Dynm_00480 Dynm_00480   Dynm_00500 Dynm_00500

Dynm_00490 : CDS information

close this sectionLocation

Organism
StrainATCC 53710 (=NBRC 15963)
Entry nameDynemicin
Contig
Start / Stop / Direction62,762 / 62,268 / - [in whole cluster]
62,762 / 62,268 / - [in contig]
Locationcomplement(62268..62762) [in whole cluster]
complement(62268..62762) [in contig]
TypeCDS
Length495 bp (164 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase iron-sulfur binding subunit
Product (GenBank)putative carbon monoxide dehydrogenase small subunit
Geneorf22
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF36-2
Reference
ACC
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
comment
Dynemicin生合成遺伝子クラスターの報告。

orf22 Molybdenum-containing oxidoreductase-small subunit, (2Fe-2S)-binding subunit
Genbank orf36-2

prokaryotic molybdenum containing hydroxylasesと相同性示すと明記。
orf21 :large subunit
orf22 :small subunit
orf23 :medium subunit

機能解析はされていない。
Related Reference
ACC
P19921
PmId
[7721710] ( , )
[10430865] ( , )
comment
Oligotropha carboxidovorans_coxS
Carbon monoxide dehydrogenase small chain

[PMID:7721710]
coxMSL,orf4のシーケンス解析と転写解析。
carbon-monoxide dehydrogenaseはcoxLMSの三量体2個から成り、次の反応を触媒する。 CO + H2O → CO2 + 2e- + 2H+ Lサブユニットは88.7kDaであり、Mo cofactorを持つ。

[PMID:10430865]
coxS結晶構造解析
ACC
P72223
PmId
[8798497] ( , )
comment
Pseudomonas putida_ qorS
Quinoline 2-oxidoreductase

Quinoline 2-oxidoreductase、qorS large, medium, small3つのsubunitで構成されるoxidoreductaseで、それぞれqorL, qorM, qorSにコードされている。実験で、quinoline degradation活性を確認。 このアミノ酸配列はこの2つと相同性が高いことも判明。 prokaryotic molybdenum containing hydroxylases eukaryotic xanthine dehydrogenases
ACC
P19915
PmId
[10966817] ( , )
[11076018] ( , )
comment
Hydrogenophaga pseudoflava_cutS
Carbon monoxide dehydrogenase small chain
EC 1.2.99.2

特徴付け、結晶構造解析がされている。Carbon monoxide dehydrogenases (CO-DH) はmolybdopterin flavin adenine dinucleotide (FAD), two different iron-sulfur clustersを含む3つのサブユニット(cutM-cutS-cutL)から構成されており、carboxydobacteriaの二酸化炭素への COの酸化に反応する酵素。

[PMID:11076018]
CO-DHに関する総説。

close this sectionSequence

selected fasta
>putative oxidoreductase iron-sulfur binding subunit [putative carbon monoxide dehydrogenase small subunit]
MESVELLVNGVRREGRVEPRRTLADFLREDLGLTGTHVGCEQGACGACTVLLDGEPVYAC
LLFAVQARGHEITTVEGLAAPGELDPMQDAFRRCHALQCGFCTPGMLLAAHGLLADDPDP
DDAGIRIALAGNICRCTGYQSIVDAVRLAAAERRRSAAPDGAAR
selected fasta
>putative oxidoreductase iron-sulfur binding subunit [putative carbon monoxide dehydrogenase small subunit]
ATGGAGTCCGTCGAGCTGCTGGTGAACGGGGTACGCCGGGAGGGGCGGGTCGAGCCGCGC
CGGACGCTTGCCGACTTCCTCCGCGAGGATCTCGGGCTGACCGGCACGCACGTCGGCTGC
GAGCAGGGCGCCTGCGGCGCGTGCACGGTGCTCCTCGACGGCGAGCCGGTCTACGCCTGC
CTGCTCTTCGCCGTGCAGGCGCGGGGCCACGAGATCACCACGGTCGAGGGGCTGGCCGCC
CCGGGCGAGCTGGATCCCATGCAGGACGCCTTCCGCCGGTGCCACGCCCTCCAGTGCGGT
TTCTGCACCCCGGGCATGCTGCTGGCCGCGCACGGGCTGCTCGCCGACGACCCGGACCCG
GACGACGCGGGCATCCGGATCGCGCTCGCCGGCAACATCTGCCGCTGCACCGGCTACCAG
AGCATCGTGGACGCGGTCCGGCTCGCCGCGGCGGAGCGGCGCCGGTCGGCCGCGCCGGAC
GGAGCGGCGCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001041 2Fe-2S ferredoxin-type domain (Domain)
[1-79] 5.69999708783953e-28 SSF54292
SSF54292   Ferredoxin
[2-78] PS51085
PS51085   2FE2S_FER_2
[8-56] 2.8e-07 PF00111
PF00111   Fer2
IPR002888 [2Fe-2S]-binding (Domain)
[74-154] 7.0999832511256e-33 SSF47741
SSF47741   2Fe-2S_bind
[74-147] 2.1e-30 PF01799
PF01799   Fer2_2
[76-155] 4.5e-32 G3DSA:1.10.150.120
G3DSA:1.10.150.120   2Fe-2S-bd
IPR006058 2Fe-2S ferredoxin, iron-sulphur binding site (Binding_site)
[40-48] PS00197
PS00197   2FE2S_FER_1
IPR012675 Beta-grasp domain (Domain)
[2-75] 5.90000000000001e-29 G3DSA:3.10.20.30
G3DSA:3.10.20.30   Ferredoxin_fold
SignalP No significant hit
TMHMM No significant hit
Dynm_00480 Dynm_00480   Dynm_00500 Dynm_00500
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