Calm_00490 Calm_00490   Calm_00510 Calm_00510

Calm_00500 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction59,091 / 58,105 / - [in whole cluster]
59,091 / 58,105 / - [in contig]
Locationcomplement(58105..59091) [in whole cluster]
complement(58105..59091) [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative drug resistance ABC transporter ATP-binding protein
Product (GenBank)CalT5
Gene
Gene (GenBank)calT5
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
comment
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにて、membrane transport(T1 to T7)と示されているのみ。本文中では特にふれていない。
Related Reference
ACC
P32010
NITE
Adria_00130
PmId
[1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991)
[9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997)
[15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005)
[19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
[25721665] Characterization of a novel domain 'GATE' in the ABC protein DrrA and its role in drug efflux by the DrrAB complex. (Biochem Biophys Res Commun. , 2015)
comment
Streptomyces peucetius
Daunorubicin/doxorubicin resistance ATP-binding protein drrA

[PMID:1924314]
DrrAは排出ABC transporterのATP結合タンパク質。Mg2+依存的にATP (or GTP) を結合。
doxorubicin or daunorubicinの添加で活性が上昇。
DrrBはABC-2 type transporterといわれているもの。

--
さらにいくつかの続報がでており、DrrABはATP依存的なdoxorubicinとdaunorubicinの排出ポンプとされている(PMID:15709779など)。
上記著者らでの最新の続報は、[PMID:19063901]

--
違うグループでも報告あり。
上記既報をrefとして採用している。

[PMID:20158521]
drrAB(-)mutantはDNRに高い感受性あり。
このmutantはwildと比べて、薬剤産生が10倍減。
key regulator dnrI and polyketide synthase gene dpsAの発現が激減。
regulatory genes dnrO and dnrNの発現は増。
これらの結果から細胞内DNR濃度に基づくFeedback regulationを提唱。

close this sectionSequence

selected fasta
>putative drug resistance ABC transporter ATP-binding protein [CalT5]
MTTNPALAIETRDLVKVFGQTRAVDGLDLVVRAGTIHGVLGPNGAGKTTAIKMLATLMRP
TSGTASVLGHDVVREAAEVRRRIGLTGQTMSVDEDMTGVQNLILAGRLQGLRHASAAARA
EQLMEAFDLTEVGGRLVKTFSGGQRRRIDVAASMVVTPELLFLDEPTTGLDPRSRSEVWE
MIRALVRDGGTVLLTTQYLDEADHLADELTLIDHGRIVAQGTPPELKASRAAGVLDVRLR
DPERRADAGALLAKAVGAAADLDSDPARLSVRVTDPDRAALALGELARAGIHVDDFTLGQ
PSLDTVFLALTGHSTVDASEEEEAEVRA
selected fasta
>putative drug resistance ABC transporter ATP-binding protein [CalT5]
ATGACAACCAATCCGGCCTTGGCCATCGAGACCCGCGATCTGGTGAAGGTCTTCGGCCAG
ACGCGCGCGGTCGACGGACTGGACCTGGTGGTGCGGGCCGGGACGATCCACGGGGTGCTG
GGCCCGAACGGCGCCGGCAAGACGACGGCCATCAAGATGCTCGCCACGCTGATGCGACCC
ACCTCCGGCACCGCGTCCGTGCTGGGGCACGACGTGGTCCGCGAGGCCGCCGAGGTCCGG
CGCCGCATCGGCCTCACCGGCCAGACCATGTCCGTCGACGAGGACATGACCGGCGTGCAG
AACCTGATCCTCGCCGGCCGCCTGCAGGGTCTGCGGCACGCGTCCGCGGCCGCGCGGGCG
GAGCAGTTGATGGAGGCGTTCGACCTCACCGAGGTCGGCGGCCGGCTGGTGAAGACCTTC
TCCGGCGGGCAGCGGCGGCGCATCGACGTGGCCGCGAGCATGGTGGTCACCCCCGAGCTG
CTGTTCCTCGACGAGCCGACCACCGGCCTCGACCCGCGCAGCCGCAGCGAGGTCTGGGAG
ATGATCCGGGCGCTGGTCCGGGACGGGGGCACCGTCCTGCTGACCACGCAGTACCTCGAC
GAGGCGGACCACCTCGCCGACGAGCTGACGCTCATCGACCACGGCCGCATCGTGGCGCAG
GGCACCCCGCCGGAGCTGAAGGCGAGCCGCGCCGCCGGCGTGCTCGACGTGCGGCTGCGT
GACCCCGAGCGCCGGGCCGACGCGGGCGCCCTGCTCGCCAAGGCCGTCGGCGCCGCCGCC
GACCTCGACTCCGATCCGGCGCGGCTGTCGGTGCGGGTGACCGACCCCGACCGGGCGGCG
CTGGCCCTGGGCGAGCTGGCGCGGGCCGGCATCCACGTCGACGACTTCACGCTCGGCCAG
CCCTCGCTCGACACGGTGTTCCTCGCCCTCACCGGTCACTCGACGGTCGACGCCAGCGAA
GAAGAGGAAGCAGAGGTACGGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
[9-239] PS50893
PS50893   ABC_TRANSPORTER_2
[48-168] 4.1e-16 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[33-225] 1.20000117458134e-10 SM00382
SM00382   AAA
IPR005894 Daunorubicin resistance ABC transporter ATP-binding subunit (Family)
[16-312] 2.20000000000002e-107 TIGR01188
TIGR01188   DrrA
IPR017871 ABC transporter, conserved site (Conserved_site)
[140-154] PS00211
PS00211   ABC_TRANSPORTER_1
SignalP No significant hit
TMHMM No significant hit
Calm_00490 Calm_00490   Calm_00510 Calm_00510
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