Calm_00480 Calm_00480   Calm_00500 Calm_00500

Calm_00490 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction58,108 / 57,266 / - [in whole cluster]
58,108 / 57,266 / - [in contig]
Locationcomplement(57266..58108) [in whole cluster]
complement(57266..58108) [in contig]
TypeCDS
Length843 bp (280 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative drug resistance ABC transporter permease protein
Product (GenBank)CalT4
Gene
Gene (GenBank)calT4
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
comment
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにて、membrane transport(T1 to T7)と示されているのみ。本文中では特にふれていない。
Related Reference
ACC
O86917
PmId
[9696745] A silent ABC transporter isolated from Streptomyces rochei F20 induces multidrug resistance. (J Bacteriol. , 1998)
comment
Streptomyces rochei_ORF2
ORF2 protein

Streptomyces rochei F20で、Msr-ORF1とMsr-ORF2は、ABC transporter superfamily のメンバーであるATP-binding proteinとtransmembrane proteinに各々似ていた。msrを破壊しても形質変化は認められず、行った実験条件では発現が検出されず、inducerの発見には至っていない。msrのプロモーター領域をexpressing promoterに入れ替えたプラスミドを作成し、Streptomyces lividans TK21に形質転換させると、薬剤や化学物質(doxorubicin, oleandomycin, erythromycin, tetracycline, spiramycin, streptothricin, tobramycin, novomycin, streptomycin, chloramphenicol, phenol, pentachlorophenol, tetrachlorohydroquinone, ethidium bromide)に抵抗性を示した。
ACC
P32011
NITE
Adria_00140
PmId
[1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991)
[9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997)
[15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005)
[19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
comment
Streptomyces peucetius
Daunorubicin/doxorubicin resistance ABC transporter permease protein drrB

[PMID:1924314]
DrrAは排出ABC transporterのATP結合タンパク質。Mg2+依存的にATP (or GTP) を結合。
doxorubicin(DXR) or daunorubicin(DNR)の添加で活性が上昇。
DrrBはABC-2 type transporterといわれているもの。

--
さらにいくつかの続報がでており、DrrABはATP依存的なDXRとDNRの排出ポンプとされている(PMID:15709779)。
上記著者らでの最新の続報は[PMID:19063901]

--
違うグループでも報告あり。
上記既報をrefとして採用している。

[PMID:20158521]
drrAB(-)mutantはDNRに高い感受性あり。
このmutantはwildと比べて、薬剤産生が10倍減。
key regulator dnrI and polyketide synthase gene dpsAの発現が激減。
regulatory genes dnrO and dnrNの発現は増。
これらの結果から細胞内DNR濃度に基づくFeedback regulationを提唱。

close this sectionSequence

selected fasta
>putative drug resistance ABC transporter permease protein [CalT4]
MTTPSTEVRPLPAEIFSRSVAGAERPPRPGPLFAVRTFAWRNLIKLRYVQDHLGTAVVFP
IILTLVFTYLLGGAIAGSPREYLQFFLPGVIVLSLVSSSMMSALTLNRDIATGMFDRVRS
TPIWQPAVLVGAMAGDAVRYALTSIVPLSLGLLLGFRPDGGLSGVVLALLYLQLFTFSVA
WLWMLFAVLIPQPTAAAGVVNLLQFVLLFGSNILAPSQTMPGWLEAVVKLNPVTHAATAT
RGLMHGTVTSGEMGAGLLTCAVLIVLLAPPTIWLYSRKQR
selected fasta
>putative drug resistance ABC transporter permease protein [CalT4]
ATGACGACACCCAGCACCGAGGTGCGGCCGCTGCCGGCCGAGATCTTCAGCCGATCGGTG
GCCGGCGCGGAACGGCCGCCACGCCCCGGCCCGCTGTTCGCCGTCCGCACCTTCGCCTGG
CGGAACCTGATCAAGCTCCGGTACGTGCAGGACCACCTGGGCACCGCGGTGGTCTTCCCG
ATCATCCTGACGCTGGTCTTCACCTATCTGCTCGGCGGCGCGATCGCCGGCTCGCCCCGG
GAGTACCTGCAGTTCTTCCTTCCCGGCGTGATCGTCCTCTCGCTCGTGTCGTCGAGCATG
ATGAGCGCCCTGACGCTGAACCGGGACATCGCCACCGGCATGTTCGACCGGGTCCGCAGC
ACGCCCATCTGGCAGCCCGCGGTACTGGTCGGGGCGATGGCCGGCGACGCCGTCCGGTAC
GCCCTGACCTCGATCGTGCCGCTGTCGCTCGGCCTGCTGCTCGGCTTCCGGCCGGACGGC
GGCCTGTCCGGGGTGGTGCTCGCCCTGCTCTACCTGCAGCTGTTCACCTTCAGCGTCGCC
TGGCTGTGGATGCTGTTCGCGGTGCTGATCCCGCAGCCGACCGCCGCCGCCGGCGTGGTG
AACCTCCTGCAGTTCGTGCTCCTCTTCGGCAGCAACATCCTGGCGCCGTCGCAGACGATG
CCGGGCTGGCTGGAGGCGGTGGTCAAGTTGAACCCCGTCACCCACGCCGCGACCGCCACC
CGCGGGCTGATGCACGGCACGGTGACCTCGGGGGAGATGGGCGCCGGCCTGCTGACCTGC
GCCGTGCTCATCGTGCTGCTCGCCCCGCCCACGATCTGGCTCTACAGCCGCAAGCAGCGC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000412 ABC-2 (Family)
[51-278] PS51012
PS51012   ABC_TM2
IPR013525 ABC-2 type transporter (Domain)
[36-244] 7.5e-23 PF01061
PF01061   ABC2_membrane
SignalP No significant hit
TMHMM
[53-75] Transmembrane (i-o) [85-107] Transmembrane (o-i) [128-150] Transmembrane (i-o) [165-187] Transmembrane (o-i) [194-216] Transmembrane (i-o) [253-275] Transmembrane (o-i)
Transmembrane 6   
Calm_00480 Calm_00480   Calm_00500 Calm_00500
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