCCCGAGACGGCGCAACGGCTCGGCATC
GGCAAGCGGCCGGCCGAGATCCTGCTGACCGCGTGCACCGCGCTCGGGCTGCTGGAGCAG
CGGGACGGGCGCTATCGGAACACCGCCGTCGCGGCGCACTACCTGGTGCCCGGCCTGCCC
GACTACTTCGGCGGTTACGTGCAGATGGTGGCCCGGTACACCGCGCCGGGGTGGCTTCGG
GCGACCGAGGCGGTACGCACCGACGCCCCCACCAAGCCGGTGCCCGACCCGGACCGGAAC
ATGTTCGAGGAGGGCAACCGGCCGGAGTCCTTCTGGGAGGGGCTCTTCACCTTCTCCACC
CTGACCGCCCGCCAGCTGGCGGCGTCGGTGGACCTCTCCGGTGTCCGCCGGATCATGGAC
GTCGGCGGCGGCGCGGGCGCCACGCTGATCGAACTGTGCCGGCAGCACCCGCACCTGAGC
GGCACCGTCGTCGACCTGCCGCACGTGTGCGCACTCGCCGGCGAACGCATCGCCGCGGCC
GGTATGACCGGACGCATCGACACGGCCGCGGCCGACTTCTTCGCGGATCCGCTGCCCTCC
GGGCACGACGCCGTCCTGCTGTCGATGATCCTGCACGACTGGGACGAGAGCCAGAACCGG
AAGATCCTCGCCTCCTGCCTGGACGCGCTGCCCTCCGGCGGCACCGTCCTGATCAGTGAG
CTGCTGGTGGACGACGACAAATCCGGCCCGGTGGACGCGGCGCTGATGAGCATGAACATG
CTGGTGGGCACCTGGGGACGGAACTACACCGGCGCCGAGTACACCGACTGGCTGCGCGAC
GCGGGCTGCTCCGAGGTGCGCACGGTCCGGTTCGCCTCACCGGGGGCGAACGGTGTGGTG
GCGGGGGTGAAGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001077 O-methyltransferase, family 2 (Domain)
[84-318] 6.80000000000006e-44 PF00891
PF00891   Methyltransf_2
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[14-92] 1.1e-22 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016461 O-methyltransferase, caffeic acid-type (Family)
[5-344] 3.39999972437719e-08 PIRSF005739
PIRSF005739   O-mtase
SignalP No significant hit
TMHMM No significant hit
Azino_00050 Azino_00050   Azino_00070 Azino_00070
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