Gelda_00170 Gelda_00170   Gelda_00150 Gelda_00150

Gelda_00160 : CDS information

close this sectionLocation

Organism
Strain17997
Entry nameGeldanamycin
Contig
Start / Stop / Direction6,661 / 6,029 / - [in whole cluster]
2,241 / 2,873 / + [in contig]
Locationcomplement(6029..6661) [in whole cluster]
2241..2873 [in contig]
TypeCDS
Length633 bp (210 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative RNA polymerase ECF-type sigma factor
Product (GenBank)putative ECF-family sigma factor
Gene
Gene (GenBank)gdnS
EC number
Keyword
Note
Note (GenBank)
  • GdnS
Reference
ACC
PmId
[16502293] Identification of AHBA biosynthetic genes related to geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Curr Microbiol. , 2006)
comment
S. hygroscopicus 17997のcosmid libraryをスクリーニングしたところ、2つのlociに分離したAHBA生合成gene clustersが発見された。このclustersは配列相同性と遺伝子編成の比較からbenzenic AHBA gene cluster(gdn)とnaphthalenic AHBA gene cluster(shn)に分けられた。

当ORF(Fig. 1.(D)のun)を含むgdn cluster deleted mutantはgeldanamycin収量が75%減った。
最も保存されているgdnA, -O, -Pのdeleted mutantでもgeldanamycin収量が75%減った。
Related Reference
ACC
P66809
PmId
[15049808] ( , )
comment
Blast id49%, 3e-30
Mycobacterium tuberculosis_sigC
ECF RNA polymerase sigma factor SigC

---
[PMID:10027986](1999)
sigma factor genes sigA, B, C, D, E, F, H, I, Mの発現解析。

sigma-C (Rv2069) : ECF subfamily sigma factor

---
[PMID:15049808](2004)
sigCを欠いたmutantは有意に弱毒化され、感染したマウスが死ななくなった。
SigCは病原性関連遺伝子の発現を調節することをマイクロアレイと定量的RT-PCR解析で確認。
ACC
Q49BB8
NITE
Gelda2_00310
comment
Blast id90%, 2e-96
geldanamycin clusterを持つNRRL 3602株のORF。

gdm PKS clusterから30kb以上離れたところにあるAHBA関連clusterに隣接するORF。
UniProtで登録されている[PMID:16085885]で同定されたという6ORFsにこのORFは含まれない。

close this sectionSequence

selected fasta
>putative RNA polymerase ECF-type sigma factor [putative ECF-family sigma factor]
MPSATLPAAPIRAVHGLATAANDHQVTEWALAARDGDREAVDHFIRATYRDVRRFVLHLS
ADPHGCEDLAQETYLRALTGLPRFAGRSSARTWLLSIARRVVVDRYRTAAARPRTLDADD
WQEAAERAQPAGLPGFDEGVALMDLLAALAPARREMFLLTKVLGLPYADAATATGCPIGT
VRSRVARAREDISALLAAAEKAAGPVPLVG
selected fasta
>putative RNA polymerase ECF-type sigma factor [putative ECF-family sigma factor]
ATGCCTTCTGCCACGCTGCCCGCCGCACCGATAAGAGCCGTGCACGGCCTTGCCACCGCG
GCGAACGACCACCAGGTCACCGAATGGGCGCTGGCCGCCCGGGACGGCGACCGCGAGGCG
GTCGACCACTTCATCCGCGCCACCTACCGCGATGTGCGCCGTTTCGTGCTCCACCTCAGC
GCCGATCCGCATGGTTGTGAGGACCTCGCCCAGGAGACGTATCTGCGGGCGCTGACCGGG
CTGCCGCGCTTCGCCGGTCGCTCATCGGCCCGGACGTGGCTGCTGTCGATCGCCCGCCGT
GTGGTCGTCGACCGCTACCGCACGGCCGCCGCCCGTCCCCGTACGTTGGACGCGGACGAC
TGGCAGGAGGCGGCCGAACGGGCGCAGCCCGCCGGGCTCCCCGGGTTCGACGAGGGGGTG
GCGCTGATGGACCTGCTGGCGGCGCTCGCCCCGGCACGCCGTGAGATGTTCCTCCTCACC
AAGGTGCTCGGCCTGCCGTACGCGGACGCCGCGACCGCGACCGGCTGCCCCATCGGCACC
GTACGCTCGCGCGTGGCCCGCGCCCGTGAGGACATCTCCGCGCTGCTGGCCGCGGCCGAG
AAGGCCGCGGGACCGGTGCCGTTGGTGGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR007627 RNA polymerase sigma-70 region 2 (Domain)
[45-110] 2e-14 PF04542
PF04542   Sigma70_r2
IPR013249 RNA polymerase sigma factor 70, region 4 type 2 (Domain)
[141-191] 3.5e-14 PF08281
PF08281   Sigma70_r4_2
IPR013324 RNA polymerase sigma factor, region 3/4 (Domain)
[131-198] 6.899999633797e-10 SSF88659
SSF88659   Sigma_r3_r4
IPR013325 RNA polymerase sigma factor, region 2 (Domain)
[18-130] 2.70000183580794e-22 SSF88946
SSF88946   Sigma_r2
IPR014284 RNA polymerase sigma-70 (Domain)
[39-193] 1.2e-20 TIGR02937
TIGR02937   Sigma70-ECF
SignalP No significant hit
TMHMM No significant hit
Gelda_00170 Gelda_00170   Gelda_00150 Gelda_00150
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