A4092_00300 A4092_00300   A4092_00320 A4092_00320

A4092_00310 : CDS information

close this sectionLocation

Organism
StrainATCC 39727
Entry nameA40926
Contig
Start / Stop / Direction54,777 / 61,070 / + [in whole cluster]
54,777 / 61,070 / + [in contig]
Location54777..61070 [in whole cluster]
54777..61070 [in contig]
TypeCDS
Length6,294 bp (2,097 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)putative non-ribosomal peptide synthetase, modules 1-2
Gene
Gene (GenBank)dbv25
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12837387] The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. (Chem Biol. , 2003)
[17873036] Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. (J Bacteriol. , 2007)
[25986904] Two Master Switch Regulators Trigger A40926 Biosynthesis in Nonomuraea sp. Strain ATCC 39727. (J Bacteriol. , 2015)
comment
[PMID: 12837387](2003)
Nonomuraea sp. ATCC39727由来A40926生合成gene clusterの同定論文。
A40926は半合成グリコペプチドdalbavancin (BI397 or MDL 62,397)の前駆体。

dbv ORF25: NRPS, modules 1-2

配列解析のみ。dbv ORF25のドメイン構成はA-T-C-A-T-Eで、module 1 と 2をコードする。

---
[PMID: 17873036](2007)
RT-PCRとその産物のシークエンスで、dbv24-dbv28が同時転写されることを確認。
リン酸による遺伝子発現への影響がRT-PCRで調査されている。dbv25 と dbv28は影響を受けない。

---
[PMID: 25986904](2015)
dbv clusterにあるregulatory genes dbv3, dbv4, dbv6についての調査。

dbv3の不活化株と過剰発現株からRNAを抽出してqRT-PCR解析した結果と既に確立されているオペロン構造から、Dbv3は6つのオペロン(dbv2-dbv1, dbv14-dbv8, dbv17-dbv15, dbv21-dbv20, dbv24-dbv28, dbv30-dbv35)と4つのmonocistronic transcription units dbv4, dbv29, dbv36, dbv37のpositive regulatorとして働くことが示された。

close this sectionPKS/NRPS Module

A1 L-4-hydroxyphenylglycine(Hpg)
2 tyrosine
A37..427
PCP503..571
C602..897
A1064..1454
PCP1530..1599
E1635..2082

