Dynm_00140 Dynm_00140   Dynm_00160 Dynm_00160

Dynm_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 53710 (=NBRC 15963)
Entry nameDynemicin
Contig
Start / Stop / Direction22,855 / 23,841 / + [in whole cluster]
22,855 / 23,841 / + [in contig]
Location22855..23841 [in whole cluster]
22855..23841 [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)DynU15
GenedynU15
unbL
Gene (GenBank)
EC number
Keyword
  • enediyne core
Note
Note (GenBank)
  • hypothetical protein; CalU15-like protein
Reference
ACC
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
[12536216] A genomics-guided approach for discovering and expressing cryptic metabolic pathways. (Nat Biotechnol. , 2003)
comment
[PMID:18328078]
dynemicin生合成遺伝子クラスターの報告

dynU15: Unknown conserved protein

dynU15欠損株を作製し、培養液中の産物をHPLCにて分析。dynU15欠損株はdynemicin Aが検出出来なかったことから、dynU15はdynemicin Aに必須であるとしている。

dynU15, dynU14, dynT3, dynE8, dynE7は、minimal enediyne cassetteとして知られている遺伝子と推定している。dynU15, dynU14, dynT3はconserved unknown proteinsだと記述あり。


[PMID:12536216]
warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)の報告

warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)はactinomyceteにおいて全てのenediyne生合成遺伝子座で保存されているが、機能は未知のままである。enediyne warheadのformation, stabilization, or transport に関与しているのではないかと推測している。dynemicinでも並びが保存されている。

close this sectionSequence

selected fasta
>hypothetical protein [DynU15]
MTSTLTRDGVHRPGGWRSRLALSPDLVDFANRGFRTDRPETRYVLERAATAFLDGYNRGL
DTPYGQPTTLDGIPADLRGFAAEGSAMSAVLLDRLSPRRHGRFAAQLRGHPRHDYLLHVG
AGWALAKLDAVSVGALGRLGRLGSENGLLRWLAYDGYGFFKVFFVKDPTLAGWRRHRKPC
DPTCAIRYQGLGRCLWFRDCGDPAALAERISGLPASHRGDVWSGVGLAATYANGVSPDDH
ALLLRLAGNHRPALAQGAAFAAEARRADGTAGPAVDAATRTLTGVSADTAAEWTWQARHG
LDRAGATPADYGEWRARVQRLAARHVAG
selected fasta
>hypothetical protein [DynU15]
ATGACATCGACGCTCACGCGGGACGGGGTGCACCGACCCGGCGGCTGGCGGAGCCGGTTG
GCGCTGAGTCCGGACCTGGTGGACTTCGCCAACCGCGGCTTCCGGACCGACCGCCCTGAG
ACGAGGTACGTCCTGGAACGCGCCGCCACCGCCTTCCTCGACGGCTACAACCGCGGCCTG
GACACGCCGTACGGGCAGCCGACCACACTGGACGGCATCCCGGCTGACCTGCGCGGATTC
GCCGCCGAGGGGTCGGCCATGTCGGCCGTGCTGCTGGACCGGCTGTCGCCCCGGCGGCAC
GGCCGCTTCGCCGCCCAGTTGCGCGGGCACCCGCGCCACGACTACCTGCTGCACGTCGGC
GCGGGCTGGGCGCTGGCCAAGCTGGACGCCGTCTCGGTCGGTGCGCTGGGGCGGCTCGGC
CGCCTCGGATCGGAGAATGGCCTGCTCCGCTGGCTGGCGTACGACGGCTATGGCTTTTTC
AAGGTCTTTTTCGTCAAGGATCCGACCCTCGCGGGCTGGCGCCGGCACCGGAAGCCCTGC
GACCCGACCTGCGCCATCCGCTACCAGGGGCTGGGTCGCTGCCTCTGGTTCCGCGACTGC
GGCGACCCGGCCGCCCTGGCCGAGCGGATCAGCGGCCTGCCGGCCTCGCACCGTGGTGAC
GTGTGGAGCGGCGTCGGCCTCGCCGCCACGTACGCCAACGGGGTTTCCCCGGACGACCAC
GCCCTGCTGCTGCGGCTCGCCGGCAACCACCGGCCCGCCCTCGCCCAGGGCGCCGCGTTC
GCGGCGGAGGCCCGCCGGGCGGACGGCACCGCGGGCCCGGCGGTCGACGCCGCCACCCGG
ACCCTGACCGGGGTGTCCGCCGACACCGCCGCCGAGTGGACCTGGCAGGCCCGGCACGGC
CTGGACCGGGCCGGCGCCACCCCGGCCGACTACGGCGAGTGGCGCGCCCGCGTGCAGCGG
CTCGCCGCCCGGCACGTCGCCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR012964 Protein of unknown function DUF1702 (Family)
[16-322] 5.00000000000001e-102 PF08012
PF08012   DUF1702
SignalP No significant hit
TMHMM No significant hit
Dynm_00140 Dynm_00140   Dynm_00160 Dynm_00160
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