Azino_00320 : CDS information

Location

Organism	Streptomyces sahachiroi
Strain	NRRL 2485 (=NBRC 13928)
Entry name	Azinomycin B
Contig	EU240558
Start / Stop / Direction	48,254 / 49,513 / + [in whole cluster] 48,254 / 49,513 / + [in contig]
Location	48254..49513 [in whole cluster] 48254..49513 [in contig]
Type	CDS
Length	1,260 bp (419 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative cytochrome P450
Product (GenBank)	Azi32
Gene	aziC9
Gene (GenBank)	azi32
EC number
Keyword	aziridino[1,2a]pyrrolidinyl amino acid
Note
Note (GenBank)	cytochrome P450
Reference	ACC B4XYC4 PmId [18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008) comment Azinomycin B生合成遺伝子クラスターの報告。 aziC9: Cytochrome P450 hydroxylase AziC2-C11は、3つめのbuilding blockであるaziridino[1,2a]pyrrolidinyl amino acidの生合成に関与すると想定される。 AziC9はP450 monooxygenase familyに似ているので、C4位でのhydroxyl基導入のために働くことが支持される。配列解析のみ。
Related Reference	ACC Q9L9F9 PmId [11325587] Coumarin formation in novobiocin biosynthesis: beta-hydroxylation of the aminoacyl enzyme tyrosyl-S-NovH by a cytochrome P450 NovI. (Chem Biol. , 2001) comment Blast id30% Streptomyces sphaeroides_novI novobiocin生合成遺伝子 NovH, NovI の機能同定をしている。NovH, NovI をE.coli にて発現・精製し、coumarin形成の初段階であるtyrosineの活性化とoxygenationについて見ている。 NovI は、tyrosyl-S-NovH acyl enzymeに特異性をもつcytochrome P450-type monooxygenaseとして機能するhemeタンパク。 NovHとNovI は、beta-OH-Tyr中間体を形成するためにtyrosineの活性化とbeta-hydroxylationを担うとしている。

Sequence

>putative cytochrome P450 [Azi32]
MIISKPPFCPVQFQDSDLLNSGFHAATDMHALWADLRENQPLYRADPGGGREPFWVVTRH
EDVSRVLRSHGEFSSRRGTILCVLDLNTADIASDNMMADTDPPRHREFREPLNKAFAPSV
VATQERLLRELSQDLIQSVLEAGVYDIAHKTMMFPMAVTGTLMGLPPESWERLAELVMMT
IAYDDPDYSAGSAQATVRQARHELFEYFQKEYAQRSRDDVDPDVIGAMVGMDLSEGPMTQ
EQVLLNAFVLLIGANVTTPHALCTLMSVMAEHPEQYRAVQENPELRASCLQELLRWSSPV
TALMRYAVKDVEMHGRTIRAGEPVTAWIGAANRDERVFPDPYTFDVARRPNKHLAFGLGP
HYCIGANLAKVGLDIFLDELLERVESIEIAGEVKHVASHFVPGYKSMPVRFTPREAHVR

>putative cytochrome P450 [Azi32]
ATGATTATTTCCAAGCCCCCGTTCTGCCCGGTGCAGTTCCAGGACTCCGACCTGCTCAAC
TCCGGATTCCACGCCGCCACGGACATGCACGCGCTCTGGGCCGACCTGCGTGAGAACCAG
CCGCTCTACCGGGCCGACCCCGGCGGTGGCCGGGAGCCGTTCTGGGTCGTGACCCGCCAC
GAGGACGTCAGCCGGGTGCTGCGCAGCCACGGGGAGTTCTCCTCCCGGCGGGGCACGATC
CTGTGCGTGCTCGACCTCAACACCGCGGACATCGCGTCCGACAACATGATGGCCGACACC
GATCCGCCCCGGCACCGGGAGTTCAGGGAGCCGCTCAACAAGGCGTTCGCCCCCTCCGTG
GTCGCCACTCAGGAGCGGCTGCTCAGAGAACTGTCGCAGGACCTGATCCAGTCGGTCCTG
GAAGCCGGCGTGTACGACATCGCCCACAAGACGATGATGTTCCCGATGGCCGTCACGGGC
ACGCTCATGGGCCTGCCCCCCGAGTCCTGGGAAAGGCTCGCGGAGCTGGTCATGATGACG
ATCGCCTACGACGACCCCGACTACTCCGCCGGCAGTGCGCAGGCCACCGTGCGCCAGGCC
CGGCACGAGCTGTTCGAGTACTTCCAGAAGGAGTACGCGCAGCGGAGCCGGGACGACGTC
GACCCCGACGTCATCGGCGCGATGGTCGGCATGGATCTCTCGGAAGGACCGATGACGCAG
GAGCAGGTCCTCCTCAACGCCTTCGTGCTGCTCATCGGCGCGAACGTCACCACGCCGCAC
GCCCTGTGCACCCTGATGTCCGTGATGGCCGAACACCCGGAGCAGTACCGGGCCGTACAG
GAGAACCCCGAGCTGCGGGCGTCCTGCCTCCAGGAGCTCCTGCGCTGGTCCTCCCCGGTC
ACCGCGCTGATGCGGTACGCCGTCAAGGACGTGGAGATGCACGGCCGAACCATCCGGGCG
GGCGAGCCGGTCACCGCGTGGATCGGCGCGGCCAACCGGGACGAGAGGGTCTTCCCCGAC
CCCTACACCTTCGATGTCGCACGACGGCCGAACAAGCACCTCGCCTTCGGCCTGGGACCG
CACTACTGCATCGGCGCCAACCTGGCCAAGGTCGGCCTCGACATCTTCCTCGACGAACTC
CTGGAGCGGGTGGAGAGCATCGAGATCGCCGGCGAGGTGAAGCACGTCGCCTCCCACTTC
GTGCCCGGCTACAAGAGCATGCCGGTGCGGTTCACTCCTCGTGAGGCACACGTACGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Azino_00310 Azino_00330