Urd_00060 : CDS information

Location

Organism	Streptomyces fradiae
Strain	Tü2717
Entry name	Urdamycin
Contig	AF164961
Start / Stop / Direction	7,262 / 8,437 / + [in whole cluster] 7,262 / 8,437 / + [in contig]
Location	7262..8437 [in whole cluster] 7262..8437 [in contig]
Type	CDS
Length	1,176 bp (391 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	L-rhodinose glycosyltransferase
Product (GenBank)	glycosyl transferase
Gene
Gene (GenBank)	urdGT1c
EC number
Keyword	C-9 trisaccharide 2nd L-rhodinose
Note
Note (GenBank)	UrdGT1c
Reference	ACC Q9RP99 PmId [10662691] Function of glycosyltransferase genes involved in urdamycin A biosynthesis. (Chem Biol. , 2000) [11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000) [11410375] Two sequence elements of glycosyltransferases involved in urdamycin biosynthesis are responsible for substrate specificity and enzymatic activity. (Chem Biol. , 2001) comment UniProtで登録されている[PMID: 10658661](2000)に、urdGT1cについての記載はない。 --- [PMID: 10662691](2000) [PMID: 11094336](2000) urdamycin clusterに含まれるglycosyltransferase候補genes=urdGT1a, urdGT1b, urdGT1cについて、mutant作成、相補実験における蓄積産物解析を行い、各機能割り当てが確定されている。 UrdGT1cは、urdamycin A生合成においてC-9での3糖側鎖2番目にあるL-rhodinoseの付着を担うO-glycosyltransferaseである。 3糖側鎖3番目にL-rhodinoseを持つurdamycin O, urdamycin Nの形成にも関与する。 urdamycin AなどはC-12bにも同じL-rhodinoseが付着するが、これを担うのはUrdGT1aであり、UrdGT1cは代替しない。 -- [PMID: 11410375](2001) アミノ酸を91%共有しているUrdGT1b and UrdGT1cの基質特異性の違いに関する検討。 nucleotide sugarとacceptor substrateの特異性を決定するのはN末である。

Sequence

>L-rhodinose glycosyltransferase [glycosyl transferase]
MRVLFLVTPSPTHFTPLVSLAWALRGAGHEVVVAGQPDVLGAVASAGLNAVSIGAPFNGE
AKLLAGLGPDQRPLEVRPRPAPESMGGYGRVWMTHARYLVGRYMEFARIYGPDLIVSDPL
EYSSLLVGGVLGVPVVQQRWGVDLISGPARAEARPGFGPLCERLGLAGLPDPAVLLDPCP
PGLQAPGAEPGSPIRFVPFNGNGVVPGWLREPRSASRPRVLVTIGGTLALNGVPLMRGIL
RAFEELPEVEAVATVDEVFREKVGPVPANVRMVDPVPLHLVLDGCAAVVHHGGAGTTMTA
GAFGLPQLVLPQLADHFGHGDRVSEVGAGISLDDAESQNDSHRLAVELRRLLAEPEFAKA
ARALADSVRDMPAPAQVAADLTRIAGVVGAL

>L-rhodinose glycosyltransferase [glycosyl transferase]
ATGCGAGTCCTGTTTCTGGTGACACCCTCGCCGACGCATTTCACGCCGTTGGTGTCATTG
GCGTGGGCCCTGCGGGGTGCGGGCCATGAGGTGGTGGTCGCGGGTCAGCCGGATGTACTG
GGGGCGGTCGCCTCGGCCGGGCTCAACGCGGTGAGCATCGGCGCACCGTTCAACGGGGAG
GCGAAGCTGCTCGCCGGACTGGGCCCCGATCAGCGGCCGCTGGAGGTACGGCCCCGCCCG
GCGCCTGAGTCGATGGGCGGGTACGGGCGGGTGTGGATGACCCATGCGCGTTATCTGGTC
GGGCGGTACATGGAGTTCGCTCGCATCTACGGTCCGGATCTGATCGTGTCGGATCCGCTG
GAGTACAGCTCGCTGCTGGTCGGGGGTGTGCTGGGGGTTCCGGTGGTGCAGCAGCGGTGG
GGGGTGGATCTGATCTCGGGTCCGGCGCGTGCGGAGGCGCGGCCGGGGTTCGGTCCGTTG
TGTGAGCGTCTGGGGCTTGCGGGGCTGCCGGATCCGGCGGTGTTGCTGGATCCGTGTCCG
CCGGGTCTGCAGGCTCCGGGGGCGGAGCCGGGCAGTCCGATCAGGTTCGTTCCGTTCAAC
GGCAACGGGGTGGTGCCGGGGTGGCTGCGCGAGCCGCGGTCCGCATCACGCCCCCGTGTA
CTCGTCACCATCGGTGGCACGTTGGCGTTGAACGGGGTGCCGTTGATGCGGGGCATTCTG
CGGGCGTTCGAGGAGCTGCCGGAGGTGGAGGCGGTGGCGACGGTGGACGAGGTGTTCCGG
GAGAAGGTCGGGCCGGTGCCGGCCAATGTGCGGATGGTCGATCCGGTGCCGCTGCACCTG
GTGCTGGACGGATGTGCGGCCGTTGTGCACCACGGGGGCGCGGGTACGACGATGACCGCC
GGGGCGTTCGGGCTGCCCCAGCTGGTACTGCCCCAGCTCGCCGACCACTTCGGGCACGGT
GACCGGGTGAGCGAGGTGGGTGCCGGGATCTCGCTGGACGATGCGGAGAGCCAGAACGAT
TCGCATCGGCTGGCCGTGGAACTTCGGCGGCTGTTGGCGGAGCCGGAGTTCGCCAAGGCC
GCCCGCGCGCTGGCCGACTCGGTCCGGGACATGCCCGCACCCGCACAGGTCGCCGCCGAC
CTCACACGCATCGCGGGTGTCGTCGGCGCCCTCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	No significant hit

Urd_00050 Urd_00070