Balhi_00020 Balhi_00020   Balhi_00040 Balhi_00040

Balhi_00030 : CDS information

close this sectionLocation

Organism
StrainDSM 5908
Entry nameBalhimycin
Contig
Start / Stop / Direction1,537 / 2,157 / + [in whole cluster]
1,537 / 2,157 / + [in contig]
Location1537..2157 [in whole cluster]
1537..2157 [in contig]
TypeCDS
Length621 bp (206 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
ProductD-alanyl-D-alanine carboxypeptidase
DD-carboxypeptidase
Product (GenBank)putative VanY-type carboxypeptidase
Gene
Gene (GenBank)vanY
EC number3.4.16.4
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17693715] The border sequence of the balhimycin biosynthesis gene cluster from Amycolatopsis balhimycina contains bbr, encoding a StrR-like pathway-specific regulator. (J Mol Microbiol Biotechnol. , 2007)
[21690280] Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. (Antimicrob Agents Chemother. , 2011)
comment
[PMID: 17693715](2007)
vancomycinにとても似たグリコペプチド系抗生物質balhimycinの生合成gene clusterの追加報告。
新たにVanS, VanR, VanY, Bbr, Pdh, Tba, Dahpを同定している。

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[PMID: 21690280](2011)
vancomycin様グリコペプチドであるbalhimycinの生合成gene clusterにあるaccessory resistance gene orthologs vanY, vnlR, vnlSの役割を調査。

VanYを過剰発現したE.coliの細胞抽出液は、diacetyl-Lys-D-Ala-D-Alaに対してかなりのDD-carboxypeptidase活性を示すが、diacetyl-Lys-D-Ala-D-Lacでは検出されなかった。よってVanYはDD-carboxypeptidase活性を持ちpeptidoglycan前駆体から末端のD-Alaを切断するが、DD-carboxyesterase活性を持たない。

vanHAX genesを誘導できるStreptomyces coelicolorでのvanYの異種性発現は、balhimycin耐性を拡大した。regulatory two-component system VanRSがないためvanHAX genesが誘導されないglycopeptide感受性mutant S.coelicolor J3201においてvanYを異種性発現しても、balhimycin耐性は与えられなかった。よってS.coelicolorにおいて、vanY単独では耐性を与えることはできず、機能的なvanHAX genesを必要とする。

close this sectionSequence

selected fasta
>D-alanyl-D-alanine carboxypeptidase [putative VanY-type carboxypeptidase]
MTYRESARTTTRRIPGAVVPVARRIRGVLLAGLRAVGTRIARSPGRPVRPQDRAGLGKTH
GAVPAGVTVFDDDVPAVTRLDPALLSALRRAATAAADGGVELCVNSGWRSPEYQSRLLRE
AVAKYGSAAAAARWVATPETSIHVAGKAVDIGPPASASWLSEHGADYGLCRVYRNEPWHF
ELRPEAIEHGCPPLYADPSHDPRLRR
selected fasta
>D-alanyl-D-alanine carboxypeptidase [putative VanY-type carboxypeptidase]
ATGACCTACCGCGAGTCGGCCCGGACGACGACCCGCCGGATTCCCGGCGCCGTCGTGCCG
GTGGCCCGCCGGATTCGCGGGGTCCTTCTCGCCGGCCTGCGCGCCGTCGGCACGAGGATT
GCCCGGTCGCCCGGTCGCCCGGTCCGCCCCCAGGACCGTGCCGGCCTCGGCAAGACCCAC
GGTGCCGTCCCCGCCGGGGTGACGGTCTTCGACGACGACGTCCCGGCTGTGACTCGCCTC
GACCCGGCGCTTCTGAGTGCACTGCGCCGGGCCGCGACCGCGGCCGCCGACGGCGGGGTC
GAACTGTGCGTGAACAGCGGCTGGCGGTCTCCGGAATACCAGAGCCGGCTTCTTCGCGAG
GCGGTGGCGAAATACGGGTCGGCGGCGGCGGCCGCCCGGTGGGTGGCCACCCCGGAGACG
TCGATCCACGTGGCGGGGAAGGCGGTCGACATCGGGCCACCCGCGTCCGCGTCGTGGTTG
TCCGAGCACGGCGCCGATTACGGGTTGTGCCGCGTCTACCGCAACGAACCCTGGCACTTC
GAATTGCGTCCCGAAGCGATCGAGCACGGCTGCCCGCCCCTGTATGCCGACCCGAGTCAC
GACCCGCGGCTGCGCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003709 Peptidase M15B/M15C (Domain)
[79-183] 1.69999999999999e-17 PF02557
PF02557   VanY
IPR009045 Hedgehog signalling/DD-peptidase zinc-binding domain (Domain)
[76-197] 5.59999989417023e-07 SSF55166
SSF55166   Hedgehog_sig_N
[72-156] 6.69999999999999e-05 G3DSA:3.30.1380.10
G3DSA:3.30.1380.10   Hedgehog/DD-pept_Zn-bd
SignalP No significant hit
TMHMM No significant hit
Balhi_00020 Balhi_00020   Balhi_00040 Balhi_00040
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