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [putative non-ribosomal peptide synthetase, modules 1-2]
MSAGTRATPTTVLDLFARQVGRAPDAVALVDGDRVLTYRRLDELAGALSGRLIGRGVGRG
DRVAVMMDRSADLVVTLLAVWQAGAAYVPVDAALPARRVAFMVADSGACLMVCSEATRDA
VPQGVESIALTGEGGCGTSAVTVDPGDLAYVMYTSGSTGTPKGVAVPHRSVAELTGNPGW
GVEPGEAVLMHAPYTFDASLFEIWVPLVSGARVVIAAPGAVDARRLREAVAAGVTRVHLT
AGSFRAVAEESPESFAHFREVLTGGDVVPAYAVQKVRAACPHVRIRHLYGPTETTLCATW
QLLEPGDVVGPVLPIGRPLPGRRAWVLDASLRPVEPGVVGDLYLSGAGLADGYLDRAGLT
AERFVADPSAAGRRMYRTGDLAQWTADGELLFAGRADDQVKVRGFRIEPGEVEAALTAQP
HVREAVVVAIDGRLIGYVVADGDVDPVLMRRRLAASLPEYMIPAALVTLDALPLTGSGKV
DRRALPEPDFASAAPRREPGTEPERVLCDLFAELLQPEGRGVGVDDGFVELGGDSIVAIR
LAARASRVGLLVTPAQIFKEKTPARLAAVAGAVPAGRPADGPLITLTAEEEAELATAVPG
AEEVWPLAPLQEGLYFQATLDDEGHDIYQAQWILELAGPLDAARLRASWEAVFARHPELR
VSFHRRASGTMLQVVAGHVVLPWREVDLADAGDIDAAVAALISEEQEQRFDLAKAPLFRL
VLVRHGEDRHRLLVVHHHILTDGWSVAVILNEVAEAYTNGGRLPDRTGAASYRDYLAWLD
RQDKDAARAAWQAELSGLEGPAPIAKAATTTGAGTGYEYRIAFLTPDLHTRLTELARDHG
LTLNTLAQGAWAMVLARLARRTDVVFGTTVACRPAELPEVESVPGLMMNTVPVRVPLQGA
QSVVDLLTGLQERQAALLPHQHLGLTEIQRAAGPGATFDTLLVFENYPRDFAGQFTYLGT
IEGTHYPLTLGIIPGDHFRIQLVYRRGQVGESVAESILGWFTGALMTMAADPHGPVGRIG
VGEARAGGSDRAMAAGEPLPVLLRRVVKDRPDEVAVVDGDGELSFGELWERATALAAELR
AHGIGPESRVAVMVGRSAWWAVGVLGVCLAGGAFMPVDPAYPAERVRWILADSDPRLVLC
AGTTREAVPEEFADRLVVVDELDLAGSDDAGLPRVSPDDAAYVIYTSGSTGTPKGVVVSH
AGLGNLAMAQIDRFAVSPSSRVLQFAALGFDAMVSEMLMALLSGARLVMAPEPALPPRVS
LAEALRRWEVTHVTVPPSVLATADALPAGLETVVVAGEACPPGLAERWSAGRRLVNAYGP
TEATVCAAMSRPLTGSREVVPIGTPIAGGRCYVLDAFLRPLPPGITGELYVAGIGLARGY
LGRASLTAERFVADPFVAGERMYRTGDLAYWTGEGELVFAGRDDDQVKIRGYRVEPGEVE
AVLAGQPGVDQAVVVAREGRLLGYVVSGGGVDPVRLREGVARVLPEYMVPAAVVVLGAVP
VTANGKVDREALPDPGFGGRVSGREPRTEVERALCGLFAEVLGLPGVTAVGPDDSFFELG
GDSITSMQLASRARREGMLFGAREVFERKTPAGLAAIVDVGGELAAGPADGVGEIAWTPI
MRALGDGIVGSRFAQWVVLGAPPDLRADVVAAGLAAVVDTHDVLRLRVVDDRAGRRLAVG
ERGSVDTAGLVTRLECGGRPPDEVVERAVREAVGRLDPVAGVMAQAVWVDAGPARTGRLV
VVVHHLAVDGMSWRILVPDLRLACEAVAEGRDPVLEPVWGSFRRWAALLEESALSRERVG
ELHTWRTIVDQEDRPVGRRRLSAGDAAGGVRSRSWVMSGDEASLLVGKVPVAFHCGVHEV
LLAGLAGAVARWHGDDGVLVDVEGHGRHPAEGMDLSRTVGWFTSMHPVRLDVAGIELAAV
PAGGRAAGQLLKAVKEQSRAAPGDGLGYGLLRHLNPETGPVLAALPSPQIGFNYMGRFVT
VDQGGARPWQPVGGIGGSLDPGMGLPHALEVNAIVHDRLAGPELVLTVDWRDDLLEETDI
ERLCQVWLDMLSGLSRQAEDPSAGGHTASDFALLDLDQDEIEGFEAIAAELSGGQTS
selected fasta
