Rever_00050 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	4,375 / 5,676 / + [in whole cluster] 4,375 / 5,676 / + [in contig]
Location	4375..5676 [in whole cluster] 4375..5676 [in contig]
Type	CDS
Length	1,302 bp (433 aa)

Annotation

Category	5.1 general function
Product	putative C4-dicarboxylate transport protein
Product (GenBank)	putative transporter
Gene	revO dctA
Gene (GenBank)	revO
EC number
Keyword
Note
Note (GenBank)
Reference	ACC G1UDT5 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment 配列解析によりrevOのfunctionをTransporterとしている。また、revO、revP、revQでRegulator and transporterとしている。実験情報は無し。
Related Reference	ACC P0A830 PmId [10482502] Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli. (J Bacteriol. , 1999) comment Blast 88th, id46%, 1e-102 Escherichia coli_dctA Aerobic C4-dicarboxylate transport protein aerobic C(4)-dicarboxylate transporter (DctA)の遺伝子(dctA)を不活性化したところ、fumarate や malateを炭素源としては増殖できなくなり、succinateでは増殖が低下した。Fumarateの取り込みが壊され、succinateの輸送が低下していることも見た。遺伝子制御についても調べている。
Related Reference	ACC P20672 PmId [2793824] ( , ) [2551890] ( , ) [2134335] ( , ) [8232193] ( , ) comment Blast 162nd, id47%, 3e-97 Rhizobium meliloti(Sinorhizobium meliloti)_dctA C4-dicarboxylate transport protein dctAの制御機構（PMID: 2793824）、dctAへのTn5挿入変異株ではC4-dicarboxylate transportが欠損し、dctAを相補することによって回復すること(PMID: 2551890)、 DctAのアミノ酸配列から推測される特徴(PMID: 2134335)、membrane topology (PMID: 8232193)が調べられている。

Sequence

>putative C4-dicarboxylate transport protein [putative transporter]
MSETVTIDAPPAAPKPPTPFYRDLAVQVLAGVALGVLVGNYAPSVGRHTQFLGDIFIHLI
QMVVGLIIFCTVTHGIASVRDLGKVGRIAVRALVYFEVITTAALIIGLVVVNVLHPGSGV
HIDLSHNGGAGTAPAKSEGFDDFLVGLVPTSAVQAFAQGDILQVLVFSVLFACGLASLGE
RSAPVLELVGTVQKTLFWIIGKVMRIAPLAAFGAIGYTVSKYGLGTLTSLGKLIGVFYLI
VLLFFLLVLWPVSRYAGVSLLKVLRYFRAELLLVLGTSSSESVFPQLTSKLERLGVEQSV
VGLVLPTAYSFNHDGTCLYFASVSVFLAQATGTDLGWQAQIGLLLILLLTSKGGAGVSGS
AIAVLALTLSSTHKIPVDSIALVLGIHSILSAAFVFTNIAGNCVATLVVGKWENAIDRVR
LHKELDAGFTTAA

>putative C4-dicarboxylate transport protein [putative transporter]
ATGAGCGAGACCGTCACGATAGACGCGCCGCCCGCGGCGCCCAAACCCCCTACCCCGTTC
TACCGGGACCTGGCCGTCCAGGTCCTCGCAGGCGTCGCGCTGGGCGTCCTGGTGGGCAAC
TACGCCCCCTCTGTCGGGCGGCACACGCAGTTCCTCGGCGACATCTTCATCCACCTGATC
CAGATGGTCGTCGGGCTGATCATCTTCTGCACGGTGACCCACGGCATCGCGAGCGTGCGG
GACCTGGGGAAGGTCGGCCGGATCGCGGTCCGGGCGCTGGTGTACTTCGAGGTGATCACC
ACCGCGGCCCTGATCATCGGGCTGGTCGTGGTCAACGTGCTGCACCCGGGCTCGGGAGTG
CACATCGACCTGTCGCACAACGGCGGGGCCGGCACCGCGCCGGCCAAGTCCGAGGGCTTC
GACGACTTCCTGGTCGGCCTGGTCCCCACCTCGGCGGTGCAGGCGTTCGCCCAGGGCGAC
ATCCTCCAGGTGCTGGTGTTCTCGGTGCTGTTCGCCTGCGGACTGGCCTCGCTGGGCGAG
CGGAGCGCGCCGGTGCTGGAACTGGTCGGCACCGTCCAGAAGACGCTGTTCTGGATCATC
GGCAAGGTGATGCGGATCGCGCCCCTGGCGGCCTTCGGCGCCATCGGCTACACCGTCTCC
AAGTACGGCCTGGGCACGCTCACTTCGCTGGGCAAGCTGATCGGCGTCTTCTACCTGATC
GTGCTCCTCTTCTTCCTCCTCGTCCTGTGGCCGGTCTCCCGGTACGCCGGGGTCAGCCTG
CTGAAGGTGCTGCGCTACTTCCGCGCGGAACTGCTGCTGGTGCTGGGCACCAGCTCGTCG
GAGAGCGTCTTCCCGCAGCTCACGTCGAAGCTGGAGCGGCTGGGCGTCGAGCAGTCGGTG
GTCGGCCTGGTGCTGCCGACCGCGTACAGCTTCAACCACGACGGCACGTGCCTGTACTTC
GCCTCGGTGAGCGTCTTCCTCGCCCAGGCCACCGGTACCGACCTGGGCTGGCAGGCGCAG
ATCGGCCTGCTGCTCATCCTGCTGCTCACGTCCAAGGGCGGGGCGGGGGTCTCCGGCTCG
GCGATCGCCGTGCTGGCGCTGACGCTCAGCTCCACGCACAAGATCCCCGTGGACAGCATC
GCGCTGGTGCTGGGCATCCACAGCATCCTGTCGGCCGCGTTCGTGTTCACCAACATCGCG
GGGAACTGCGTGGCCACGCTGGTGGTCGGCAAGTGGGAGAACGCCATCGACCGGGTCCGG
CTGCACAAGGAACTCGACGCCGGGTTCACGACCGCCGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001991 Sodium:dicarboxylate symporter (Family) PR00173 EDTRNSPORT PF00375 SDF
SignalP	Eukaryota
TMHMM	Transmembrane 8

Rever_00040 Rever_00060