Salino3_00350 : CDS information

Location

Organism	Streptomyces albus
Strain	XM211
Entry name	Salinomycin
Contig	JN033543
Start / Stop / Direction	108,927 / 108,679 / - [in whole cluster] 108,927 / 108,679 / - [in contig]
Location	complement(108679..108927) [in whole cluster] complement(108679..108927) [in contig]
Type	CDS
Length	249 bp (82 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	Orf14
Reference	ACC H6D591 PmId [22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012) comment polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いて、Streptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。 Orf14 (82aa) : Hypothetical protein orf14-orf17を削除すると、salinomycin産生はwildの45%に低下した。この結果はSARP family transcriptional regulator gene orf15が削除されたためと考えられている。
Related Reference	ACC H1ZZV7 NITE Salino_01150 PmId [22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012) comment 配列は[H6D591]と全く同じ。株違い。このORFの周辺は遺伝子編成が少し異なる。 --- [PMID: 22076845] monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。 orf6 (73aa) : unknown 詳細記載なし。

Sequence

>hypothetical protein [hypothetical protein]
MFPVVKVVPVSEFAFGVDLTEGEMRRRAAVVEALGSDWDPVAVLEGERAAHDLLYSGLDA
EQQKTYELLVAAGVLEDRQARP

>hypothetical protein [hypothetical protein]
GTGTTCCCGGTAGTGAAGGTGGTCCCCGTGAGCGAGTTCGCGTTCGGCGTCGACCTGACG
GAGGGCGAGATGCGGCGCCGTGCCGCGGTCGTCGAGGCGCTCGGCTCCGACTGGGACCCG
GTGGCCGTACTGGAGGGCGAGCGCGCCGCCCACGATCTGCTCTACTCCGGTCTCGACGCG
GAGCAGCAGAAGACCTACGAGCTGCTCGTGGCCGCGGGTGTCCTGGAGGACCGGCAGGCC
CGGCCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Salino3_00340 Salino3_00360