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遺伝子 : 2,4-dihydroxyhept-2-ene-1,7-dioate aldolase (HPAI)

遺伝子情報

ID NRULE_0199
遺伝子ID HPAI
作成日 2013/01/18
タンパク質名
Protein name
Full
2,4-dihydroxyhept-2-ene-1,7-dioate aldolase
Short
HHED aldolase
EC
EC 4.1.2.-
Synonym
Full
4-hydroxy-2-oxo-heptane-1,7-dioate aldolase
遺伝子名 hpcH, hpaI
遺伝子条件 TIGRFAMs; TIGR02311; hit=1
参照
Gene Ontology
GO
GO:0018802, F:2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity, IEA:InterPro
GO:0016832, F:aldehyde-lyase activity, IEA:EC
GO:0046872, F:metal ion binding, IEA:UniProtKB-KW
GO:0010124, P:phenylacetate catabolic process, IEA:InterPro
Phylogenomic databases
KO
K02510, -
Enzyme and pathway databases
UniPathway
UPA00208, UER00422
Family and domain databases
HAMAP
MF_01292, HKHD_aldolase, 1, -
InterPro
IPR005000, Aldehyde-lyase_domain
IPR023701, HKHD_aldolase_ent
IPR012689, HpaI
IPR015813, Pyrv/PenolPyrv_Kinase
Pfam
PF03328, HpcH_HpaI, 1
TIGRFAMs
TIGR02311, HpaI, 1
配列の特徴
Q47098 Escherichia coli.
ACT_SITE45Proton acceptor.
METAL149Divalent metal cation.
METAL175Divalent metal cation.
BINDING147Substrate.
BINDING174Substrate; via amide nitrogen.
BINDING175Substrate.
注釈
Function
Catalyzes the reversible retro-aldol cleavage of 2,4-dihydroxyhept-2-ene-1,7-dioate aldolase (HHED) to pyruvate and succinate semialdehyde
Catalytic activity
2,4-dihydroxyhept-2-ene-1,7-dioate aldolase = pyruvate + succinate semialdehyde
Cofactor
Binds 1 divalent metal cation per subunit
Pathway
Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 7/7
Subunit structure
Homohexamer; trimer of dimers
Sequence similarities
Belongs to the HpcH/HpaI aldolase family
参考文献
Q47098 Escherichia coli.
8529896
Sequence of the Escherichia coli C homoprotocatechuic acid degradative operon completed with that of the 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase-encoding gene (hpcH). (Gene. 1995)
16511168
Expression, purification and preliminary crystallographic analysis of 2,4-dihydroxy-hepta-2-ene-1,7-dioate aldolase (HpcH) from Escherichia coli C. (Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005)
15996099
Purification and biochemical characterization of a pyruvate-specific class II aldolase, HpaI. (Biochemistry. 2005)



関与する機能

機能名
4-hydroxyphenylacetate degradation

該当CDS

データダウンロード
学名 NBRC番号 CDS タンパク質名
Edwardsiella tarda105688ET1_06_018102-keto-3-deoxy-L-rhamnonate aldolase
Enterobacter aerogenes13534EAE_103302,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Enterobacter hormaechei105718HMPREF9086_18912,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Escherichia coli3972EcolC_37072,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Escherichia coli13500WFL_229352,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Escherichia fergusonii102419EFER_30672,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Frateuria aurantia3245Fraau_17192,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Klebsiella pneumoniae14438KPR_0862
Klebsiella pneumoniae subsp. rhinoscleromatis105682HMPREF0484_50942,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Labrenzia aggregata16684SIAM614_21822HpcH/HpaI aldolase
Magnetospirillum magnetotacticum15272Magn03009777COG3836: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Providencia alcalifaciens105687PROVALCAL_003272,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Rhizobium etli15573RHE_CH023002,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase protein (4-hydroxyphenylacetate catabolism protein)
Rhizobium sp.101917NGR_b059102,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
NGR_b201702,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Rhizobium tropici15247RTCIAT899_PC038352,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
RTCIAT899_PC079702,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Serratia odorifera102598HMPREF0758_06522,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Shimwellia blattae105725EB105725_26_00180putative 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Sphingomonas wittichii105917Swit_30872,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Swit_42702,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase