A4092_00310 A4092_00310   A4092_00330 A4092_00330

A4092_00320 : CDS information

close this sectionLocation

Organism
StrainATCC 39727
Entry nameA40926
Contig
Start / Stop / Direction61,067 / 64,258 / + [in whole cluster]
61,067 / 64,258 / + [in contig]
Location61067..64258 [in whole cluster]
61067..64258 [in contig]
TypeCDS
Length3,192 bp (1,063 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)putative non-ribosomal peptide synthetase, module 3
Gene
Gene (GenBank)dbv26
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12837387] The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. (Chem Biol. , 2003)
[17873036] Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. (J Bacteriol. , 2007)
[25986904] Two Master Switch Regulators Trigger A40926 Biosynthesis in Nonomuraea sp. Strain ATCC 39727. (J Bacteriol. , 2015)
comment
[PMID: 12837387](2003)
Nonomuraea sp. ATCC39727由来A40926生合成gene clusterの同定論文。
A40926は半合成グリコペプチドdalbavancin (BI397 or MDL 62,397)の前駆体。

dbv ORF26: NRPS, module 3

配列解析のみ。dbv ORF26のドメイン構成はC-A-Tで、module 3をコードする。

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[PMID: 17873036](2007)
RT-PCRとその産物のシークエンスで、dbv24-dbv28が同時転写されることを確認。
リン酸による遺伝子発現への影響がRT-PCRで調査されている。dbv25 と dbv28は影響を受けない。

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[PMID: 25986904](2015)
dbv clusterにあるregulatory genes dbv3, dbv4, dbv6についての調査。

dbv3の不活化株と過剰発現株からRNAを抽出してqRT-PCR解析した結果と既に確立されているオペロン構造から、Dbv3は6つのオペロン(dbv2-dbv1, dbv14-dbv8, dbv17-dbv15, dbv21-dbv20, dbv24-dbv28, dbv30-dbv35)と4つのmonocistronic transcription units dbv4, dbv29, dbv36, dbv37のpositive regulatorとして働くことが示された。

