Rever_00160 Rever_00160   Rever_00180 Rever_00180

Rever_00170 : CDS information

close this sectionLocation

Organism
StrainSN-593
Entry nameReveromycin
Contig
Start / Stop / Direction45,877 / 44,684 / - [in whole cluster]
45,877 / 44,684 / - [in contig]
Locationcomplement(44684..45877) [in whole cluster]
complement(44684..45877) [in contig]
TypeCDS
Length1,194 bp (397 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)putative monooxygenase
Gene
Gene (GenBank)revI
EC number
Keyword
  • C-18 hydroxylate
Note
Note (GenBank)
Reference
ACC
PmId
[21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011)
[25258320] Structure-function analyses of cytochrome P450revI involved in reveromycin A biosynthesis and evaluation of the biological activity of its substrate, reveromycin T. (J Biol Chem. , 2014)
comment
[PMID: 21642985]
配列解析によりrevI のfunctionをmonooxygenaseとしている。また、revE〜revN でPost-PKS modificationとしている。実験情報は無し。

---
[PMID: 25258320](2014)
revI の遺伝子・動態・構造調査による特徴づけ。
RevI はreveromycin TのC18-hydroxylationを触媒し、reveromycin Aをもたらす。

close this sectionSequence

selected fasta
>cytochrome P450 [putative monooxygenase]
MNTTYPESLSYPFGPDEGLALSESYTRARDTDGLIRVKLPYGEPAWLATRYEDARLVLGD
ARFSRALSEEHDPPRARKFNAQAKSMFNMDAPDHTRLRRLISKAFTIRRVEELRPKVHDL
AHRLIDDMLAKGEPADLVADYALPIPTTVICELLGVPFEDREKFGRWTDAILSTSTLNPE
EGRVKRMELVGYIGGIIAARRAQPADDLISGMIEARDVQDKLTEQELLDHCIGLLIAGHE
TTASQIPSFVYALLDQPQHWKRLLDDPELIPSAVEELFRFVPLGSGSAAPRYAREDIEVG
GTLVRAGEPVLVALGAANRDGLRFEDPEEIKLDRPSNHHIGFGHGIHHCLGAPLARLELQ
EALRALLERLPTLKVAGDIAWKSEMMVRGPRSMPVGW
selected fasta
>cytochrome P450 [putative monooxygenase]
TTGAACACGACCTACCCGGAGTCGCTGTCCTACCCGTTCGGCCCTGACGAAGGTCTCGCG
CTGAGCGAGTCGTACACCAGGGCGCGCGACACCGACGGCCTGATCCGCGTCAAGCTGCCC
TACGGAGAACCGGCCTGGCTGGCCACCCGCTACGAGGACGCCCGGCTCGTGCTGGGCGAC
GCGCGCTTCAGCCGCGCGCTGTCCGAGGAGCACGACCCGCCGCGGGCACGGAAGTTCAAC
GCGCAGGCGAAGTCCATGTTCAACATGGACGCGCCCGACCACACCCGGCTGCGCCGGCTG
ATCAGCAAGGCGTTCACCATCCGCCGGGTGGAGGAGCTGCGGCCGAAGGTCCACGACCTG
GCCCACCGGCTCATCGACGACATGCTGGCCAAGGGCGAGCCGGCGGACCTGGTGGCGGAC
TACGCGCTGCCGATCCCGACGACCGTCATCTGCGAGCTGCTCGGCGTCCCCTTCGAGGAC
CGGGAGAAGTTCGGCAGGTGGACCGACGCCATCCTGTCGACCAGCACGCTGAACCCCGAG
GAGGGCCGGGTCAAGCGCATGGAGCTGGTCGGCTACATCGGCGGGATCATCGCGGCCCGC
CGCGCACAGCCGGCCGACGACCTCATCTCGGGCATGATCGAGGCCCGCGACGTGCAGGAC
AAGCTCACCGAGCAGGAGTTGCTGGACCACTGCATCGGCCTGCTCATCGCCGGGCACGAG
ACCACCGCCTCCCAGATCCCCAGCTTCGTCTACGCCCTGCTGGACCAGCCGCAGCACTGG
AAGCGGCTGCTGGACGACCCGGAGCTGATCCCGTCGGCCGTCGAGGAGCTGTTCCGCTTC
GTGCCGCTGGGCAGCGGCTCGGCCGCCCCGCGCTACGCCCGGGAGGACATCGAGGTCGGC
GGGACGCTGGTGCGCGCCGGCGAACCGGTCCTGGTGGCCCTCGGCGCGGCCAACCGCGAC
GGGCTGCGCTTCGAGGACCCCGAGGAGATCAAGCTCGACCGCCCGAGCAACCACCACATC
GGCTTCGGCCACGGCATCCACCACTGCCTCGGCGCCCCGCTGGCCCGGCTGGAGTTGCAG
GAGGCGCTGCGCGCGCTCCTGGAGCGGCTGCCGACCCTCAAGGTGGCGGGCGACATCGCC
TGGAAGAGCGAGATGATGGTCCGCGGTCCGCGGTCCATGCCGGTGGGATGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[237-254] 2.90000036269908e-07 PR00385 [272-283] 2.90000036269908e-07 PR00385 [340-349] 2.90000036269908e-07 PR00385 [349-360] 2.90000036269908e-07 PR00385
PR00385   P450
[42-369] 5.69999999999998e-24 PF00067
PF00067   p450
[8-397] 7.19996395544006e-100 SSF48264
SSF48264   Cytochrome_P450
[12-397] 2.09999999999996e-113 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002397 Cytochrome P450, B-class (Family)
[89-100] 1.20000117458134e-60 PR00359 [136-152] 1.20000117458134e-60 PR00359 [153-168] 1.20000117458134e-60 PR00359 [191-213] 1.20000117458134e-60 PR00359 [272-283] 1.20000117458134e-60 PR00359 [291-318] 1.20000117458134e-60 PR00359 [319-334] 1.20000117458134e-60 PR00359 [340-349] 1.20000117458134e-60 PR00359 [349-360] 1.20000117458134e-60 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[342-351] PS00086
PS00086   CYTOCHROME_P450
SignalP
[1-28] 0.058 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Rever_00160 Rever_00160   Rever_00180 Rever_00180
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