>non-ribosomal peptide synthetase [putative non-ribosomal peptide synthetase, modules 1-2]
ATGAGCGCCGGAACGCGGGCCACACCGACGACGGTGCTGGACCTCTTCGCCCGCCAGGTG
GGCCGGGCACCCGATGCGGTGGCTCTGGTCGACGGGGACCGGGTCCTGACCTACCGGCGG
CTGGACGAGCTCGCCGGAGCGCTGTCCGGGCGCCTGATCGGCCGGGGTGTCGGCCGGGGT
GATCGCGTCGCGGTGATGATGGACCGCTCGGCGGACCTGGTGGTGACGCTGCTCGCCGTG
TGGCAGGCGGGGGCGGCCTACGTGCCGGTGGACGCCGCCCTTCCCGCCCGGCGGGTGGCG
TTCATGGTGGCGGACTCCGGAGCCTGCCTGATGGTGTGCTCGGAGGCGACGCGCGATGCG
GTACCGCAAGGGGTCGAGTCGATCGCGTTGACCGGCGAGGGCGGATGCGGCACGTCGGCG
GTCACGGTGGACCCGGGGGATCTGGCGTACGTGATGTACACGTCCGGCTCGACGGGCACC
CCGAAGGGGGTGGCCGTCCCGCATCGGAGCGTCGCGGAGCTGACGGGAAACCCCGGCTGG
GGGGTGGAGCCCGGCGAGGCGGTGCTCATGCACGCGCCCTACACCTTCGACGCCTCCCTG
TTCGAGATCTGGGTGCCGCTCGTGTCGGGCGCCCGGGTGGTGATCGCCGCACCGGGTGCG
GTGGACGCCCGGCGCCTGCGCGAGGCGGTCGCCGCCGGGGTGACGAGGGTGCACCTGACC
GCGGGCAGCTTCCGCGCGGTGGCGGAGGAGTCGCCGGAGTCGTTCGCGCACTTCCGTGAG
GTGCTGACCGGTGGTGACGTGGTGCCCGCGTACGCAGTGCAGAAGGTGCGGGCGGCCTGC
CCTCACGTGCGGATCCGGCATCTGTACGGCCCGACGGAGACGACCCTGTGCGCGACGTGG
CAGCTGCTGGAGCCGGGTGACGTCGTGGGGCCCGTCCTGCCGATCGGCCGCCCGCTGCCG
GGCCGCCGGGCCTGGGTGCTCGACGCGTCATTGCGGCCGGTGGAGCCCGGGGTGGTCGGT
GACCTGTACCTTTCCGGCGCCGGTCTGGCGGACGGCTACCTGGACCGGGCGGGGCTGACG
GCGGAACGGTTCGTGGCGGATCCGTCCGCGGCGGGGAGGCGGATGTATCGGACGGGGGAT
CTGGCTCAGTGGACCGCGGACGGTGAGCTGCTGTTCGCGGGCCGGGCCGACGACCAGGTG
AAGGTCCGCGGATTCCGGATCGAGCCGGGCGAGGTCGAGGCCGCGCTGACCGCTCAGCCG
CACGTCCGCGAGGCCGTGGTGGTGGCGATCGACGGGCGCCTGATCGGTTACGTGGTGGCG
GACGGGGACGTGGATCCCGTACTGATGCGCCGGCGGCTGGCGGCGTCGCTGCCGGAGTAC
ATGATCCCGGCCGCTCTGGTCACGCTGGACGCGTTGCCGCTGACCGGCAGCGGCAAGGTG
GACAGGAGGGCGCTGCCCGAGCCCGATTTCGCGTCGGCCGCGCCGCGCCGCGAACCCGGC
ACCGAGCCGGAGCGCGTCCTGTGCGACCTGTTCGCGGAGCTTCTGCAACCGGAGGGAAGG
GGGGTAGGGGTCGATGACGGTTTCGTCGAGCTGGGCGGGGACTCGATCGTCGCGATCCGG
CTGGCAGCACGTGCGTCCAGGGTGGGGCTGCTGGTGACGCCCGCCCAGATCTTCAAGGAG
AAGACTCCGGCACGGCTGGCAGCCGTCGCGGGTGCCGTACCGGCCGGCAGACCCGCCGAC
GGCCCGCTGATCACCCTCACGGCGGAGGAGGAGGCGGAGCTGGCGACCGCCGTCCCGGGG
GCCGAGGAGGTCTGGCCACTCGCACCGCTCCAGGAAGGGCTCTACTTCCAGGCCACCCTC
GACGACGAGGGTCACGACATCTACCAGGCGCAATGGATCCTGGAGCTGGCGGGGCCGCTG
GACGCCGCCCGGCTGCGGGCCTCGTGGGAAGCGGTCTTCGCCCGGCACCCCGAGCTTCGC
GTGAGCTTCCACCGGCGCGCGTCAGGCACGATGCTGCAGGTCGTCGCCGGGCACGTCGTC
CTGCCGTGGCGAGAGGTGGATCTGGCGGATGCGGGCGATATCGACGCGGCCGTGGCGGCC
CTGATCAGTGAGGAACAGGAGCAGCGGTTCGACCTCGCCAAGGCACCGCTGTTCCGGCTG