close this sectionPKS/NRPS Module

A3 L-3,5-dihydroxyphenylglycine(Dpg)
C13..308
A487..877
PCP953..1022

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [putative non-ribosomal peptide synthetase, module 3]
MNTPSTPAGSALEEVWPLSPMQEGILYHAALDEAPDLYLIQQSQIIEGPLDTERFRLAWE
SLLNRHAALRACFHRRKSGESVQLIPRKVPLPWSERDLSGLSEEDALAEASVIAEKERAT
RFDPAKPPLLRQVLIRFGPDKHCLVTTSHHLVMDGWSRAILESELLELYAAGGAEPGLRP
AGSYRDYLAWLERQDKEAARAAWRAELAGADRSTLGIPEASRKTQGQRVREVLGYAPDFT
SALVDFARRHGLTLNTLVQGAWALVLARLTRRRDVVFGAVVSGRPAEVPGVEQAVGLFIN
TVPVRVRLDGGQPVIQLLTELQERQSTLISHQHLGLQEIQKLSGVSFDTVVSFENYVDPG
AGPGSDRELRLRLKEFHQSAPYALLLGIMPGESLQTDVEYRPELLDARVAKEALHGLARV
LERMIAEPETAVGRLDVVGDAGRELVVERWNETGDAIGAPSAVDLFRRQVARAPAATAVT
AGDLAWSYAELDERSGRLARALTERGVRRGDRVGVVLGRSAEVLAAWLGVWKAGAAFVPV
DPDYPADRVAFMLADSAVAMVVCQEATSGVVPPGYQQLLVNDADDGEAALVPIGADDLAY
VMYTSGSTGTPKGVAIPHGGVAALAGDPGWGVGPGDAVLMHAPHTFDASLYDVWVPLVSG
ARVMITEPGVVDAERLAGHVADGLTAVNFTAGHFRALAQESPESFSGLREVAAGGDVVPL
DVVERVRRACPRLRVWHTYGPTETTLCATWKAIEPGDEVGPVLPIGRALPGRRLYVLDAF
LRPLPPGIAGDLYLAGAGVAHGYLGRASLTAERFVADPFVAGERMYRTGDLAYWTGEGEL
VFAGRDDDQVKIRGYRVEPGEVEAVLAGQPGVDQAVVVAREGRLLGYVVSGGGVDPVRLR
EGVARVLPEYMVPAAVVVLGAVPVTANGKVDREALPDPGFGGRVSGREPRTEVERALCGL
FAEVLGLPGVTAVGPDDSFFELGGDSIHSVKLAARATRAGMPFTVVEVFEHKTPAGLATI
VDVGGEPAAGPADPPSDSDLLGLAQDEIAEFEAEFDDERHSLR
selected fasta
>non-ribosomal peptide synthetase [putative non-ribosomal peptide synthetase, module 3]
GTGAACACGCCGAGCACACCCGCCGGATCGGCGCTTGAGGAAGTCTGGCCGCTGTCACCG
ATGCAGGAGGGGATCCTCTATCACGCCGCACTCGATGAGGCCCCTGACCTCTACCTCATC
CAGCAGTCGCAGATCATCGAAGGACCCTTGGACACCGAGCGGTTCCGCCTGGCTTGGGAG
AGCCTCCTCAACCGGCATGCGGCGCTTCGCGCGTGCTTCCACCGGCGGAAGTCCGGTGAG
TCGGTCCAGCTCATCCCCCGTAAGGTGCCGCTCCCGTGGTCCGAGCGCGACCTGTCCGGC
CTGTCCGAGGAGGACGCGCTGGCCGAGGCGAGCGTGATCGCGGAGAAGGAGCGCGCCACG
AGATTCGACCCGGCCAAGCCTCCGCTGCTGCGGCAGGTGCTGATCCGGTTCGGTCCGGAC
AAGCACTGTCTGGTGACGACGAGCCATCACCTGGTCATGGACGGGTGGTCGCGGGCGATC
CTCGAGTCGGAGCTCCTCGAGCTCTACGCCGCGGGTGGCGCCGAGCCGGGGCTGCGGCCC
GCCGGCTCCTACCGGGACTATCTGGCCTGGCTGGAGCGGCAGGACAAGGAGGCCGCCCGC
GCGGCATGGCGTGCGGAGCTGGCGGGCGCCGACCGTTCGACACTCGGCATCCCCGAAGCG
TCCAGGAAGACCCAGGGGCAGCGGGTGCGGGAGGTGCTCGGCTACGCGCCGGACTTCACC
TCCGCTCTGGTGGACTTCGCCCGCCGCCATGGGCTGACGCTGAACACGCTGGTGCAGGGG
GCGTGGGCGTTGGTGCTGGCCCGGCTCACGCGCCGTCGTGACGTGGTGTTCGGCGCGGTG
GTCTCGGGACGTCCGGCGGAGGTGCCCGGCGTGGAGCAGGCCGTCGGGCTGTTCATCAAC
ACCGTGCCGGTGCGCGTCCGGTTGGACGGCGGGCAGCCGGTCATCCAGCTGCTGACGGAG
CTGCAGGAGCGGCAGTCCACGCTCATCTCGCATCAGCATCTCGGGCTGCAGGAGATCCAG
AAGCTCTCCGGGGTGAGCTTCGACACCGTCGTGTCGTTCGAGAACTACGTCGATCCGGGG
GCGGGTCCGGGCTCCGATCGCGAGCTGCGCCTGAGACTGAAGGAGTTTCACCAGTCGGCG
CCGTACGCGCTCCTCCTCGGCATCATGCCAGGTGAGAGCCTCCAGACCGACGTGGAGTAC
CGGCCCGAGCTGCTCGACGCCCGCGTCGCCAAGGAGGCCCTCCACGGGCTCGCCCGCGTC
CTCGAGCGGATGATCGCCGAGCCGGAGACCGCAGTGGGCCGCCTGGACGTGGTCGGTGAC
GCGGGGCGCGAGCTGGTGGTCGAGCGGTGGAACGAGACGGGCGACGCGATCGGTGCGCCG
TCCGCGGTGGACCTGTTCCGGCGCCAGGTTGCACGGGCACCCGCCGCGACGGCGGTGACG
GCCGGGGACCTGGCCTGGTCGTACGCGGAGCTCGACGAGCGGTCCGGCCGGCTGGCGCGG
