Madu_00060 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	12,362 / 13,396 / + [in whole cluster] 12,362 / 13,396 / + [in contig]
Location	12362..13396 [in whole cluster] 12362..13396 [in contig]
Type	CDS
Length	1,035 bp (344 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	dTDP-1-glucose synthase
Gene
Gene (GenBank)	mdpA1
EC number	2.7.7.24
Keyword	madurose
Note
Note (GenBank)
Reference	ACC B0BLL5 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment [PMID:17918933] Maduropeptin生合成遺伝子クラスターの報告 mdpA1: dTDP-1-glucose synthase Maduropeptin生合成遺伝子クラスターは、遺伝子産物の推定機能よりmdpA1からmdpE2までとしている。このORFについて機能解析はしていない。 mdpA1はD-glucose-1-phosphateから始まる5つの酵素反応で形成されるaminosugarの生合成に関与するとしている。
Related Reference	ACC Q9ZGC8 NITE Lando_00120 PmId [16039521] Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A. (Chem Biol. , 2005) comment Streptomyces cyanogenus_lanG NDP-hexose synthetase homolog landomycin生合成遺伝子 lanZ2の機能確認の際に、lanG, lanZ2をE.coliで発現、精製。 LanGのみがD-glucose-1-phosphateを活性化できた。(data not shown)
Related Reference	ACC Q8GMI0 NITE C1027_00100 PmId [14987060] Biochemical characterization of the SgcA1 alpha-D-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor antibiotic C-1027 biosynthetic pathway and overexpression of sgcA1 in Streptomyces globisporus to improve C-1027 production. (J Nat Prod. , 2004) comment Streptomyces globisporus DNTP-glucose synthase C-1027生合成遺伝子大腸菌にてsgcA1を過剰発現させ、精製し、生化学的(分子量,pH依存性,金属イオン要求性,基質特異性)および酵素学的解析を行った。精製酵素はalpha-d-glucopyranosyl-1-phosphate thymidylyltransferaseとして特徴付けられ、sgcA1の過剰発現株はC-1027の生産量が向上した。

Sequence

>putative glucose-1-phosphate thymidylyltransferase [dTDP-1-glucose synthase]
MKALVLAGGTASRLRPFSNSLPKQLIPLAGRPVLEHVLRGIADIGVRDTAIVVGDTAPAV
MDAIGDGARLGLRVTYVPQARPLGLAHCVTLARPFLGDDDFVMYLGDNLLPGGIAGPARR
FRDRRPSAQLLLRRVADPRAFGVAEVGRDGVVRRLEEKPAHPRTDLAVLGVYFFTSAVHA
AIDACRPSHRGELEITDAIQVLIDRGRTVRAVEHTGHWRDIGRPADVLACNRRLLAELRP
RTDGDLDAATEVTGGPVVIEPGARVVRSRITGPALVGADTVLTDCEIGPDAAIGRDCSLR
GVRLRDAVVLDGTSLSGPADVRAALVSPVAAHSVIGAVASGAPG

>putative glucose-1-phosphate thymidylyltransferase [dTDP-1-glucose synthase]
ATGAAAGCTCTGGTGCTGGCGGGGGGCACCGCCTCCCGGCTCCGCCCCTTCAGCAATTCG
CTTCCCAAGCAGCTCATCCCGCTCGCCGGACGCCCGGTCCTGGAACACGTCCTGCGCGGC
ATCGCCGACATCGGCGTCCGGGACACCGCCATCGTGGTGGGCGACACCGCCCCGGCCGTC
ATGGACGCGATCGGCGACGGCGCCCGCCTCGGGCTCCGGGTGACCTACGTCCCGCAGGCC
CGGCCGCTGGGCCTGGCGCACTGCGTCACGCTGGCCCGCCCCTTCCTCGGCGACGACGAC
TTCGTCATGTACCTCGGCGACAACCTGCTGCCGGGCGGCATCGCCGGCCCGGCGCGGCGC
TTCCGCGACCGGCGCCCCAGCGCGCAGCTCCTGCTGCGCCGGGTCGCCGACCCGCGCGCC
TTCGGGGTCGCCGAGGTCGGACGGGACGGCGTCGTCCGGCGGCTCGAGGAGAAGCCCGCG
CACCCGCGCACCGACCTCGCCGTCCTCGGCGTCTACTTCTTCACCTCCGCCGTGCACGCG
GCGATCGACGCCTGCCGCCCGAGCCACCGCGGCGAGCTGGAGATCACCGACGCGATCCAG
GTGCTGATCGACCGGGGCCGCACCGTCCGCGCCGTCGAGCACACCGGCCACTGGCGCGAC
ATCGGGCGGCCCGCGGACGTCCTGGCGTGCAACCGCCGCCTCCTCGCCGAACTGCGGCCC
CGCACGGACGGCGACCTCGACGCGGCCACCGAGGTGACCGGCGGCCCCGTCGTCATCGAA
CCGGGCGCCCGGGTGGTCCGGTCGCGGATCACCGGCCCGGCCCTCGTGGGGGCGGACACC
GTCCTCACCGACTGCGAGATCGGCCCGGACGCCGCCATCGGCCGCGACTGCTCGCTGCGC
GGCGTGCGGCTCCGCGACGCGGTCGTCCTGGACGGCACGTCCCTGTCCGGCCCCGCCGAC
GTCCGCGCCGCCCTCGTCAGTCCGGTCGCCGCGCACAGCGTGATCGGCGCGGTCGCGTCC
GGCGCCCCCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	No significant hit
TMHMM	No significant hit

Madu_00050 Madu_00070