Rule : Rubredoxin reductase (RUBB)

Rule Description

NRULE_0040

Rule name

RUBB

Created

2012-09-07

Protein name

Gene name

rubB

Rule Trigger

BLASTP; Q0VTB0; hit=1; score=639; pid=38

BLASTP; Q93DN0; hit=1; score=770; pid=41

BLASTP; P17052; hit=1; score=750; pid=40

Cross Reference

Gene Ontology

GO: GO:0050660, F:flavin adenine dinucleotide binding, IEA:InterPro; GO:0015044, F:rubredoxin-NAD+ reductase activity, IEA:EC; GO:0043448, P:alkane catabolic process, IMP:UniProtKB

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

InterPro: IPR013027, FAD_pyr_nucl-diS_OxRdtase; IPR023753, Pyr_nucl-diS_OxRdtase_FAD/NAD; IPR001327, Pyr_OxRdtase_NAD-bd_dom
Pfam: PF00070, Pyr_redox, 1; PF07992, Pyr_redox_2, 1
PRINTS: PR00368, FADPNR

Feature

P17052 Pseudomonas oleovorans.

BINDING	41	FAD or NAD.
BINDING	79	FAD or NAD; viaamide nitrogen and carbonyl oxygen.
BINDING	156	FAD or NAD.

General annotation

Function: Involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1-monooxygenase
Catalytic activity: 2 reduced rubredoxin + NAD(+) + H(+)= 2 oxidized rubredoxin + NADH
Cofactor: FAD
Pathway: Hydrocarbon metabolism; alkane degradation
Subunit structure: Homodimer
Sequence similarities: Belongs to the FAD-dependent oxidoreductase family

Reference

P17052 Pseudomonas oleovorans.

2319593: Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. (J Mol Biol. 1990)

Q0VTB0 Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 SK2).

21714118: Rubredoxin reductase from Alcanivorax borkumensis: expression and characterization. (Biotechnol Prog. 2011)

Q93DN0 Rhodococcus sp. (strain Q15).

12450813: Gene cloning and characterization of multiple alkane hydroxylase systems in Rhodococcus strains Q15 and NRRL B-16531. (Appl Environ Microbiol. 2002)

Function name
n-alkane hydroxylation


Scientific name	NBRC No.	CDS	Product name
Acinetobacter radioresistens	102413	ACRAD_02_01990	rubredoxin reductase
Acinetobacter sp.	100985	ACT4_013_00270	rubredoxin-NAD(+) reductase
Congregibacter litoralis	104960	KT71_05015	NAD(P)H-nitrite reductase
Halomonas elongata	15536	HELO_4414	FAD-dependent pyridine nucleotide-disulphide oxidoreductase
Magnetospirillum magnetotacticum	15272	Magn03010384	COG0446: Uncharacterized NAD(FAD)-dependent dehydrogenases
Nitrosomonas europaea	14298	NE1420	probable rubredoxin reductase
Pseudomonas putida	100650	PP_5314	rubredoxin reductase
Pseudomonas putida	100650	PP_5371	rubredoxin/rubredoxin reductase
Pseudomonas stutzeri	14165	PSTAB_0469	rubredoxin reductase
Rhodococcus erythropolis	100887	RER_07490	rubredoxin reductase
Rhodococcus opacus	108011	ROP_22600	rubredoxin reductase
Rhodococcus opacus	108011	ROP_pROB01-04380	rubredoxin reductase
Tsukamurella paurometabola	16120	Tpau_2747	FAD-dependent pyridine nucleotide-disulfide oxidoreductase