Biological Resource Center, NITE (NBRC)

Microbial Carotenoids

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Introduction

Since living organisms can produce many chemical substances in their metabolic systems, their functions can be maximally utilized to produce highly functional substances at high efficiency without the chemical synthesis process.
 Carotenoids are typical pigments produced by living organisms in their metabolic systems. They are well known as red or yellow pigments in tomatoes and carrots and as nutrients. Typical carotenoids include beta-carotene and astaxanthin. Some microorganisms also produce carotenoids.
 To enable the use of carotenoid-producing microorganisms, NITE has conducted advanced carotenoid measurements and analysis of carotenoid production and metabolism in microorganisms.
 

Chemical structural formula of typical carotenoids, beta-Carotene C40H56 Mol. Wt.:536.89 Exact Mass:536.4382, Astaxanthin C40H52O4 Mol. Wt.:596.85 Exact Mass:596.3866
 

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Analyzed Microorganisms

NITE selected 43 microorganisms such as yeasts and actinomycetes and analyzed the extracts by liquid chromatograph - mass spectrometry (LC/MS) to confirm whether they produced carotenoids and to identify the carotenoids they produced.
 The detailed analysis results are available to be downloaded from the DBRP (Data and Biological Resource Platform). (DBRP is only available in Japanese.)
 In the analysis result column of the table below, you can download photographs of the colony shapes from "Colony", images of the cell shapes from "Cell", nucleotide sequences from "Genome", predicted carotenoid-related gene sequences from "Gene Prediction", and LC/MS measurement results from "LC/MS", respectively.

 

No. Phylum Strain Analysis result
01 Actinobacteria Micrococcus luteus NBRC 3333 Colony
LC/MS
02 Actinobacteria Kocuria rosea NBRC 15588 LC/MS
03 Actinobacteria Kocuria flava NBRC 107626 Genome
LC/MS
04 Actinobacteria Streptomyces mirabilis NBRC 13450 LC/MS
05 Actinobacteria Streptomyces olivochromogenes NBRC 13067 Colony
LC/MS
06 Actinobacteria Actinophytocola gilvus NBRC 109453 Colony
Genome
Gene Prediction
LC/MS
07 Actinobacteria Actinoplanes teichomyceticus NBRC 13999 Colony
Genome
Gene Prediction
LC/MS
08 Actinobacteria Angustibacter luteus NBRC 105387 Colony
LC/MS
09 Actinobacteria Asanoa ishikariensis NBRC 14551 Colony
Genome
Gene Prediction
LC/MS
10 Actinobacteria Asanoa ferruginea NBRC 14496 Colony
Genome
Gene Prediction
LC/MS
11 Actinobacteria Cellulomonas aerilata NBRC 106308 Genome
Gene Prediction
LC/MS
12 Actinobacteria Nocardia seriolae NBRC 15557 Colony
Genome
LC/MS
13 Actinobacteria Streptoalloteichus hindustanus NBRC 15115 Colony
LC/MS
14 Actinobacteria Streptomyces cellostaticus NBRC 12849 LC/MS
15 Bacteroidetes Flavobacterium glycines NBRC 105008 Genome
Gene Prediction
LC/MS
16 Bacteroidetes Marinilabilia salmonicolor NBRC 15948 LC/MS
17 Firmicutes Bacillus vietnamensis NBRC 101237 Genome
Gene Prediction
LC/MS
18 Proteobacteria Corallococcus coralloides NBRC 100076 LC/MS
19 Proteobacteria Pseudomonas alcaligenes NBRC 14159 Genome
Gene Prediction
LC/MS
20 Proteobacteria Pseudomonas parafulva NBRC 16636 Genome
Gene Prediction
LC/MS
21 Proteobacteria Sphingomonas astaxanthinifaciens NBRC 102146 Genome
Gene Prediction
LC/MS
22 Proteobacteria Sphingomonas jaspsi NBRC 102120 Genome
Gene Prediction
LC/MS
23 Proteobacteria Sphingomonas pruni NBRC 15498 Genome
Gene Prediction
LC/MS
24 Proteobacteria Sphingomonas trueperi NBRC 100456 Genome
Gene Prediction
LC/MS
25 Proteobacteria Altererythrobacter ishigakiensis NBRC 107699 Genome
LC/MS
26 Proteobacteria Paracoccus marinus NBRC 100637 LC/MS
27 Rhodothermaeota Rubricoccus marinus NBRC 107124 Genome
LC/MS
28 (Bigyra) Aurantiochytrium sp. NBRC 102614 LC/MS
29 (Bigyra) Botryochytrium radiatum NBRC 104107 LC/MS
30 (Bigyra) Oblongichytrium sp. NBRC 102618 LC/MS
31 (Bigyra) Ulkenia amoeboidea NBRC 104106 LC/MS
32 (Bigyra) Aurantiochytrium sp. NBRC 111922 LC/MS
33 (Bigyra) Thraustochytriaceae sp. NBRC 111921 LC/MS
34 (Bigyra) Thraustochytrium sp. NBRC 111915 LC/MS
35 (Bigyra) Schizochytrium sp. NBRC 102617 LC/MS
36 Ascomycota Neonectria coccinea NBRC 104641 LC/MS
37 Mucoromycota Phycomyces blakesleeanus NBRC 33097 LC/MS
38 Mucoromycota Blakeslea trispora NBRC 32295 LC/MS
39 Mucoromycota Mucor circinelloides f. circinelloides NBRC 4554 LC/MS
40 Basidiomycota Rhodotorula toruloides NBRC 0559 Cell
LC/MS
41 Basidiomycota Rhodotorula aurantiaca NBRC 0754 Cell
Genome
Gene Prediction
LC/MS
42 Basidiomycota Rhodotorula mucilaginosa NBRC 0909 Genome
Gene Prediction
LC/MS
43 Basidiomycota Rhodotorula toruloides NBRC 10032 Genome
Gene Prediction
LC/MS
 

This study was sponsored by New Energy and Industrial Technology Development Organization (NEDO).

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Analytical Instruments

The mass spectrometer used for the analysis is introduced below.

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