Calm_00420 Calm_00420   Calm_00440 Calm_00440

Calm_00430 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction52,950 / 52,213 / - [in whole cluster]
52,950 / 52,213 / - [in contig]
Locationcomplement(52213..52950) [in whole cluster]
complement(52213..52950) [in contig]
TypeCDS
Length738 bp (245 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-sugar N-methyltransferase
Product (GenBank)CalS10
Gene
Gene (GenBank)calS10
EC number2.1.1.-
Keyword
  • aminodideoxypentose
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
[19058170] Natural-product sugar biosynthesis and enzymatic glycodiversification. (Angew Chem Int Ed Engl. , 2008)
comment
[PMID:12183629]
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにexpected production of four unusual activated nucleotide sugars(S1 to S14)として示されているのみで、本文中特に触れていない。


[PMID:19058170]
天然糖の生合成に関する総論

この論文中で、UDP-alpha-D-glucoseから進む2 step(calS8,calS9)はdTDP-sugar pathwayでは前例のない経路であり、その後の4 step(calS14, calS12, calS13, calS10)はdTDP-sugar pathwayでは共通して保存されている経路であるがUDP-sugar生合成にとってはuniqueであると記述している。
Related Reference
ACC
Q9ZGH6
NITE
Pikro_00100
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002)
comment
Streptomyces venezuelae_desVI
N,N-dimethyltransferase

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[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesVI: N,N-Dimethyltransferase

desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。

desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。

---
[PMID: 12119032](2002)abstract
N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。
TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

close this sectionSequence

selected fasta
>putative NDP-sugar N-methyltransferase [CalS10]
MFGPEHAEVYEAAYRGRGKSWHDEAADVADRIRAARPDAARLLDVGCGTGAHLETFATRF
PHVEGLELAPAMLALARHRLPGVRLHAGDMRTFDLGVTFDAVTCLFTAVNFLGTVAEMRA
AVAAMSAHLAPGGVLVLEPWWFPERFIDGYVGGDLVREEGRTVARVSRSTRQGRVTRMEE
RWLVGDAAGIREFSQVGLLTMFTREEYDAAFAAAGCESAYVEGWLTGRGLFVATRTGGHA
TPTMV
selected fasta
>putative NDP-sugar N-methyltransferase [CalS10]
ATGTTCGGACCGGAGCACGCCGAGGTGTACGAGGCCGCCTACCGCGGCCGCGGCAAGAGC
TGGCACGACGAGGCGGCGGACGTGGCCGACCGGATCCGGGCCGCCCGCCCCGACGCCGCC
CGGCTGCTCGACGTCGGCTGCGGCACCGGCGCGCACCTCGAGACCTTCGCGACCCGCTTC
CCCCACGTGGAGGGGCTCGAACTGGCCCCGGCGATGCTGGCGCTCGCCCGACACCGGCTG
CCCGGGGTGCGCCTGCACGCCGGGGACATGCGGACGTTCGACCTTGGCGTCACGTTCGAC
GCGGTGACCTGCCTGTTCACCGCGGTCAACTTCCTCGGCACGGTGGCCGAGATGCGGGCG
GCCGTGGCCGCGATGTCGGCCCACCTGGCGCCGGGCGGCGTGCTGGTGCTCGAACCGTGG
TGGTTCCCGGAGCGGTTCATCGACGGGTACGTCGGCGGCGACCTGGTGCGCGAGGAGGGC
CGCACGGTGGCGCGGGTGTCGCGGTCCACCCGGCAGGGACGGGTGACGCGGATGGAGGAG
CGCTGGCTCGTCGGCGACGCCGCCGGGATCCGGGAGTTCAGCCAGGTCGGCCTGCTCACC
ATGTTCACCCGCGAGGAGTACGACGCGGCGTTCGCCGCTGCCGGCTGCGAGTCCGCGTAC
GTCGAGGGCTGGCTGACCGGCCGGGGCCTTTTCGTGGCGACGCGTACCGGTGGACACGCC
ACCCCGACAATGGTTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Calm_00420 Calm_00420   Calm_00440 Calm_00440
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