A4092_00110 : CDS information

Location

Organism	Nonomuraea sp.
Strain	ATCC 39727
Entry name	A40926
Contig	AJ561198
Start / Stop / Direction	13,385 / 14,494 / + [in whole cluster] 13,385 / 14,494 / + [in contig]
Location	13385..14494 [in whole cluster] 13385..14494 [in contig]
Type	CDS
Length	1,110 bp (369 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative prephenate dehydrogenase
Product (GenBank)	putative prephenate dehydrogenase
Gene	dbv5 pdh
Gene (GenBank)	dbv5
EC number	1.3.1.-
Keyword	Hpg
Note
Note (GenBank)
Reference	ACC Q7WZ86 PmId [12837387] The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. (Chem Biol. , 2003) [17873036] Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. (J Bacteriol. , 2007) comment [PMID: 12837387](2003) Nonomuraea sp. ATCC39727由来A40926生合成gene clusterの同定論文。 A40926は半合成グリコペプチドdalbavancin (BI397 or MDL 62,397)の前駆体。 dbv ORF5: prephenate dehydrogenase p-hydroxyphenylglycine (HPG)合成のためのp-hydroxyphenylpyruvate供給を担うと考えられている。配列解析のみ。 --- [PMID: 17873036](2007) RT-PCRとその産物のシークエンスで、dbv5-dbv7が同時転写されることを確認。リン酸による遺伝子発現への影響がRT-PCR と real-time RT-PCRで調査されている。dbv6は影響を受けない。
Related Reference	ACC Q04983 PmId [7916685] ( , ) comment BLAST id33% Zymomonas mobilis subsp. mobilis_tyrC Protein TyrC Alternative name(s): Arogenate dehydrogenase(ADH,EC 1.3.1.43) Prephenate dehydrogenase(PDH,EC 1.3.1.12) Cyclohexadienyl dehydrogenase E.coliでtyrCを発現させ、精製し、分子量や活性など見ている。ADHとPDHの活性の割合は3：1で、これらの活性は分離できない。N末にNAD+ binding domainあり。 B. subtilisやS. cerevisiaeのPDH、E.coliやE.herbicolaのbifunctional T-proteinsとはアミノ酸配列の相同性は低い。 tyrosineによるfeedback阻害は受けない。
Family/Domain	FD IPR003099 comment [IPR003099] Prephenate dehydrogenase (Domain) Molecular Function GO:0004665 prephenate dehydrogenase (NADP+) activity GO:0008977 prephenate dehydrogenase activity

Sequence

>putative prephenate dehydrogenase [putative prephenate dehydrogenase]
MTLERTLIVGTGLIGTSAALALREKGVAVYLSDVDAHAVRLARALGAGQEWTGQRVDLAL
IAVPPPSVGQRLADLQQRRAARAYTDVTSVKVDPIADAERLGCDLTSYVPGHPLAGRERS
GPAAARADLFLGRPWALCPRPETGADAVRLARELVSMCGAEPYTVSAGEHDTAVALVSHA
PHVAASAVAARLRDGDDVALALAGQGLRDVTRIAAGDPLLWRMILAANALPVAGVLERIA
ADLAAAASALRSGDLDDVTDLLRRGVDGHGRIPDKHGGPARDYTVIQVVLQDRPGELARL
FNAAGLADVNIEDIRLEHSAGLPVGVVEVSVRPEDTGRLTEALRFHGWHVPPVPDGNSRI
DRTRAMVSD

>putative prephenate dehydrogenase [putative prephenate dehydrogenase]
GTGACCCTGGAGAGGACGCTCATCGTCGGCACCGGTCTGATCGGCACCTCCGCCGCGCTC
GCCCTTCGCGAGAAGGGGGTGGCGGTCTACCTGTCCGACGTCGACGCACATGCCGTACGG
CTGGCGCGAGCGCTCGGCGCGGGCCAGGAGTGGACCGGTCAGCGCGTGGACCTGGCATTG
ATCGCCGTGCCCCCGCCCAGCGTGGGGCAGCGGCTGGCCGATCTGCAGCAGCGGCGGGCC
GCGCGGGCGTACACCGATGTGACCAGCGTCAAGGTCGATCCGATCGCCGACGCGGAGCGG
CTCGGCTGCGACCTGACCTCCTATGTCCCCGGACACCCGCTCGCCGGCCGGGAGCGGTCC
GGCCCGGCCGCCGCCCGTGCCGATCTGTTCCTGGGACGTCCCTGGGCGCTCTGCCCCCGC
CCTGAGACGGGTGCGGATGCCGTGCGGCTGGCCAGGGAGCTGGTCTCGATGTGCGGGGCG
GAGCCCTACACCGTGAGTGCGGGCGAGCACGACACGGCGGTGGCGCTGGTGTCGCACGCC
CCGCACGTGGCCGCGTCCGCGGTGGCGGCGCGGCTGAGGGACGGCGACGACGTCGCGCTG
GCCCTGGCGGGGCAGGGGCTGCGCGACGTGACGCGGATCGCCGCAGGGGACCCCCTGCTG
TGGCGGATGATTCTCGCCGCGAACGCCCTGCCGGTGGCCGGGGTGCTGGAGCGGATCGCG
GCCGATCTCGCCGCGGCGGCCTCGGCGCTGCGGTCCGGCGATCTCGACGATGTGACGGAT
CTGCTGCGGCGCGGCGTGGACGGCCACGGCCGGATCCCCGACAAGCACGGCGGCCCGGCG
CGTGACTACACGGTGATCCAGGTGGTGCTGCAGGATCGGCCGGGAGAGCTGGCGAGGCTC
TTCAACGCGGCGGGGCTCGCGGACGTCAACATCGAGGACATCCGCCTGGAGCACTCGGCC
GGCCTGCCGGTCGGGGTGGTCGAGGTCTCCGTGCGCCCGGAGGACACCGGCCGGCTCACC
GAGGCACTGCGCTTCCACGGCTGGCACGTCCCGCCCGTCCCCGACGGCAACTCGAGGATC
GACCGGACGCGAGCTATGGTGTCAGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002912 ACT domain (Domain) PF01842 ACT IPR003099 Prephenate dehydrogenase (Domain) PF02153 PDH PS51176 PDH_ADH IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

A4092_00100 A4092_00120