Rifam_00580 Rifam_00580   Rifam_00600 Rifam_00600

Rifam_00590 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction102,231 / 101,062 / - [in whole cluster]
102,231 / 101,062 / - [in contig]
Locationcomplement(101062..102231) [in whole cluster]
complement(101062..102231) [in contig]
TypeCDS
Length1,170 bp (389 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)putative cytochrome P450 monooxygenase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF16
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005)
comment
[PMID: 9512878](1998)
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
Orf16(351aa): cytochrom P450 monooxygenase

---
[PMID: 16079331](2005)
cytochrome P450候補ORF4つのinactivation mutantを作成し、表現型を解析。
orf16 mutantはrifamycins SV and Bの混合物を産生するので、orf16はglycolate side chainのformation and/or attachmentにある程度は関連するのかもしれない。

rifamycins SV and Bの違いは、ナフタレン環部分のhydroxyl基でのglycolate置換。
Related Reference
ACC
A6YRR3
PmId
[19376895] Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14. (Appl Environ Microbiol. , 2009)
comment
13th, id50%, 2e-89
Streptomyces virginiae
Cytochrome P450 monooxygenase 105

cytochrome P450 monooxygenase, FcpCを同定した文献。
FcpCがdiosgenoneのC-25のhydroxylationを担い、isonuatigenoneに変換する機能を持つことがin vitroで示されている。

この論文で同定されたfcpC(1191nt) and fcpD(243nt)遺伝子配列のGenBank accession numberはEF646279であると記載あり。
よってこのentry=FcpCで合っている。
ACC
D8HZB8
PmId
[20567260] Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism. (Cell Res. , 2010)
comment
blast search
Rifam_00590: 99.23%, 0.0

Amycolatopsis mediterranei (strain U-32)_AMED_0653
Cytochrome P450

[PMID:20567260](2010)
rifamycin SV産生株U32では、CYP450をコードするAMED_0653にR84Wのmissense変異あり。
これは他株のR84なCYP450で相補され、rifamycin SV → Bへの変換ができるようになる。

close this sectionSequence

selected fasta
>putative cytochrome P450 [putative cytochrome P450 monooxygenase]
MPPALVAVSERGPVARATLAAGDPFWLVSGYEEARAVLSDPRFSSDRFQYHPRFKELSPE
FRERLRDDKARAGSFINMDPPEHTRYRKLLTGQFTVRRIRELGARIDEIVAGRVDAMLAG
GTTADLMTEFAFPAPSLMICELLGVRYEDRAEFQQRASALLQMNAPVAEAVKNADALRAF
MQALVTDKRANPAGDIISGLIHHAGADPALTDDELINIANLLLIAGYDTTASMLGLGIFV
LLQRPAQLATLRDDPSRIADAVEGLLRYLSVVNPGIFRFAKEDLEFAGEHIPAGSTVVVS
VVATNRDARHWPDPDLDLTRPRGPHLAFGHGVHQCLGQQLARMEMQAGYAELLRRLPNVR
LAVPPEEVPLRNDMLTYGVHSLPIAWDAP
selected fasta
>putative cytochrome P450 [putative cytochrome P450 monooxygenase]
GTGCCGCCCGCTTTGGTGGCCGTCTCCGAACGGGGTCCCGTCGCCCGGGCGACACTGGCC
GCCGGCGACCCGTTCTGGCTCGTGTCCGGGTACGAGGAAGCGCGCGCGGTGCTGTCGGAC
CCGCGCTTTTCCTCCGACCGGTTCCAGTACCACCCGCGGTTCAAGGAACTGTCGCCCGAA
TTCCGGGAGCGCCTGCGGGACGACAAAGCGCGCGCGGGGTCGTTCATCAACATGGACCCG
CCGGAGCACACCCGGTACCGGAAACTGCTCACCGGGCAGTTCACCGTCCGGCGGATCCGC
GAGCTCGGCGCCCGGATCGACGAGATCGTCGCCGGCCGGGTGGACGCCATGCTGGCCGGC
GGGACCACCGCCGACCTGATGACCGAATTCGCGTTTCCGGCGCCTTCGCTGATGATCTGC
GAGCTGCTCGGGGTGCGGTACGAGGACCGGGCGGAGTTCCAGCAGCGCGCGTCGGCCCTG
CTGCAGATGAACGCCCCGGTCGCGGAGGCGGTGAAGAACGCCGACGCCCTGCGCGCGTTC
ATGCAGGCGCTGGTCACGGACAAGCGGGCGAACCCGGCGGGCGACATCATCTCCGGCCTG
ATCCACCACGCCGGCGCCGACCCCGCGCTCACCGACGACGAGCTGATCAACATCGCCAAC
CTGCTGCTCATCGCCGGCTACGACACCACGGCGAGCATGCTGGGGCTGGGCATCTTCGTG
CTGCTGCAGCGCCCCGCCCAGCTCGCCACGCTGCGCGACGACCCGTCCCGCATCGCCGAC
GCGGTCGAAGGACTGCTGCGCTACCTGTCCGTGGTGAACCCGGGGATCTTCCGCTTCGCC
AAGGAGGACCTGGAGTTCGCCGGCGAGCACATCCCGGCCGGTTCGACGGTGGTGGTCTCC
GTGGTGGCGACCAACCGCGACGCGCGGCACTGGCCGGACCCGGACCTCGACCTGACCCGC
CCCCGCGGCCCCCACCTGGCGTTCGGCCACGGCGTGCACCAGTGCCTCGGCCAGCAGCTG
GCCCGGATGGAGATGCAGGCGGGCTACGCCGAGCTGCTGCGCCGCCTGCCGAACGTTCGC
CTGGCGGTGCCGCCGGAGGAGGTTCCGCTGCGCAACGACATGCTGACTTACGGGGTGCAC
TCGCTTCCGATCGCCTGGGACGCTCCCTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[225-242] 3.79999862644791e-07 PR00385 [326-335] 3.79999862644791e-07 PR00385 [335-346] 3.79999862644791e-07 PR00385
PR00385   P450
[27-253] 2.1e-05 PF00067 [265-356] 3.7e-18 PF00067
PF00067   p450
[7-387] 2.70000000000002e-105 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[1-386] 1.19999063421924e-92 SSF48264
SSF48264   Cytochrome_P450
IPR002397 Cytochrome P450, B-class (Family)
[78-89] 6.70002630158883e-47 PR00359 [125-141] 6.70002630158883e-47 PR00359 [142-157] 6.70002630158883e-47 PR00359 [179-201] 6.70002630158883e-47 PR00359 [260-271] 6.70002630158883e-47 PR00359 [278-305] 6.70002630158883e-47 PR00359 [326-335] 6.70002630158883e-47 PR00359 [335-346] 6.70002630158883e-47 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[328-337] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Rifam_00580 Rifam_00580   Rifam_00600 Rifam_00600
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