A4092_00260 A4092_00260   A4092_00280 A4092_00280

A4092_00270 : CDS information

close this sectionLocation

Organism
StrainATCC 39727
Entry nameA40926
Contig
Start / Stop / Direction48,786 / 47,974 / - [in whole cluster]
48,786 / 47,974 / - [in contig]
Locationcomplement(47974..48786) [in whole cluster]
complement(47974..48786) [in contig]
TypeCDS
Length813 bp (270 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productdeacetylase
Product (GenBank)Dbv21 protein
Gene
Gene (GenBank)dbv21
EC number
Keyword
  • N-acyl-aminoglucuronic acid moiety
Note
Note (GenBank)
Reference
ACC
PmId
[12837387] The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. (Chem Biol. , 2003)
[17044690] Glycopeptide biosynthesis: Dbv21/Orf2 from dbv/tcp gene clusters are N-Ac-Glm teicoplanin pseudoaglycone deacetylases and Orf15 from cep gene cluster is a Glc-1-P thymidyltransferase. (J Am Chem Soc. , 2006)
[17873036] Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. (J Bacteriol. , 2007)
[18559264] Crystal structures of lipoglycopeptide antibiotic deacetylases: implications for the biosynthesis of A40926 and teicoplanin. (Chem Biol. , 2008)
[25986904] Two Master Switch Regulators Trigger A40926 Biosynthesis in Nonomuraea sp. Strain ATCC 39727. (J Bacteriol. , 2015)
comment
[PMID: 12837387](2003)
Nonomuraea species ATCC39727由来A40926生合成gene clusterの同定論文。

dbv ORF21: unknown

ORF21は知られた機能のタンパクへの有意な一致はないが、bal and cep clustersにもhomologsがある。配列解析のみ。

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[PMID: 17044690](2006) abstract
Dbv21はN-acetylglucosaminyl-teicoplanin pseudoaglyconeの脱アセチル化反応を触媒する。

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[PMID: 17873036](2007)
RT-PCRとその産物のシークエンスで、dbv21-dbv20が同時転写されることを確認。
リン酸による遺伝子発現への影響がRT-PCRで調査されている。dbv20は影響を受けない。

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[PMID: 18559264](2008)
pseudoaglycone deacetylases Orf2*(teicoplanin) と Dbv21(A40926)の結晶構造解析。

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[PMID: 25986904](2015)
dbv clusterにあるregulatory genes dbv3, dbv4, dbv6についての調査。

dbv3の不活化株と過剰発現株からRNAを抽出してqRT-PCR解析した結果と既に確立されているオペロン構造から、Dbv3は6つのオペロン(dbv2-dbv1, dbv14-dbv8, dbv17-dbv15, dbv21-dbv20, dbv24-dbv28, dbv30-dbv35)と4つのmonocistronic transcription units dbv4, dbv29, dbv36, dbv37のpositive regulatorとして働くことが示された。

close this sectionSequence

selected fasta
>deacetylase [Dbv21 protein]
MLQDADRTRILAISPHLDDAVLSVGASLAQAEQDGGKVTVFTVFAGSAAPPYSPAAERFH
ARWGLSPTEDAPLRRRNEDIAALDQLGAGHRHGRFLDAIYRRSPDGQWLLHHNEGSMVRQ
QSPANNHDLVAAIREDIESMIAECDPTLVLTCVAIGKHPDHKATRDATLLAARERGIPLR
LWQDLPYAAYSQDLAELPDGLRLGSPELSFVDEEARTRKFQAMKHYATQLSVLDGPNKNL
FAKLDEHARNAAPDGGYNETTWPVIRYAAE
selected fasta
>deacetylase [Dbv21 protein]
ATGTTGCAGGACGCCGACCGAACCCGGATATTAGCAATCTCCCCTCATTTGGATGATGCC
GTCCTCTCCGTCGGAGCCAGCCTCGCCCAAGCGGAGCAGGACGGTGGAAAAGTAACCGTC
TTCACGGTGTTCGCCGGCAGCGCGGCGCCCCCTTATTCCCCGGCGGCGGAGCGATTCCAC
GCACGCTGGGGTCTGTCGCCGACCGAGGACGCGCCGCTGCGCCGACGAAATGAGGACATC
GCCGCCCTCGATCAGCTCGGTGCGGGCCATCGGCATGGCCGTTTTCTCGATGCCATCTAT
CGAAGGTCACCGGATGGCCAGTGGCTGCTGCACCACAACGAAGGCTCGATGGTCCGCCAG
CAGTCGCCGGCGAACAACCACGACCTGGTCGCCGCGATCAGGGAGGACATCGAGTCCATG
ATCGCGGAGTGCGATCCGACGCTCGTTCTCACCTGTGTGGCCATCGGGAAACACCCCGAC
CACAAGGCCACGCGGGATGCCACGCTGCTCGCCGCACGCGAGCGAGGTATTCCGCTGCGA
CTCTGGCAGGATCTCCCGTACGCGGCGTATTCCCAGGACCTGGCTGAATTGCCTGACGGC
CTCCGGCTCGGCTCTCCGGAGCTGAGTTTCGTCGATGAGGAGGCGCGGACGCGGAAATTC
CAGGCGATGAAGCACTACGCGACGCAGTTGTCGGTGCTCGACGGGCCGAACAAGAACCTG
TTCGCCAAACTCGATGAGCATGCCAGGAACGCCGCACCGGACGGCGGATATAACGAGACC
ACCTGGCCGGTCATTCGCTACGCAGCCGAGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003737 N-acetylglucosaminyl phosphatidylinositol deacetylase (Family)
[11-171] 2.90000000000001e-20 PF02585
PF02585   PIG-L
IPR024078 Putative deacetylase LmbE-like domain (Domain)
[6-247] 2.9000035467798e-41 SSF102588
SSF102588   SSF102588
[7-241] 1.1e-28 G3DSA:3.40.50.10320
G3DSA:3.40.50.10320   G3DSA:3.40.50.10320
SignalP No significant hit
TMHMM No significant hit
A4092_00260 A4092_00260   A4092_00280 A4092_00280
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