Rifam_00510 : CDS information

Location

Organism	Amycolatopsis mediterranei
Strain	S699
Entry name	Rifamycin
Contig	AF040570
Start / Stop / Direction	91,789 / 93,210 / + [in whole cluster] 91,789 / 93,210 / + [in contig]
Location	91789..93210 [in whole cluster] 91789..93210 [in contig]
Type	CDS
Length	1,422 bp (473 aa)

Annotation

Category	5.1 general function
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	putative 2,3-dehydratase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF18
Reference	ACC Q9AE03 PmId [16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005) comment PKS後修飾に関するgenesの報告。 orf18(putative 2,3-dehydratase)の不活化mutant作成あり。rifamycin B産生に影響しない。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment 20th(Q9ALN6) 51%, 1e-123 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [putative 2,3-dehydratase]
MVHSAIHEDTARDGRSDAVADDAVMSMADFHSWFNARARANRFQVDRIPFLELSGWDFEP
DTGNLVHASGRFFSVEGLRVRTDRPWVSEWAQPIIVQPEIGVLGILVKRFDGVPHCLMQA
KMEPGNINGLQISPTVQATRSNYMKVHGGAATKYMEYFRPGSRGRVLFDGLQSEQGSWFL
HKRNRNIVVETDDDVPLDEDFCWLTLDQIRRLLRLDHMVNMDSRTVLACVPPALARPDDR
TTGDGSFVEAVHRSLSGRGRAVHDPGHILSWLTEVRARQELVQRRVPLKEIVEDGWRLGH
DVIDHRDGKYFDVVAVAVEASNREVTAWTQPLVAPRQSGLLALLVKRIGGTLHALVQARS
DAGTLNIAELTATVHCQPGNYADAPAEYRPPYLDYALAAATGRVRLDVVHSEEGGRFYHA
QNRYLVIEVEDDFAAEPDDRFLWTTLNQLMSLLSHSNYLTVELRSLMAGMRSF

>putative NDP-hexose 2,3-dehydratase [putative 2,3-dehydratase]
GTGGTCCACAGCGCGATACACGAGGACACGGCACGGGACGGACGCTCCGACGCCGTGGCC
GACGACGCGGTCATGTCCATGGCGGACTTCCACTCCTGGTTCAACGCGCGAGCACGCGCG
AACCGCTTCCAGGTGGACCGGATTCCGTTCCTGGAGCTGTCCGGCTGGGACTTCGAGCCG
GACACGGGCAACCTGGTGCACGCCAGCGGCCGGTTCTTCTCGGTCGAGGGCCTGCGCGTC
CGGACCGACCGGCCCTGGGTGAGCGAGTGGGCCCAGCCGATCATCGTGCAGCCGGAGATC
GGCGTGCTCGGCATCCTCGTCAAGCGGTTCGACGGGGTCCCGCACTGCCTGATGCAGGCG
AAGATGGAGCCGGGCAACATCAACGGCCTGCAGATCTCGCCGACCGTGCAGGCCACGCGC
AGCAACTACATGAAGGTCCACGGCGGCGCGGCCACCAAGTACATGGAGTACTTCCGGCCC
GGCTCCCGCGGCCGCGTGCTGTTCGACGGGCTGCAGTCCGAGCAGGGGTCGTGGTTCCTG
CACAAGCGCAACCGCAACATCGTCGTCGAGACCGACGACGACGTCCCGCTGGACGAGGAC
TTCTGCTGGCTGACGCTCGACCAGATCCGGCGCCTGCTGCGGCTGGACCACATGGTGAAC
ATGGACTCCCGGACGGTTCTCGCGTGCGTCCCGCCCGCCTTGGCGCGGCCCGACGACCGG
ACGACCGGCGACGGCTCGTTCGTCGAAGCGGTCCACCGTTCGCTCTCGGGCCGCGGCCGG
GCCGTGCACGACCCGGGCCACATCCTCAGCTGGCTCACCGAGGTGCGCGCCCGCCAGGAG
CTGGTGCAGCGCCGGGTGCCGCTCAAGGAGATCGTCGAAGACGGCTGGCGGCTCGGTCAC
GACGTCATCGACCACCGCGACGGCAAGTACTTCGACGTCGTCGCGGTGGCCGTGGAAGCC
AGCAACCGCGAGGTCACCGCGTGGACCCAGCCGCTGGTGGCGCCCCGGCAGTCCGGCCTG
CTCGCGTTGCTGGTCAAGCGGATCGGCGGCACGCTGCACGCTCTGGTGCAGGCCCGCAGT
GACGCGGGCACGCTCAACATCGCCGAGCTCACCGCGACGGTGCACTGCCAGCCCGGCAAC
TACGCCGACGCGCCGGCGGAGTACCGCCCGCCCTACCTCGACTACGCGCTGGCCGCCGCC
ACCGGACGCGTCCGGCTGGACGTGGTGCATTCCGAGGAAGGCGGCCGGTTCTACCACGCG
CAGAACCGCTACCTGGTGATCGAGGTCGAGGACGACTTCGCCGCCGAGCCGGACGACCGG
TTCCTCTGGACCACGCTGAACCAGCTGATGTCGCTGCTGTCGCACAGCAACTACCTGACG
GTGGAACTGCGGAGCCTGATGGCGGGCATGCGCAGCTTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Rifam_00500 Rifam_00520