Azino_00070 Azino_00070   Azino_00090 Azino_00090

Azino_00080 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction15,756 / 13,873 / - [in whole cluster]
15,756 / 13,873 / - [in contig]
Locationcomplement(13873..15756) [in whole cluster]
complement(13873..15756) [in contig]
TypeCDS
Length1,884 bp (627 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)Azi7
GeneaziA1
Gene (GenBank)azi7
EC number
Keyword
Note
Note (GenBank)
  • NRPS(A-PCP)
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
[20485749] Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into the antitumor antibiotic azinomycin B. (Mol Biosyst. , 2010)
comment
[PMID:18635006]
Azinomycin B生合成遺伝子クラスターの報告。
aziA1-A5はmodular NRPS genesで、推定産物は非定型的なNRPS systemを構成する。

aziA1: NRPS (AL-PCP)

AL=acyl-CoA ligase
AziA1-ALの基質特異性を与えるコードはGIHTTGSQIK(unusual substrate)と同定。

AziA1は、acyl-CoA ligase(AL)とPCPに関与する2つの触媒活性を持つbifunctional proteinであり、
おそらく 3-methoxy-5-methyl-naphthoic acid(NPA)の活性化を担う。

---
[PMID:20485749]　abstract only
NRPS AziA1の基質特異性について報告あり。

di-domain NRPS AziA1 は、普通でないA domainを介して3-methoxy-5-methyl-NPAを特異的に取り込み、azinomycin Bの骨格形成を開始する。

