Madu_00260 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	41,116 / 41,619 / + [in whole cluster] 41,116 / 41,619 / + [in contig]
Location	41116..41619 [in whole cluster] 41116..41619 [in contig]
Type	CDS
Length	504 bp (167 aa)

Annotation

Category	3.4 other modification
Product	maduropeptin apoprotein
Product (GenBank)	binding protein
Gene
Gene (GenBank)	mdpA
EC number
Keyword
Note
Note (GenBank)
Reference	ACC B0BLN5 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 mdpA: Apo-Protein maduropeptin cluster内に既知apo-proteinと相同性を示す配列はなかった。 maduropeptin chromoprotein complexを単離、精製したapo-proteinのN末配列を解析し、mdpAがApo-Proteinであることを確かめた。既知DBに相同性を示さない新しいenediyne binding proteinであった。

Sequence

>maduropeptin apoprotein [binding protein]
MQMPKLGRRTALLAAAATMTTGLFAAAGAAPAMADTVTVNYDCVGYPSDIAVTIDAPATA
GVGDTATFEVSSVVTTGAPQDLPAGEVVTDMAIVVGGAQSGTITASGLTNEATPAGQPVN
MVGGEAQITFTSAGSVTFTPSTTTQVDNGAQCTVVGEAPVGATTVVS

>maduropeptin apoprotein [binding protein]
GTGCAAATGCCCAAACTGGGCAGGCGGACCGCGCTGCTGGCGGCGGCCGCCACCATGACC
ACCGGACTGTTCGCCGCGGCGGGCGCCGCGCCCGCCATGGCGGACACCGTCACGGTCAAC
TACGACTGCGTCGGCTACCCGTCGGACATCGCCGTGACCATCGACGCGCCGGCCACGGCC
GGCGTGGGCGACACCGCCACCTTCGAGGTCAGCTCCGTGGTGACCACCGGCGCCCCGCAG
GACCTCCCCGCCGGGGAGGTCGTGACCGACATGGCGATCGTGGTGGGCGGTGCCCAGTCC
GGCACCATCACCGCGAGCGGCCTGACGAACGAGGCCACCCCCGCCGGCCAGCCCGTGAAC
ATGGTCGGCGGCGAGGCCCAGATCACGTTCACCTCGGCCGGCTCCGTCACCTTCACCCCG
TCCACCACCACCCAGGTCGACAACGGCGCGCAGTGCACCGTCGTCGGCGAGGCCCCGGTC
GGCGCCACGACGGTGGTCTCCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006311 Twin-arginine translocation pathway, signal sequence (Conserved_site) PS51318 TAT
SignalP	Bacteria, Gram-positive Eukaryota Bacteria, Gram-negative
TMHMM	Transmembrane 1

Madu_00250 Madu_00270