Rifam_00590 Rifam_00590   Rifam_00610 Rifam_00610

Rifam_00600 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction102,476 / 102,967 / + [in whole cluster]
102,476 / 102,967 / + [in contig]
Location102476..102967 [in whole cluster]
102476..102967 [in contig]
TypeCDS
Length492 bp (163 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative aminodehydroquinate dehydratase
Product (GenBank)aminodehydroquinate dehydratase
Gene
Gene (GenBank)rifJ
EC number
Keyword
  • AHBA
Note
Note (GenBank)
  • RifJ
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
comment
[PMID: 9512878](1998)
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
RifJ(163aa): aminodehydroquinate dehydratase

RifJは、rifamycin PKSのstarter unitであるAHBAの合成過程で、aDHQ→aDHSを触媒すると考えられている。

aDHQ: 5-deoxy-5-amino-3-dehydroquinate
aDHS: 5-deoxy-5-amino-3-dehydroshikimate

---
[PMID: 11278540](2001)
rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

RifJ: aminodehydroquinate dehydratase

rifH, -G, and -J genesの遺伝子産物は shikimate biosynthesis pathwayに関連する酵素に似ている。rifH, G, Jの各mutantは成長に影響を与えないので、shikimate pathwayに直接関連しないことがわかる。また、mutantでもrifamycin B産生をある程度保つことから、shikimate pathwayのgeneが代替していると考えられる。
Related Reference
ACC
P43877
PmId
[8170389] Characterization of a 3-dehydroquinase gene from Actinobacillus pleuropneumoniae with homology to the eukaryotic genes qa-2 and QUTE. (Mol Microbiol. , 1994)
comment
28th, id48, 6e-31
Actinobacillus pleuropneumoniae_aroQ
3-dehydroquinate dehydratase(EC 4.2.1.10)

HAMAP MF_00169 template

大腸菌aroD(classII)を相補するものとしてクローニングされた。aroDとは相同性がない。

close this sectionSequence

selected fasta
>putative aminodehydroquinate dehydratase [aminodehydroquinate dehydratase]
MVQAPCRTRCVPLPDVLLLNGPNLGVLGRREPEIYGTDTLADIEKAVAEEVRPRGWTVVP
VQRDGEGELVGAIHQHRDTTVGAIVNPGALMIAGWSLRDALASYEPPWIEVHLSNVWARE
QFRHDSVIAPLASGVVVGLGAFGYRLAAQALLHLSAESPNGLR
selected fasta
>putative aminodehydroquinate dehydratase [aminodehydroquinate dehydratase]
GTGGTCCAGGCCCCCTGCCGAACGAGGTGTGTCCCGTTGCCCGACGTCCTGCTGCTGAAC
GGTCCCAACCTGGGTGTGCTGGGCCGGCGCGAGCCCGAGATCTACGGCACCGACACGCTC
GCCGACATCGAAAAGGCGGTCGCCGAGGAGGTGCGGCCCCGCGGCTGGACCGTCGTCCCG
GTCCAGCGCGACGGCGAGGGCGAGCTGGTCGGCGCGATCCACCAGCACCGCGACACGACG
GTCGGCGCGATCGTCAACCCCGGTGCGCTGATGATCGCCGGCTGGAGCCTGCGCGACGCG
CTGGCCAGCTACGAGCCGCCGTGGATCGAGGTCCACCTGAGCAACGTGTGGGCGCGCGAG
CAGTTCCGGCACGACTCGGTCATCGCCCCGCTGGCGAGCGGGGTGGTGGTCGGCCTCGGC
GCCTTCGGCTACCGCCTGGCCGCACAGGCGTTGCTGCACCTGTCCGCCGAATCGCCGAAT
GGGCTCCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001874 Dehydroquinase, class II (Family)
[14-156] 2.89999999999999e-54 G3DSA:3.40.50.9100
G3DSA:3.40.50.9100   DHquinase_II
[12-157] 3.19999899046355e-65 PIRSF001399
PIRSF001399   DHquinase_II
[15-152] 4.6e-49 PF01220
PF01220   DHquinase_II
[8-156] 3.49999466863949e-49 SSF52304
SSF52304   DHquinase_II
[29-160] PD004527
PD004527   DHquinase_II
IPR018509 Dehydroquinase, class II, conserved site (Conserved_site)
[19-36] PS01029
PS01029   DEHYDROQUINASE_II
SignalP No significant hit
TMHMM
[126-148] Transmembrane (i-o)
Transmembrane 1   
Rifam_00590 Rifam_00590   Rifam_00610 Rifam_00610
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