GTGCTGGTCCGTCACGGCGAGGACCGGCACCGCCTGCTGGTCGTCCATCACCACATCCTG
ACCGATGGCTGGTCGGTGGCGGTCATCCTCAACGAGGTGGCTGAGGCGTACACGAACGGC
GGCCGTCTCCCGGACCGCACGGGCGCGGCCTCCTACCGGGACTACCTGGCCTGGCTGGAC
CGGCAGGACAAGGACGCCGCACGTGCCGCCTGGCAGGCGGAGCTGTCCGGCCTCGAAGGG
CCCGCGCCGATCGCGAAGGCCGCCACCACGACCGGCGCCGGGACGGGCTACGAATATCGC
ATCGCCTTCCTGACCCCTGACCTCCACACGCGGCTGACGGAGCTGGCCCGCGACCACGGG
CTGACGCTGAACACCCTGGCACAGGGCGCATGGGCGATGGTGCTGGCGCGGCTCGCGCGG
CGCACTGACGTGGTCTTCGGCACCACGGTCGCCTGCCGTCCCGCCGAGCTCCCCGAGGTG
GAGTCGGTGCCGGGTCTCATGATGAACACGGTTCCGGTCCGGGTGCCGCTGCAGGGCGCG
CAATCGGTCGTGGACCTGCTCACCGGCCTGCAGGAACGGCAGGCGGCCTTGCTGCCGCAC
CAGCATCTGGGGCTGACGGAGATCCAGCGGGCGGCAGGACCTGGCGCGACGTTCGACACG
CTGCTGGTCTTCGAGAACTACCCGCGGGACTTCGCCGGCCAGTTCACCTACCTGGGCACG
ATCGAGGGGACCCACTACCCGCTGACCCTCGGCATCATCCCGGGGGATCACTTCAGGATC
CAGCTCGTCTACCGGCGCGGGCAGGTCGGGGAGAGCGTCGCCGAGTCGATCCTGGGATGG
TTCACCGGCGCTCTCATGACGATGGCCGCTGATCCGCACGGGCCGGTGGGCCGGATCGGT
GTGGGTGAGGCCCGGGCCGGCGGCTCGGACCGGGCGATGGCGGCGGGGGAGCCGCTGCCG
GTGCTGCTACGGCGGGTGGTGAAGGACCGGCCGGACGAGGTGGCGGTGGTGGACGGCGAC
GGTGAGCTGTCGTTCGGGGAATTGTGGGAACGGGCGACGGCGCTGGCGGCCGAGCTGAGG
GCTCACGGGATCGGGCCGGAGAGCCGGGTGGCCGTGATGGTGGGCAGGTCGGCGTGGTGG
GCGGTCGGGGTGCTGGGCGTCTGCTTGGCGGGCGGCGCGTTCATGCCGGTGGATCCGGCG
TATCCGGCTGAGCGCGTCAGGTGGATCCTGGCCGACTCCGACCCACGGCTGGTGCTGTGC
GCGGGGACGACGCGGGAGGCGGTGCCGGAGGAGTTCGCAGACCGGCTGGTGGTGGTCGAC
GAGCTGGACCTCGCGGGGAGCGACGATGCGGGCTTGCCACGGGTGAGCCCGGATGACGCG
GCTTATGTGATCTATACGTCGGGATCGACGGGGACTCCCAAGGGGGTCGTCGTCTCGCAC
GCGGGCCTCGGGAATCTGGCGATGGCGCAGATCGACCGGTTCGCCGTGTCGCCGTCGTCG
CGAGTCCTGCAGTTCGCGGCGCTGGGCTTCGACGCGATGGTGTCGGAGATGTTGATGGCG
CTGTTGTCGGGGGCGAGGCTGGTGATGGCGCCGGAGCCGGCCCTGCCACCGCGGGTGTCG
CTGGCCGAGGCGTTGCGGCGGTGGGAGGTCACGCACGTCACGGTTCCGCCGTCGGTGCTG
GCCACCGCCGATGCGCTGCCGGCCGGGCTGGAGACGGTGGTGGTGGCGGGGGAGGCCTGC
CCGCCGGGCCTGGCCGAACGCTGGTCGGCGGGACGGCGGCTGGTCAACGCGTACGGGCCG
ACCGAGGCCACGGTCTGCGCGGCGATGAGCAGGCCGTTGACGGGCAGCCGGGAGGTGGTC
CCGATCGGGACACCCATCGCCGGCGGCCGTTGCTACGTGCTGGACGCGTTCCTGCGGCCG
TTGCCGCCGGGGATCACCGGTGAGCTGTACGTGGCCGGGATCGGGTTGGCGCGCGGCTAT
CTGGGTCGTGCGTCGTTGACGGCTGAGCGGTTCGTGGCGGATCCGTTCGTGGCTGGTGAG
CGGATGTATCGGACGGGGGATCTGGCGTATTGGACGGGTGAGGGCGAGCTGGTGTTCGCC
GGGCGGGATGACGACCAGGTGAAGATCCGTGGGTATCGGGTGGAGCCGGGTGAGGTGGAG
GCGGTGCTGGCGGGGCAGCCGGGGGTGGATCAGGCGGTGGTGGTGGCGCGTGAGGGGCGG
