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Rule : Protocatechuate 3,4-dioxygenase alpha subunit (PCAG)

Rule Description

ID NRULE_0066
Rule name PCAG
Created 2012-10-05
Protein name
Protein name
Full
Protocatechuate 3,4-dioxygenase alpha subunit
EC
EC 1.13.11.3
Gene name pcaG
Rule Trigger TIGRFAMs; TIGR02423; hit=1
Cross Reference
Gene Ontology
GO
GO:0008199, F:ferric iron binding, IEA:InterPro
GO:0018578, F:protocatechuate 3,4-dioxygenase activity, IEA:EC
GO:0042952, P:beta-ketoadipate pathway, IEA:UniProtKB-UniPathway
Phylogenomic databases
eggNOG
COG3485, -
KO
K00448, -
ProtClustDB
CLSK2307424, -
Enzyme and pathway databases
UniPathway
UPA00157, UER00264
Family and domain databases
InterPro
IPR000627, Intradiol_dOase_C
IPR015889, Intradiol_dOase_core
IPR012786, Protocat_dOase_a
Pfam
PF00775, Dioxygenase_C, 1
TIGRFAMs
TIGR02423, protocat_alph, 1
PROSITE
PS00083, INTRADIOL_DIOXYGENAS, 1
Feature
P00436 Pseudomonas putida (Arthrobacter siderocapsulatus).
BINDING134Protocatechuate.
General annotation
Function
Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway
Catalytic activity
3,4-dihydroxybenzoate + O(2) = 3-carboxy- cis,cis-muconate
Cofactor
Binds Fe(3+) ion per subunit
Pathway
Aromatic compound metabolism; beta-ketoadipate pathway; 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate: step 1/1
Subunit structure
The enzyme is an oligomer of 12 copies of the alpha and beta chains
Sequence similarities
Belongs to the intradiol ring-cleavage dioxygenase family
Reference
P20371 Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1).
2298704
DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence. (J Bacteriol. 1990)
P00436 Pseudomonas putida (Arthrobacter siderocapsulatus).
9254600
Crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase: endogenous Fe3+ ligand displacement in response to substrate binding. (Biochemistry. 1997)
Q0SH26 Rhodococcus jostii (strain RHA1).
25558786
In vitro reconstitution of the catabolic reactions catalyzed by PcaHG, PcaB, and PcaL: the protocatechuate branch of the beta-ketoadipate pathway in Rhodococcus jostii RHA1. (Biosci Biotechnol Biochem. 2015)
Q9ZFA0 Streptomyces sp. 2065.
10742233
Characterization of the protocatechuic acid catabolic gene cluster from Streptomyces sp. strain 2065. (Appl Environ Microbiol. 2000)



Related Function

Function name
Protocatechuate degradation (beta-ketoadipate pathway)