GCACTGACGGAACGCGGCGTGCGACGCGGCGACCGGGTGGGCGTGGTGCTGGGGCGGTCG
GCAGAGGTGCTGGCAGCCTGGCTCGGAGTGTGGAAGGCAGGCGCGGCGTTCGTGCCGGTC
GACCCGGACTACCCGGCGGACCGGGTGGCGTTCATGCTGGCCGACTCCGCCGTCGCGATG
GTGGTGTGCCAGGAGGCGACCTCGGGTGTGGTGCCCCCGGGCTACCAGCAGCTCCTGGTG
AACGACGCCGACGACGGCGAGGCCGCCCTGGTCCCGATCGGGGCGGACGATCTCGCCTAC
GTGATGTACACCTCCGGATCGACCGGGACCCCGAAGGGCGTGGCGATCCCGCACGGCGGC
GTGGCGGCGCTGGCGGGAGATCCGGGATGGGGCGTCGGACCCGGCGACGCGGTGCTGATG
CACGCCCCGCACACCTTCGACGCGTCGTTGTACGACGTGTGGGTGCCGCTCGTCTCCGGC
GCGCGGGTCATGATCACCGAGCCGGGGGTCGTCGACGCGGAGCGGCTCGCCGGGCATGTG
GCCGACGGCCTCACCGCGGTCAACTTCACCGCGGGGCACTTCCGCGCGCTGGCGCAGGAG
TCGCCGGAGTCGTTCTCCGGGCTGCGCGAGGTGGCGGCGGGTGGCGACGTGGTGCCGCTC
GATGTGGTGGAGCGGGTACGGCGGGCGTGCCCGCGGCTCCGGGTCTGGCACACCTACGGC
CCGACCGAGACCACGCTGTGCGCGACGTGGAAGGCGATCGAGCCCGGTGACGAGGTGGGG
CCGGTGCTGCCCATCGGCCGGGCACTGCCGGGCCGGCGGCTGTACGTGCTGGACGCGTTC
CTGCGGCCGTTGCCGCCGGGCATCGCGGGTGATCTCTACCTCGCAGGCGCCGGAGTGGCC
CACGGCTATCTGGGTCGTGCGTCGTTGACGGCTGAGCGGTTCGTGGCGGATCCGTTCGTG
GCTGGTGAGCGGATGTATCGGACGGGGGATCTGGCGTATTGGACGGGTGAGGGCGAGCTG
GTGTTCGCCGGGCGGGATGACGACCAGGTGAAGATCCGTGGGTATCGGGTGGAGCCGGGT
GAGGTGGAGGCGGTGCTGGCGGGGCAGCCGGGGGTGGATCAGGCGGTGGTGGTGGCGCGT
GAGGGGCGGTTGCTGGGTTATGTCGTCTCCGGTGGTGGGGTGGATCCGGTGCGGTTGCGT
GAGGGGGTCGCGCGGGTGTTGCCGGAGTACATGGTGCCGGCGGCGGTGGTGGTGCTGGGT
GCGGTGCCGGTGACGGCGAACGGGAAGGTGGATCGGGAGGCGTTGCCGGATCCGGGCTTC
GGCGGGCGGGTTTCGGGCCGGGAGCCGCGTACGGAGGTCGAGCGGGCGTTGTGCGGGCTC
TTCGCCGAGGTGCTCGGGCTGCCGGGGGTGACGGCGGTGGGGCCGGACGACAGCTTCTTC
GAGCTGGGCGGGGACTCCATCCATTCGGTGAAGCTGGCAGCGCGGGCCACGCGTGCCGGC
ATGCCCTTCACCGTGGTCGAGGTGTTCGAGCACAAGACGCCTGCGGGGCTCGCGACGATC
GTCGACGTGGGCGGCGAGCCCGCGGCAGGTCCGGCTGATCCCCCATCGGACTCCGACCTG
CTCGGCCTGGCGCAGGACGAGATAGCGGAGTTCGAGGCCGAATTCGACGACGAACGTCAT
TCTCTGCGCTGA
[3] C13..308
[3] A487..877
[3] L-3,5-dihydroxyphenylglycine(Dpg)647..747
[3] PCP953..1022
[3] C37..924
[3] A1459..2631
[3] L-3,5-dihydroxyphenylglycine(Dpg)1939..2241
[3] PCP2857..3066

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[487-877] 1.60000000000002e-107 PF00501
PF00501   AMP-binding
IPR001242 Condensation domain (Domain)
[13-308] 9.9e-61 PF00668
PF00668   Condensation
IPR006162 Phosphopantetheine attachment site (PTM)
[981-996] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[953-1022] PS50075
PS50075   ACP_DOMAIN
[946-1021] 1.10000150671642e-17 SSF47336
SSF47336   ACP_like
[945-1022] 2.7e-23 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[956-1019] 1.4e-11 PF00550
PF00550   PP-binding
IPR010071 Amino acid adenylation domain (Domain)
[487-877] 1.69999999999998e-124 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020459 AMP-binding (Domain)
[596-607] 9.20000434662057e-05 PR00154 [608-616] 9.20000434662057e-05 PR00154
PR00154   AMPBINDING
IPR020845 AMP-binding, conserved site (Conserved_site)
[601-612] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
A4092_00310 A4092_00310   A4092_00330 A4092_00330
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