Biological Resource Center, NITE (NBRC)

Human cDNA Clones

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Announcement of the termination of DNA resources distribution services.

The following kinds of clone are available.

Here is information about "Full-length Human cDNA Clones" and "Splicing-Variant Human cDNA Clones". [short cut to how to order].

*GatewayTM is a registered trademark of Thermo Fisher Scientific Inc.

About Human cDNA Clones

Human cDNA clones were prepared at the Institute of Medical Science of the University of Tokyo (IMS-UT) and Helix Research Institute, Inc (HRI) by the "oligo-capping" method [gif:24KB]1, 2. And at Kazusa DNA Research Institute (KDRI) the cDNAs were also generated by conventional methods and strictly size-selected.

These clones were provided and analyzed in the following two projects;

NEDO Full-length human cDNA sequencing project

This project was performed by Research Association for Biotechnology (RAB) and supported by the New Energy and Industrial Technology Development Organization (NEDO). It was carried out from 1998 till 2002, in a transition period from the structural to functional genomics. The project has already been completed and resulted in more than 30,000 Full-length human cDNA clones from various human organs. Currently, RAB administers cDNA clones from IMS-UT and HRI independently. The clones were deposited to NBRC and NBRC has started distribution of them since 2002.

NEDO Functional analysis of protein and research application project

"Human cDNA sequencing project" focused on splicing variants of mRNA in this project supported by the Japanese government was carried out from 2002 till 2006 and has resulted in about 25,000 Splicing-varinat human cDNA clones. These clones were deposited to NBRC and NBRC has started the distribution since 2007.

Please refer FAQ for more details.

Vector Information

pME18SFL3 (3,394 bp) Show Sequence File [Word:35KB]
pUC19FL3 (3,098 bp) Show Sequence File [Word:36KB]

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Reference

Maruyama K, Sugano S, Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides., Gene, 38(1-2), 171-4 (1994) [PubMed: 8125298]

Suzuki et al., Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library., Gene, 200(1-2), 149-56 (1997) [PubMed: 9373149]

Related Links

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Notice

Depending on the culture, preservation conditions, judicial regulations, etc., DNA resource(s) will be either removed from the distribution list or modified for their distribution conditions without prior notice.

As stated in the "Terms and conditions" mentioned above, the DNA resource(s) you have received from us should not be transferred to a third party.

In order to improve our resources, we would like you to indicate the following items in your publication:

  1. 1.  The accession number of human cDNA clone(s); and
  2. 2.  "NBRC, NITE, Kisarazu, Japan" as the source of the clone.

Also, we appreciate receiving reprints of your publications as well as any information concerning your research using the human cDNA clone(s) NBRC has supplied.

We do not disclose your personal information to any third parties. If you have any questions concerning our DNA resources and/or related matters, please send an e-mail to: dna@nite.go.jp. We cannot answer to your questions by telephone.

Please refer FAQ for more details.

 

Contact us

Biological Resource Center (NBRC),
National Institute of Technology and Evaluation
2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN

FAX:
+81-438-52-2329
E-mail:
dna@nite.go.jp

Contact us

 Culture Collection Division,  Biological Resource Center,  National Institute of Technology and Evaluation
Phone number:+81-438-20-5763
Fax number:+81-438-52-2329
Address:2-5-8 Kazusakamatari, Kisarazu-shi, Chiba 2920818, Japan MAP
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