CDS

Data Download
Scientific name NBRC No. CDS Product name
Acidiphilium multivorum100883ACMV_20890protocatechuate 3,4-dioxygenase alpha subunit
Acinetobacter sp.100985ACT4_036_01030protocatechuate 3,4-dioxygenase alpha subunit
Actinoplanes missouriensis102363AMIS_36680putative protocatechuate 3,4-dioxygenase alpha subunit
Actinopolyspora iraqiensis103187ACTIR_613_00020protocatechuate 3,4-dioxygenase, alpha subunit
Actinosynnema mirum14064Amir_5084protocatechuate 3,4-dioxygenase, alpha subunit
Amycolatopsis azurea14573C791_0886Protocatechuate 3,4-dioxygenase alpha chain
Amycolatopsis mediterranei13415RAM_09445protocatechuate 3,4-dioxygenase, alpha subunit
Arthrobacter globiformis12137ARGLB_036_00400protocatechuate 3,4-dioxygenase alpha subunit
Azorhizobium caulinodans14845AZC_1442protocatechuate 3,4-dioxygenase alpha chain protein
Bradyrhizobium diazoefficiens14792blr0928protocatechuate 3,4-dioxygenase alpha chain
blr2334protocatechuate 3,4-dioxygenase alpha chain
Brevibacterium linens12170BlinB_010200001672protocatechuate 3,4-dioxygenase, alpha subunit
Candidatus Solibacter usitatusAcid_0246protocatechuate 3,4-dioxygenase, alpha subunit
Catenulispora acidiphila102108Caci_4889protocatechuate 3,4-dioxygenase, alpha subunit
Caulobacter segnis15250Cseg_2353protocatechuate 3,4-dioxygenase, alpha subunit
Corynebacterium ammoniagenes12612HMPREF0281_02557protocatechuate 3,4-dioxygenase, alpha subunit
Corynebacterium callunae15359H924_10090protocatechuate 3,4-dioxygenase alpha chain
Corynebacterium efficiens100395CE2300putative protocatechuate 3,4-dioxygenase alpha chain
Corynebacterium glutamicum12168Cgl2397Protocatechuate 3,4-dioxygenase beta subunit
Corynebacterium glutamicum12153KIQ_00515protocatechuate 3,4-dioxygenase, alpha subunit
Cupriavidus metallidurans102507Rmet_4014Protocatechuate (dihydroxy benzoate) 3,4-dioxygenase, alpha subunit
Enterobacter aerogenes13534EAE_20290protocatechuate 3,4-dioxygenase, alpha subunit
Escherichia fergusonii102419EFER_1501Protocatechuate 3,4-dioxygenase alpha chain
Geodermatophilus obscurus13315Gobs_2187protocatechuate 3,4-dioxygenase, alpha subunit
Gordonia aichiensis108223GOACH_11_00890protocatechuate 3,4-dioxygenase alpha subunit
Gordonia amarae15530GOAMR_52_00020protocatechuate 3,4-dioxygenase alpha subunit
Gordonia namibiensis108229GONAM_20_00380protocatechuate 3,4-dioxygenase alpha subunit
Gordonia otitidis100426GOOTI_112_00170protocatechuate 3,4-dioxygenase alpha subunit
Gordonia polyisoprenivorans16320GOPIP_038_00270protocatechuate 3,4-dioxygenase alpha subunit
Gordonia rhizosphera16068GORHZ_185_00180protocatechuate 3,4-dioxygenase alpha subunit
Gordonia sihwensis108236GSI01S_51_00050protocatechuate 3,4-dioxygenase alpha subunit
Gordonia sputi100414GOSPT_056_00130protocatechuate 3,4-dioxygenase alpha subunit
Halomonas elongata15536HELO_3955protocatechuate 3,4-dioxygenase, alpha subunit
Kineosphaera limosa100340KILIM_018_00060protocatechuate 3,4-dioxygenase alpha subunit
Klebsiella pneumoniae14438KPR_2986
Klebsiella pneumoniae subsp. rhinoscleromatis105682HMPREF0484_4663protocatechuate 3,4-dioxygenase, alpha subunit
Kocuria rhizophila103217KRH_06050protocatechuate 3,4-dioxygenase alpha subunit
Labrenzia aggregata16684SIAM614_01816protocatechuate 3,4-dioxygenase alpha subunit
Meiothermus ruber106122Mrub_2694protocatechuate 3,4-dioxygenase, alpha subunit
Nitriliruptor alkaliphilusNitalDRAFT_4767protocatechuate 3,4-dioxygenase alpha subunit
Ochrobactrum anthropi15819Oant_3728protocatechuate 3,4-dioxygenase, alpha subunit
Ochrobactrum intermedium15820OINT_2000814protocatechuate 3,4-dioxygenase, alpha subunit
Phaeobacter gallaeciensis16654PGA1_c02550protocatechuate 3,4-dioxygenase alpha chain
Pseudomonas putida100650PP_4655protocatechuate 3,4-dioxygenase, alpha subunit
Pseudomonas stutzeri14165PSTAB_1159protocatechuate 3,4-dioxygenase, alpha subunit
Rhizobium etli15573RHE_PE00056protocatechuate 3,4-dioxygenase alpha chain protein
Rhizobium sp.101917NGR_b21210protocatechuate 3,4-dioxygenase alpha chain
Rhizobium tropici15247RTCIAT899_PC07370protocatechuate 3,4-dioxygenase, alpha subunit
Rhodococcus erythropolis100887RER_52870protocatechuate 3,4-dioxygenase alpha subunit
Rhodococcus opacus108011ROP_10510protocatechuate 3,4-dioxygenase beta subunit
ROP_10520protocatechuate 3,4-dioxygenase alpha subunit
Roseobacter denitrificans15277RD1_3775protocatechuate 3,4-dioxygenase, alpha subunit
Roseobacter litoralis15278RLO149_c006690protocatechuate 3,4-dioxygenase alpha chain
Rubrobacter xylanophilus100952Rxyl_1580protocatechuate 3,4-dioxygenase, alpha subunit
Saccharomonospora azurea14651SacazDRAFT_04312protocatechuate 3,4-dioxygenase, alpha subunit
Saccharomonospora cyanea14841SaccyDRAFT_4392protocatechuate 3,4-dioxygenase, alpha subunit
Saccharomonospora viridis12207Svir_13020protocatechuate 3,4-dioxygenase, alpha subunit
Saccharopolyspora erythraea13426SACE_3513protocatechuate 3,4-dioxygenase alpha chain
Saccharopolyspora spinosa15153SspiN1_010100004763protocatechuate 3,4-dioxygenase subunit alpha
Saccharothrix espanaensis15066BN6_61200Protocatechuate 3,4-dioxygenase, alpha chain
Sphingomonas wittichii105917Swit_1072protocatechuate 3,4-dioxygenase, alpha subunit
Stackebrandtia nassauensis102104Snas_2325protocatechuate 3,4-dioxygenase, alpha subunit
Streptomyces avermitilis14893SAV1704putative protocatechuate 3,4-dioxygenase alpha subunit
Streptomyces ghanaensis15414SSFG_00971protocatechuate 3,4-dioxygenase alpha subunit
Streptomyces mobaraensis13819H340_23016protocatechuate 3,4-dioxygenase subunit alpha
Streptomyces sviceus13980SSEG_08942protocatechuate 3,4-dioxygenase, alpha subunit
Thermobispora bispora14880Tbis_2834protocatechuate 3,4-dioxygenase, alpha subunit
Thermomonospora curvata15933Tcur_3346protocatechuate 3,4-dioxygenase, alpha subunit
Tsukamurella paurometabola16120Tpau_0616protocatechuate 3,4-dioxygenase, alpha subunit