Azino_00230 Azino_00230   Azino_00250 Azino_00250

Azino_00240 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction32,741 / 33,559 / + [in whole cluster]
32,741 / 33,559 / + [in contig]
Location32741..33559 [in whole cluster]
32741..33559 [in contig]
TypeCDS
Length819 bp (272 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)Azi24
GeneaziC8
Gene (GenBank)azi24
EC number
Keyword
  • aziridino[1,2a]pyrrolidinyl amino acid
Note
Note (GenBank)
  • acyl-CoA dehydrogenase/reductase 33636
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
comment
[PMID:18635006(2008)]
Azinomycin B生合成遺伝子クラスターの報告。

aziC8: Acyl-CoA dehydrogenase/reductase

AziC2-C11は、3つめのbuilding blockであるaziridino[1,2a]pyrrolidinyl amino acidの生合成に関与すると想定される。

AziC8は、short chain acyl-CoA dehydrogenase/reductasesのfamilyと相同性を示す。alpha-beta dehydrogenationは、1つめの窒素を含んだ5員環の閉環を開始するために要求されると示唆される。

azi gene cluster内に明白な候補遺伝子はないが、2つめのaziridine環の閉環のため、末端のhydroxylは優れた脱離基を形成することで活性化されうると考察している。
Related Reference
ACC
Q9RA05
PmId
comment
Blast id36%
Rhodococcus erythropolis_limC
(-)-trans-carveol dehydrogenase

R. erythropolis DCL14のcarveol → carvone (dichlorophenolindophenol-dependent oxidation)を触媒するnicotinoprotein= carveol dehydrogenase (CDH)を精製、酵素学的性質を解析している。

LimCはlimonene分解に関与する酵素をコードすると同定された。
ACC
Q27YP6
PmId
comment
Blast id36%
Streptomyces hygroscopicus
Putative short chain dehydrogenase

HPLCを用いてdehydrogenase活性、逆反応(reductase活性)、酵素学的解析を行い、Hyg26は広い基質特異性を持つ NAD+/NADH-dependent oxidoreductaseであることが示され、5''-dihydrohygromycin Aとhygromycin Aの可逆性の相互変換を触媒する事を示した。

close this sectionSequence

selected fasta
>putative oxidoreductase [Azi24]
MLDGDVALITGAARALGRSHALAFAREGADLILLDRCAPDDGPYPMAGRRDLEETAEACR
RLGSRVLTGVADVRHQADVDKVVDAGLDAFGRIDVLLNNAGVLGPGGVRVHELSEEQWQL
ALDVNLSGTWRTCRAVLPHMVERGGGRIVNTASTGGRVAFERYANYVASKHGVIGLTKSI
ALEYGRHGIRANAVCPTTVADDAALGTRSTRAVAASMGAELPDYERTSAALHPIGRLVTA
AEVSAACLWLAGDRSGGVTGTELLVDGGFTAH
selected fasta
>putative oxidoreductase [Azi24]
ATGCTCGACGGCGACGTCGCCCTGATCACCGGTGCTGCTCGCGCGCTGGGCCGGTCGCAC
GCGCTGGCCTTCGCCCGCGAGGGCGCGGACCTGATCCTCCTCGACCGCTGCGCCCCCGAC
GACGGCCCCTACCCGATGGCCGGCCGGCGTGACCTGGAGGAGACCGCGGAAGCCTGCCGA
AGGCTCGGCAGCCGCGTCCTGACCGGAGTCGCCGACGTACGCCACCAGGCGGACGTGGAC
AAGGTGGTGGACGCCGGGCTCGACGCGTTCGGCCGGATCGACGTCCTGCTCAACAACGCG
GGCGTCCTCGGTCCCGGCGGCGTCCGCGTACACGAACTCAGCGAGGAACAGTGGCAGTTG
GCGCTGGACGTCAACCTGAGCGGCACTTGGCGCACCTGCCGCGCAGTGCTGCCGCACATG
GTCGAGCGGGGCGGCGGACGGATCGTCAACACCGCCTCCACCGGCGGACGCGTCGCCTTC
GAGCGGTACGCCAACTACGTCGCCTCCAAGCACGGCGTGATCGGACTGACCAAGTCCATC
GCGCTGGAGTACGGACGCCACGGCATACGTGCCAACGCGGTCTGTCCCACCACCGTGGCC
GACGACGCCGCACTGGGCACCCGCAGCACCCGCGCCGTGGCCGCGTCGATGGGCGCCGAA
CTGCCCGACTACGAGCGCACGTCGGCGGCGCTGCATCCGATCGGCCGACTGGTCACCGCC
GCGGAGGTGTCGGCCGCCTGCCTCTGGCTGGCCGGTGACCGGTCCGGCGGTGTCACCGGC
ACCGAACTCCTGGTGGACGGCGGCTTCACCGCCCACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[91-102] 4.89999951782359e-13 PR00080 [146-154] 4.89999951782359e-13 PR00080 [166-185] 4.89999951782359e-13 PR00080
PR00080   SDRFAMILY
[6-184] 1.3e-30 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[6-23] 8.20001307135067e-38 PR00081 [91-102] 8.20001307135067e-38 PR00081 [140-156] 8.20001307135067e-38 PR00081 [166-185] 8.20001307135067e-38 PR00081 [187-204] 8.20001307135067e-38 PR00081 [233-253] 8.20001307135067e-38 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[2-271] 5.00000000000001e-76 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[153-181] PS00061
PS00061   ADH_SHORT
IPR023985 Carveol dehydrogenase (Family)
[2-269] 6.60000000000002e-84 TIGR03971
TIGR03971   SDR_subfam_1
SignalP
[1-25] 0.215 Signal
Bacteria, Gram-positive   
[1-25] 0.218 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Azino_00230 Azino_00230   Azino_00250 Azino_00250
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