AziA1 は自然なstarter unitの類似体も活性化したことから、NPA部分で異なる新規azinomycin類似体産生の可能性が示唆されている。

close this sectionPKS/NRPS Module

B1 3-methoxy-5-methyl-naphthoic acid
A44..457
PCP540..607

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [Azi7]
MIRQQDAPAVPGAGPATTVVSRIELFARDTPDAPAVRHGERELTYRDLDRRAGQLASELV
ESGVRHGDMVAMYLGRSIEWVVGMLGCLKTGGVCMPLDPAVPAERFDRAVAAARPAAVVT
AVPGRGTGNPLPALPAGLPVVTLDASAPLPAETPGGGLAPRAQPRCEDLAYAMFTSGSSG
EAKIVLAQHSWLALSAARSAAVNATTAADRGSWLGAAGAGIALHEVGGLLWQGAQLVIGD
HEVIASPPALRDWLLAERITQSFVITPVGEVLQNLPWPRECALRLLTLGGDRLNRWGPAD
LPFEVAVSYGSLEAFQIANSCHPWSQRCTPATATDADRASAPPVGRPIAGVTVHLLEDDG
LTPVVDGIGEVWIDSDCLSLGYLGDPAQTADRFRPNPFGAAGSRIYRSGDAGRFRPDGIL
EHHGRIDNIVKIRGHRVELGDVEWALGQHPDVDQVAVVATWDGDQRRLVACFVAAREIAP
LELRDHAVERLPDWMVPVAYVQLDEFPLNTSRKIDRRRLPPADWSRGRPSRPWRAPAPGA
QTELARLFADLLQVDRVGADDHFVELGGDSLLLARLQSRIEQRFGVRIELPDLMAGPTVV
ELAGRIPGATAGRTGAAALPPIVPRRR
selected fasta
>non-ribosomal peptide synthetase [Azi7]
ATGATCCGCCAGCAGGACGCTCCGGCCGTACCCGGGGCCGGGCCGGCCACCACGGTGGTC
TCCCGTATCGAGCTGTTCGCCCGCGACACCCCCGATGCCCCCGCGGTCCGGCACGGCGAA
CGGGAGCTGACCTACCGGGACCTGGACCGGCGTGCCGGGCAACTCGCCTCGGAGCTGGTC
GAGTCGGGAGTACGACATGGCGACATGGTCGCCATGTACCTGGGCCGCTCCATCGAGTGG
GTGGTCGGCATGCTCGGCTGCCTGAAGACGGGCGGTGTGTGCATGCCGCTGGACCCGGCC
GTACCCGCGGAACGGTTCGACCGGGCGGTCGCCGCGGCGCGCCCCGCGGCCGTCGTGACC
GCGGTTCCCGGCCGTGGTACGGGCAACCCCCTGCCCGCACTCCCCGCCGGGCTGCCCGTG
GTCACCCTCGACGCGAGCGCTCCGCTCCCCGCAGAGACACCCGGCGGCGGACTCGCCCCG
CGTGCGCAACCACGGTGCGAGGACCTCGCGTACGCGATGTTCACCTCCGGTTCCTCCGGT
GAGGCCAAGATCGTGCTGGCCCAGCACTCCTGGCTGGCGCTCAGCGCCGCCCGCAGCGCC
GCGGTGAACGCCACCACCGCCGCCGACCGCGGTTCCTGGCTCGGCGCGGCCGGCGCCGGG
ATCGCGCTGCACGAGGTCGGCGGTCTGCTCTGGCAGGGGGCCCAGCTCGTGATCGGCGAC
CACGAGGTGATCGCGTCACCGCCCGCCCTGCGGGACTGGCTGCTCGCCGAGCGGATCACC
CAGAGTTTCGTGATCACCCCCGTCGGCGAGGTCCTGCAGAATCTGCCCTGGCCGCGCGAG
TGCGCGCTGCGGCTCCTCACCCTGGGCGGTGACCGGCTGAACCGGTGGGGGCCCGCGGAC
CTGCCGTTCGAGGTCGCCGTCTCCTACGGCAGCCTGGAAGCCTTCCAGATAGCCAACTCG
TGCCATCCCTGGTCCCAGAGGTGCACTCCCGCCACGGCCACCGACGCCGACCGCGCCAGC
GCCCCGCCTGTCGGCCGGCCGATCGCCGGGGTCACCGTCCACCTGCTGGAGGACGACGGC
CTGACACCGGTCGTCGACGGGATCGGCGAGGTGTGGATCGACTCGGACTGCCTCAGTCTC
GGGTATCTGGGCGATCCGGCGCAGACCGCGGACCGGTTCCGGCCCAACCCGTTCGGCGCC
GCGGGCAGCCGCATCTACCGCTCGGGCGACGCGGGCAGGTTCCGTCCCGACGGGATCCTG
GAGCACCACGGCCGGATCGACAACATCGTGAAGATACGCGGCCACCGGGTGGAGCTCGGG
GATGTCGAGTGGGCGCTCGGGCAGCACCCGGACGTCGACCAGGTCGCCGTGGTGGCGACC
TGGGACGGCGACCAGCGCCGCCTCGTCGCCTGCTTCGTCGCGGCACGCGAGATCGCGCCG
CTGGAGCTGCGCGACCACGCGGTGGAACGGCTGCCGGACTGGATGGTCCCGGTCGCGTAC
GTGCAACTGGACGAGTTCCCGCTGAACACGTCCCGCAAGATCGACCGCCGCAGGCTGCCC
CCGGCCGACTGGTCCCGGGGACGTCCGTCGCGCCCCTGGCGGGCACCCGCACCCGGAGCG
CAGACCGAGCTGGCCCGCCTGTTCGCGGATCTGCTGCAGGTGGACCGGGTCGGAGCCGAC
GACCACTTCGTGGAACTCGGCGGCGACTCCCTGCTGCTGGCCCGGTTGCAGAGCAGGATC
GAGCAGCGCTTCGGCGTCCGGATCGAACTGCCGGACCTGATGGCCGGCCCGACCGTCGTC
GAACTGGCCGGACGCATCCCGGGGGCCACCGCCGGGCGCACCGGCGCGGCCGCGCTGCCG
CCCATCGTGCCCCGGCGCCGCTGA
[1] A44..457
[1] 3-methoxy-5-methyl-naphthoic acid220..317
[1] PCP540..607
[1] A130..1371
[1] 3-methoxy-5-methyl-naphthoic acid658..951
[1] PCP1618..1821

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[44-457] 3.49999999999995e-75 PF00501
PF00501   AMP-binding
IPR009081 Acyl carrier protein-like (Domain)
[532-624] 5.79999653854781e-17 SSF47336
SSF47336   ACP_like
[540-607] PS50075
PS50075   ACP_DOMAIN
[543-606] 1.9e-15 PF00550
PF00550   PP-binding
[531-608] 2.4e-20 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
SignalP No significant hit
TMHMM No significant hit
Azino_00070 Azino_00070   Azino_00090 Azino_00090
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