TTGCTGGGTTATGTCGTCTCCGGTGGTGGGGTGGATCCGGTGCGGTTGCGTGAGGGGGTC
GCGCGGGTGTTGCCGGAGTACATGGTGCCGGCGGCGGTGGTGGTGCTGGGTGCGGTGCCG
GTGACGGCGAACGGGAAGGTGGATCGGGAGGCGTTGCCGGATCCGGGCTTCGGCGGGCGG
GTTTCGGGCCGGGAGCCGCGTACGGAGGTCGAGCGGGCGTTGTGCGGGCTCTTCGCCGAG
GTGCTCGGGCTGCCGGGGGTGACGGCGGTGGGGCCGGACGACAGCTTCTTCGAGCTGGGC
GGGGACTCGATCACTTCGATGCAGCTGGCGTCGCGGGCTCGCCGCGAGGGGATGCTCTTC
GGCGCGCGGGAGGTGTTCGAGCGCAAGACGCCTGCGGGGCTCGCGGCGATCGTCGATGTG
GGCGGCGAGCTTGCGGCAGGTCCGGCCGACGGCGTGGGGGAGATCGCGTGGACGCCGATC
ATGCGGGCGCTCGGGGACGGGATCGTGGGGTCGCGGTTCGCCCAGTGGGTGGTGCTGGGT
GCGCCGCCGGACCTACGGGCGGACGTGGTGGCCGCGGGATTGGCGGCGGTGGTGGACACG
CACGACGTGTTGCGGCTGCGGGTCGTCGATGACCGGGCGGGCCGCCGGCTGGCAGTGGGC
GAGCGCGGGTCGGTGGACACGGCCGGGCTGGTCACGCGGCTCGAGTGCGGCGGCCGTCCG
CCGGACGAGGTCGTGGAGCGCGCGGTGCGGGAGGCCGTGGGGCGGTTGGACCCGGTGGCG
GGTGTGATGGCGCAGGCGGTCTGGGTGGATGCGGGGCCGGCGCGGACGGGGCGGTTGGTC
GTCGTGGTGCATCATCTGGCGGTCGACGGGATGTCGTGGCGGATCCTGGTGCCCGACCTG
CGGCTGGCGTGTGAGGCGGTGGCCGAGGGGCGGGATCCGGTGCTGGAGCCGGTGTGGGGG
TCGTTCCGGCGCTGGGCGGCTCTGCTGGAGGAGTCGGCGCTCTCGCGGGAGCGGGTCGGG
GAGCTGCACACGTGGCGGACGATCGTCGATCAGGAGGATCGGCCGGTCGGCCGGCGGCGG
CTGAGCGCAGGGGATGCGGCCGGGGGCGTGCGTTCACGGTCGTGGGTGATGTCGGGGGAT
GAGGCGTCGCTCCTGGTGGGGAAGGTTCCGGTGGCGTTCCACTGCGGGGTCCACGAGGTC
CTGCTGGCGGGCCTGGCGGGAGCGGTGGCGCGCTGGCACGGTGACGACGGGGTCCTGGTG
GATGTGGAAGGCCACGGGCGTCATCCGGCCGAGGGGATGGATCTGTCCAGGACGGTGGGC
TGGTTCACCAGCATGCATCCGGTGCGCCTGGATGTGGCGGGGATCGAGCTGGCGGCGGTG
CCGGCCGGTGGCCGTGCGGCCGGGCAGTTGCTGAAGGCGGTCAAGGAGCAGTCGCGGGCG
GCGCCCGGCGACGGGCTCGGTTACGGGTTGCTGCGCCATCTCAATCCCGAGACGGGCCCC
GTTCTGGCGGCCCTGCCGTCACCGCAGATCGGGTTCAACTACATGGGCCGGTTCGTCACC
GTCGACCAGGGCGGTGCGCGGCCGTGGCAGCCGGTCGGGGGGATCGGCGGTTCGCTGGAC
CCCGGCATGGGCCTGCCGCATGCGCTGGAGGTCAATGCGATCGTCCACGACAGGCTGGCG
GGCCCGGAGCTGGTGCTCACGGTGGACTGGCGGGACGACCTGCTGGAGGAGACCGACATC
GAACGACTGTGCCAGGTGTGGCTGGACATGTTGTCCGGATTGTCCCGCCAAGCGGAGGAT
CCTTCCGCAGGCGGACACACCGCGTCCGACTTCGCCCTACTCGACCTCGACCAGGACGAG
ATCGAGGGCTTCGAAGCCATAGCAGCGGAACTCTCTGGAGGCCAGACATCGTGA
[1] A37..427
[1] L-4-hydroxyphenylglycine(Hpg)197..297
[1] PCP503..571
[2] C602..897
[2] A1064..1454
[2] tyrosine1231..1326
[2] PCP1530..1599
[2] E1635..2082
[1] A109..1281
[1] L-4-hydroxyphenylglycine(Hpg)589..891
[1] PCP1507..1713
[2] C1804..2691
[2] A3190..4362
[2] tyrosine3691..3978
[2] PCP4588..4797
[2] E4903..6246

