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遺伝子 : Bifunctional polymyxin resistance protein ArnA (ARNA)

遺伝子情報

ID NRULE_0132
遺伝子ID ARNA
作成日 2013/02/12
タンパク質名
Protein name
Full
Bifunctional polymyxin resistance protein ArnA
遺伝子名 arnA
遺伝子条件 HAMAP; MF_01166; hit=1
参照
Gene Ontology
GO
GO:0050662, F:coenzyme binding, IEA:InterPro
GO:0016742, F:hydroxymethyl-, formyl- and related transferase activity, IDA:EcoCyc
GO:0008168, F:methyltransferase activity, IEA:UniProtKB-KW
GO:0000166, F:nucleotide binding, IEA:InterPro
GO:0016616, F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor, IDA:EcoCyc
GO:0009245, P:lipid A biosynthetic process, IDA:EcoCyc
GO:0009103, P:lipopolysaccharide biosynthetic process, IEA:UniProtKB-UniPathway
GO:0046677, P:response to antibiotic, IDA:EcoCyc
Phylogenomic databases
KO
K10011, -
Family and domain databases
HAMAP
MF_01166, ArnA, 1, -
InterPro
IPR021168, Bifun_polymyxin_resist_ArnA
IPR001509, Epimerase_deHydtase
IPR005793, Formyl_trans_C
IPR002376, Formyl_transf_N
Pfam
PF01370, Epimerase, 1
PF02911, Formyl_trans_C, 1
PF00551, Formyl_trans_N, 1
PIRSF
PIRSF036506, Bifun_polymyxin_resist_ArnA, 1
配列の特徴
P77398 Escherichia coli (strain K12).
NP_BIND368 - 369NAD binding.
ACT_SITE104Proton donor; for formyltransferase activity.
ACT_SITE434Proton acceptor; for decarboxylase activity.
ACT_SITE619Proton donor; for decarboxylase activity.
BINDING11410-formyltetrahydrofolate.
BINDING347NAD.
BINDING393UDP-glucuronate; via carbonyl oxygen.
BINDING398UDP-glucuronate.
BINDING460UDP-glucuronate.
BINDING492UDP-glucuronate.
BINDING613UDP-glucuronate.
SITE102Transition state stabilizer.
SITE140Raises pKa of active site His.
注釈
Function
Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto- arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4- amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido- arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity)
Catalytic activity
UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L- threo-pentapyranos-4-ulose + CO(2) + NADH
10-formyltetrahydrofolate + UDP-4-amino-4- deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4- formamido-beta-L-arabinose
Pathway
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido- beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L- arabinose from UDP-alpha-D-glucuronate: step 1/3
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido- beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L- arabinose from UDP-alpha-D-glucuronate: step 3/3
Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis
Subunit structure
Homohexamer, formed by a dimer of trimers (By similarity)
Sequence similarities
In the N-terminal section; belongs to the fmt family. UDP-L-Ara4N formyltransferase subfamily
In the C-terminal section; belongs to the sugar epimerase family. UDP-glucuronic acid decarboxylase subfamily
参考文献
P77398 Escherichia coli (strain K12).
11706007
Oxidative decarboxylation of UDP-glucuronic acid in extracts of polymyxin-resistant Escherichia coli. Origin of lipid a species modified with 4-amino-4-deoxy-L-arabinose. (J Biol Chem. 2002)
17928292
An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli. (J Biol Chem. 2007)



関与する機能

機能名
Polymyxin resistance

該当CDS

データダウンロード
学名 NBRC番号 CDS タンパク質名
Edwardsiella tarda105688ET1_02_00790UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronic acid oxidase
Enterobacter aerogenes13534EAE_05690bifunctional UDP-glucuronic acid decarboxylase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase
Enterobacter cloacae subsp. cloacae13535ECL_04861bifunctional UDP-glucuronic acid decarboxylase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase
Escherichia coli3972EcolC_1394NAD-dependent epimerase/dehydratase
Escherichia coli13500WFL_11970bifunctional UDP-glucuronic acid decarboxylase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase
Escherichia coli12713Y75_2258fused UDP-L-Ara4N formyltransferase and UDP-GlcA C-4'-decarboxylase
Escherichia fergusonii102419EFER_0914fused UDP-L-Ara4N formyltransferase ; UDP-GlcA C-4'-decarboxylase
Escherichia hermannii105704EH105704_01_08410bifunctional polymyxin resistance protein ArnA
Klebsiella pneumoniae14438KPR_5072
Klebsiella pneumoniae subsp. rhinoscleromatis105682HMPREF0484_0802bifunctional UDP-glucuronic acid decarboxylase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase
Proteus mirabilis105697HMPREF0693_0618methionyl-tRNA formyltransferase
Providencia alcalifaciens105687PROVALCAL_02523bifunctional UDP-glucuronic acid decarboxylase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase
Rahnella aquatilis105701Rahaq2_2851methionyl-tRNA formyltransferase
Shimwellia blattae105725EB105725_04_02080bifunctional polymyxin resistance protein ArnA
Yersinia rohdei105715yrohd0001_27820UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating