Azino_00210 : CDS information

Location

Organism	Streptomyces sahachiroi
Strain	NRRL 2485 (=NBRC 13928)
Entry name	Azinomycin B
Contig	EU240558
Start / Stop / Direction	29,678 / 30,658 / + [in whole cluster] 29,678 / 30,658 / + [in contig]
Location	29678..30658 [in whole cluster] 29678..30658 [in contig]
Type	CDS
Length	981 bp (326 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative LysW-glutamyl-phosphate/LysW-aminoadipyl-phosphate reductase
Product (GenBank)	Azi21
Gene	aziC4
Gene (GenBank)	azi21
EC number
Keyword	aziridino[1,2a]pyrrolidinyl amino acid
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)	N-acetyl-gamma-glutamyl-phosphate reductase
Reference	ACC B4XYB3 PmId [18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008) comment Azinomycin B生合成遺伝子クラスターの報告。 aziC4: N-acetyl-gamma-glutamyl-phosphate reductase AziC2-C11は、3つめのbuilding blockであるaziridino[1,2a]pyrrolidinyl amino acidの生合成に関与すると想定される。 AziC3, AziC4はオルニチン生合成経路の酵素と相同性を示す。AziC4はN-acetylglutamyl-gamma-semialdehydeを形成するための還元に関与する可能性が示唆された。
Related Reference	ACC O50146 PmId [19620981] ( , ) comment Blast id44% Thermus thermophilus HB27_argC2(argC, lysY) N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (EC 1.2.1.-/1.2.1.38) LysW, LysXを用いるlysine生合成経路の報告。異種性発現、精製して得たLysW, LysX, LysY, LysZ, LysJ and LysKをputative cofactorsと共に反応させると、相当量のlysineが検出された。どれかひとつでも酵素を省略すると、lysine産生は観察されない。 LysZによってリン酸化されたalpha-aminoadipate(AAA)部分を含む中間体、その後LysYによって形成されるAAA semialdehyde部分を含む中間体は、実験はしてみているがそれらがおそらく不安定であったため検出できていない。
Family/Domain	FD IPR000706 comment [IPR000706] N-acetyl-gamma-glutamyl-phosphate reductase, type 1 (Family) HAMAP MF_00150 N-acetyl-gamma-glutamyl-phosphate reductase(EC 1.2.1.38)_argC

Sequence

>putative LysW-glutamyl-phosphate/LysW-aminoadipyl-phosphate reductase [Azi21]
MKVSVVGAAGYTGGELLRLVHAHPKLELAQALSRRHAGRPVAEVHPNLHHLALAFRSPEE
LEPVDAAFLALPSGASAEVETAVTGRATTIVDLSPDFRGGRPSADGTAYRTGLPELFAGE
LVGATRISVPGCMATAAVLALAPVQRSGLVAGDVVVDARAGSSGSGAEPSPASHHAERGN
AFRVYKPAGHRHEKEIAALAGVRARMTVTAVPTVRGAQVLCHVTPPRPVTRREVLHVLQR
AYRDHPFVRIVSRGRGVHRMPDPQFLGGTNFVDIGCAVDEDGGRIVLVAALDNLVKGAAG
GGVQSLNVAAGMPQDAGLEFTGLHPA

>putative LysW-glutamyl-phosphate/LysW-aminoadipyl-phosphate reductase [Azi21]
GTGAAGGTGAGCGTCGTCGGCGCGGCCGGATACACCGGAGGTGAACTGCTGCGGCTGGTG
CATGCCCACCCCAAGCTGGAACTGGCACAGGCGCTTTCGCGGCGGCATGCGGGCCGGCCG
GTGGCAGAGGTGCACCCCAACCTGCATCATCTCGCCCTGGCCTTCCGGTCCCCCGAGGAG
CTGGAGCCGGTGGACGCGGCGTTCCTGGCCCTGCCCTCCGGCGCCTCCGCCGAGGTGGAG
ACAGCGGTGACGGGCCGGGCCACGACCATCGTGGACCTGAGCCCCGATTTCCGGGGCGGC
CGGCCCTCGGCGGACGGCACCGCCTACCGGACCGGCCTGCCCGAGCTGTTCGCCGGCGAA
CTCGTCGGCGCCACCCGGATCAGCGTGCCCGGATGCATGGCCACCGCGGCCGTCCTGGCC
CTGGCTCCGGTCCAGCGCAGTGGTCTGGTGGCCGGCGACGTGGTGGTGGACGCCCGGGCC
GGGTCGTCGGGCTCCGGAGCGGAGCCGAGCCCTGCCTCGCACCACGCGGAGCGCGGCAAC
GCCTTCCGGGTCTACAAGCCCGCGGGACACCGGCACGAGAAGGAGATCGCCGCACTGGCC
GGGGTGCGTGCCCGGATGACGGTGACCGCCGTCCCCACGGTGCGCGGCGCGCAGGTGCTC
TGCCATGTCACACCCCCGCGGCCGGTCACCCGCCGCGAGGTGCTGCACGTGCTGCAGCGC
GCCTACCGAGACCACCCGTTCGTCCGGATCGTGTCCCGCGGGCGCGGGGTGCACCGGATG
CCCGATCCGCAGTTCCTCGGCGGCACCAATTTCGTCGACATCGGTTGCGCCGTGGACGAG
GACGGCGGCCGCATCGTCCTGGTCGCCGCGCTGGACAACCTCGTGAAGGGGGCCGCGGGG
GGCGGGGTGCAGAGCCTGAACGTGGCGGCCGGCATGCCACAGGACGCCGGACTGGAATTC
ACCGGACTGCACCCCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000534 Semialdehyde dehydrogenase, NAD-binding (Domain) SM00859 Semialdehyde dehydrogenase, NAD bindin PF01118 Semialdhyde_dh IPR000706 N-acetyl-gamma-glutamyl-phosphate reductase, type 1 (Family) TIGR01850 argC: N-acetyl-gamma-glutamyl-phosphate reductase MF_00150 ArgC_type1 IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	No significant hit
TMHMM	No significant hit

Azino_00205 Azino_00220