Biological Resource Center, NITE (NBRC)

Human GatewayTM Entry Clones


Announcement of the termination of DNA resources distribution services.

Human GatewayTM Entry Clones

The following kinds of clone are available.

Here is the information about "Human GatewayTM Entry Clones". [short cut to how to order].

*GatewayTM is a registered trademark of Thermo Fisher Scientific Inc.

About Human GatewayTM Entry Clones

Human Gateway entry clone

The human GatewayTM entry clones were constructed by cloning of PCR-amplified open reading frame (ORF) fragments from full-length human cDNA clones or other resource cDNAs and studied in the "NEDO Functional Analysis of Protein and Research Application Project" supported by the Japanese government. As of June 2010, about 43,000 human GatewayTM entry clones were deposited to NBRC by Japan Biological Informatics Consortium (JBIC) as a general deposit.

The results of this project using these human GatewayTM entry clones were published on Nature Methods and other journals. Furthermore, the detail information of these clones are available from H-Invitational Database (H-InvDB; and Human Gene and Protein Database (HGPD;

The DNA fragment in a GatewayTM entry clone can be moved into one or more destination vectors simultaneously by GatewayTM technology. For more information about GatewayTM technology, please visit the website of Thermo Fisher Scientific (


  1. 1.  Goshima, N. et al, Human protein factory for converting the transcriptome into an in vitro-expressed proteome., Nature Methods, 5, 1011 - 1017 (2008) [PubMed: 19054851]
  2. Maruyama, Y. et al, Human Gene and Protein Database (HGPD): a novel database presenting a large quantity of experiment-based results in human proteomics., Nucleic Acids Res, 37, D762-6 (2009) [PubMed: 19073703]

Clone Information

Click here for more information (clone map and vector sequence and so on) about human GatewayTM entry clones.

Clones used in the iPS cell Research

Click here for the list including Glis 1 clones .

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Depending on the culture, preservation conditions, judicial regulations, etc., DNA resource(s) will be either removed from the distribution list or modified for their distribution conditions without prior notice.

As stated in the "Terms and conditions" mentioned above, the DNA resource(s) you have received from us should not be transferred to a third party.

In order to improve our resources, we would like you to indicate the following items in your publication:

  1. 1.  The accession number of human GatewayTM entry clone(s); and
  2. 2.  "NBRC, NITE, Kisarazu, Japan" as the source of the clone.

Also, we appreciate receiving reprints of your publications as well as any information concerning your research using the human GatewayT entry clone(s) NBRC has supplied. We do not disclose your personal information to any third parties. If you have any questions concerning our DNA resources and/or related matters, please send an e-mail to: We cannot answer to your questions by telephone.

Contact us

 Culture Collection Division,  Biological Resource Center,  National Institute of Technology and Evaluation
Phone number:+81-438-20-5763
Address:2-5-8 Kazusakamatari, Kisarazu-shi, Chiba 2920818, Japan MAP
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