Azino_00070 : CDS information

Location

Organism	Streptomyces sahachiroi
Strain	NRRL 2485 (=NBRC 13928)
Entry name	Azinomycin B
Contig	EU240558
Start / Stop / Direction	13,792 / 12,587 / - [in whole cluster] 13,792 / 12,587 / - [in contig]
Location	complement(12587..13792) [in whole cluster] complement(12587..13792) [in contig]
Type	CDS
Length	1,206 bp (401 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450 5-methyl-naphthoic acid C-3 hydroxylase
Product (GenBank)	Azi6
Gene	aziB1
Gene (GenBank)	azi6
EC number
Keyword	3-methoxy-5-methyl-naphthoic acid hydroxylate
Note
Note (GenBank)	cytochrome P450
Reference	ACC B4XY99 PmId [18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008) [20485749] Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into the antitumor antibiotic azinomycin B. (Mol Biosyst. , 2010) comment [PMID:18635006] Azinomycin B生合成遺伝子クラスターの報告。 aziB1: Cytochrome P450 hydroxylase 配列解析に基づき、azinomycin B骨格組み立ての最初のbuilding blockである3-methoxy-5-methyl-naphthoic acid(NPA)は、5-methyl-NPAがAziB1(putative P450 hydroxylase)によりC3でOH基を導入し、続いてAziB2(putative O-methyltransferase)によりメチル化されてできると考えられた。 S.albusでaziBのみを異種発現させると5-methyl-NPAが、aziB, B1, B2を発現させると3-methoxy-5-methyl-NPAが産生された。この結果からaziB1, aziB2の機能が確認され、azinomycin B骨格を組み立てるNRPS AziA1の基質として3-methoxy-5-methyl-NPAが強く支持された。 --- [PMID:20485749]　abstract + Supplemental Data P450 hydroxylase AziB1のin vivo and/or in vitroでの特徴づけをしている。 AziB1がP450 enzymeであること、5-methyl-NPAを基質として3-hydroxyl-5-methyl-NPAを形成することを実験的に確認している。

Sequence

>cytochrome P450 [Azi6]
MTTEAADATDRLVTAFDHHDPGYTPRTAERINTEIRERGVTWSPAYGGIWILSRYADVRA
ALTDWRTYSSARGVHFPRAEGMPMFSPIDYDPPAQRGIRERMAAPMTGDAVSAMVPELRR
MVARLLAPLAGRGHGDLMAEFAEPFAIEVLGVAFGLSESCRARIREATRTMWTYISADRD
ASKFWPAFHALLAEEVERVRDEPDGSYLARLAAMRRDGSPLPDEELYSIIVSFCVAGHDN
TMNSITRLVHTLAQDPALQLRLRREPELRPAVAEEALRRWCPTDRFTRVTTREVTVAGTV
IPAGARVVLLFDAANRDPEKFPDPDTFDPDRGNSHQHLSFGHGIHHCMGVHLARAEFAAV
LDELSRLPLFDLEQPSDLHFENGRHIMFDRVSVRFRTGEEH

>cytochrome P450 [Azi6]
ATGACCACCGAAGCCGCGGACGCCACCGACCGGCTGGTGACCGCTTTCGACCACCACGAC
CCCGGATACACCCCGCGGACCGCGGAGCGGATCAACACGGAGATCCGTGAGCGGGGGGTG
ACGTGGTCACCGGCGTACGGCGGCATCTGGATACTGTCCCGGTACGCGGACGTGCGCGCC
GCCCTGACGGACTGGCGTACCTACAGCTCGGCCCGCGGCGTGCACTTCCCGCGCGCCGAG
GGGATGCCGATGTTCTCACCCATCGACTACGACCCCCCGGCGCAGCGCGGGATACGTGAG
CGGATGGCCGCTCCGATGACGGGCGACGCCGTCTCCGCGATGGTGCCTGAGCTGCGCCGC
ATGGTCGCGCGGCTGCTCGCGCCGCTGGCCGGGCGCGGCCACGGGGACCTGATGGCCGAG
TTCGCGGAGCCGTTCGCCATCGAGGTGCTCGGAGTGGCCTTCGGGCTTTCCGAGAGCTGC
CGCGCCCGGATCCGCGAGGCGACGCGGACGATGTGGACCTACATCTCGGCCGACCGGGAC
GCGAGCAAGTTCTGGCCCGCCTTCCACGCCCTGCTGGCCGAGGAGGTCGAGCGGGTGCGC
GACGAGCCGGACGGCAGCTATCTCGCCCGGCTCGCGGCCATGCGGCGGGACGGTTCGCCG
TTGCCGGACGAGGAGCTGTACTCGATCATCGTCAGCTTCTGCGTCGCCGGGCACGACAAC
ACGATGAACTCGATCACCCGCCTCGTGCACACGCTGGCCCAGGATCCCGCACTCCAACTG
AGGCTGCGCCGCGAGCCGGAGCTGCGCCCGGCCGTCGCGGAGGAAGCCCTGCGCCGCTGG
TGTCCCACCGACCGGTTCACACGGGTCACCACCCGTGAGGTCACCGTCGCGGGCACCGTC
ATTCCGGCGGGTGCCCGGGTGGTGCTCCTGTTCGACGCTGCCAACCGGGACCCGGAGAAG
TTCCCGGATCCCGACACCTTCGATCCCGACCGCGGCAACTCCCATCAGCACCTCAGCTTC
GGGCACGGCATCCATCACTGCATGGGGGTGCACCTGGCCCGCGCCGAGTTCGCCGCCGTG
CTCGACGAACTGTCCCGGCTGCCGCTGTTCGACCTGGAGCAGCCGAGCGACCTGCATTTC
GAGAACGGTCGGCACATCATGTTCGACCGGGTGTCGGTGCGGTTCCGCACCGGAGAGGAG
CACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Azino_00060 Azino_00080