Calm_00620 Calm_00620   Calm_00640 Calm_00640

Calm_00630 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction75,152 / 76,186 / + [in whole cluster]
75,152 / 76,186 / + [in contig]
Location75152..76186 [in whole cluster]
75152..76186 [in contig]
TypeCDS
Length1,035 bp (344 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative acyltransferase
Product (GenBank)CalO4
Gene
Gene (GenBank)calO4
EC number
Keyword
  • orsellinic acid moiety
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
[21987796] Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity. (Proc Natl Acad Sci U S A. , 2011)
comment
[PMID:12183629]
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにて、orsellinic acid 生合成遺伝子と示されているのみ。本文中では特にふれていない。


[PMID:21987796]
glycosyltransferaseの解析論文。
CalO4について本文には出ていないが、Fig.1 にて糖へのorsellinic acid moietyの付着に関与すると記載されている。
Related Reference
ACC
Q0R4P5
NITE
Chth_00090
PmId
[19266533] Dissection of two acyl-transfer reactions centered on acyl-S-carrier protein intermediates for incorporating 5-chloro-6-methyl-O-methylsalicyclic acid into chlorothricin. (Chembiochem. , 2009)
comment
Streptomyces antibioticus
ChlB3
[DoBISCUIT]6-methylsalicylyltransferase

異種性発現、精製したChlB1 ACP domain, ChlB2, ChlB3, ChlB6を使い、ChlB1(PKS)-ACPからChlB2(ACP)への6-methylsalicylyl transferがChlB3によるものであり、ChlB6ではできないことをHPLCで確認している。6-methylsalicylic acid(6-MSA)では活性が検出されないので、ChlB1(PKS)-ACPから6-MSAとして放出されることなく、直接ChlB2(ACP)へ移動される。

close this sectionSequence

selected fasta
>putative acyltransferase [CalO4]
MRTAGTYIRGIGAYLPETVTVEEAVAQGLYPQEDIETHGLGGAAIAGELPAPDMALRAAQ
DALKESELGRGDIDLLLYAAAWHQGPEGWLAHSYIQHYLLGGVPRATEIRQGCNGMFTML
ELAASYLKAAPERKAAMLVAADNYGTPLLDRWRTNLGFILGDAASAVVLSTESGFVELMS
VCSITVPEAEEVHRGGEPMFPPGATLAKELDFGARLFYHITEQTPVLAVLGEAQETMTTV
AEQALAEAGIGTGDLAKVSFMNYSREVVEQRCMAPLGLGMEKSTWDFGRMIGHCGASDHL
LALHHSLRAGEVAAGDHVLWLAMGPGVEFTAAVLRVLDNPYVER
selected fasta
>putative acyltransferase [CalO4]
ATGCGCACCGCCGGAACGTACATCCGTGGGATCGGGGCCTACCTTCCTGAGACCGTCACC
GTCGAGGAAGCCGTCGCCCAGGGCCTGTACCCGCAGGAGGACATCGAGACGCACGGGCTG
GGCGGGGCCGCGATCGCCGGCGAACTGCCCGCGCCGGACATGGCGCTGCGGGCCGCGCAG
GACGCCCTCAAGGAGAGCGAGCTGGGCCGCGGCGACATCGACCTGCTGCTCTACGCGGCC
GCCTGGCACCAGGGCCCCGAGGGCTGGCTGGCGCACTCCTACATCCAGCACTACCTGCTC
GGCGGGGTGCCCCGGGCGACCGAGATCCGGCAGGGCTGCAACGGCATGTTCACCATGCTC
GAACTCGCGGCCAGCTACCTGAAGGCCGCGCCGGAACGCAAGGCGGCGATGCTCGTCGCC
GCCGACAACTACGGCACCCCGCTGCTGGACCGCTGGCGCACCAACCTCGGCTTCATCCTC
GGCGACGCCGCCTCCGCGGTGGTGCTGAGCACCGAGAGCGGCTTCGTCGAGCTGATGTCG
GTCTGCTCCATCACCGTGCCGGAGGCCGAGGAGGTGCACCGCGGCGGCGAGCCGATGTTC
CCGCCCGGCGCGACGCTCGCCAAGGAGCTCGACTTCGGCGCCCGGCTCTTCTACCACATC
ACCGAGCAGACCCCCGTGCTCGCCGTGCTCGGCGAGGCGCAGGAGACGATGACCACCGTC
GCCGAGCAGGCGCTCGCCGAGGCCGGCATCGGCACGGGAGACCTGGCCAAGGTCTCCTTC
ATGAACTACTCCCGCGAGGTGGTCGAGCAGCGCTGCATGGCGCCCCTGGGGCTGGGGATG
GAGAAGTCCACCTGGGACTTCGGGCGGATGATCGGGCACTGCGGCGCCAGCGACCACCTG
CTCGCCCTGCACCACTCGCTGCGGGCCGGTGAGGTCGCCGCCGGCGACCACGTGCTGTGG
CTGGCGATGGGCCCCGGCGTGGAGTTCACCGCCGCCGTCCTGCGCGTACTGGACAACCCC
TACGTCGAGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013747 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (Domain)
[245-335] 3.7e-18 PF08541
PF08541   ACP_syn_III_C
IPR013751 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (Domain)
[108-178] 2e-11 PF08545
PF08545   ACP_syn_III
IPR016038 Thiolase-like, subgroup (Domain)
[3-165] 1.3e-23 G3DSA:3.40.47.10 [166-336] 1e-20 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[8-339] 2.800005042611e-23 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit
Calm_00620 Calm_00620   Calm_00640 Calm_00640
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