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[37-427] 1.20000000000001e-103 PF00501 [1064-1454] 3.39999999999996e-112 PF00501
PF00501   AMP-binding
IPR001242 Condensation domain (Domain)
[602-897] 3.99999999999998e-62 PF00668 [1635-1912] 3.2e-26 PF00668
PF00668   Condensation
IPR006162 Phosphopantetheine attachment site (PTM)
[530-545] PS00012 [1558-1573] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[503-571] PS50075 [1530-1599] PS50075
PS50075   ACP_DOMAIN
[493-570] 2.1e-43 G3DSA:1.10.1200.10 [1522-1599] 2.1e-43 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[495-588] 3.00000067992871e-13 SSF47336 [1523-1598] 1.10000150671642e-17 SSF47336
SSF47336   ACP_like
[506-568] 1.8e-08 PF00550 [1533-1596] 3.8e-11 PF00550
PF00550   PP-binding
IPR010060 Non-ribosomal peptide synthase (Domain)
[1927-2082] 7.4e-36 TIGR01720
TIGR01720   NRPS-para261
IPR010071 Amino acid adenylation domain (Domain)
[37-427] 1.39999999999997e-123 TIGR01733 [1064-1454] 3.10000000000005e-128 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020845 AMP-binding, conserved site (Conserved_site)
[151-162] PS00455 [1183-1194] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
A4092_00300 A4092_00300   A4092_00320 A4092_00320
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