Rule : 4-hydroxy-2-oxovalerate aldolase (HOA)

Rule Description

NRULE_0064

Rule name

HOA

Created

2012-09-26

Protein name

Full: 4-hydroxy-2-oxovalerate aldolase
Short: HOA
EC: EC 4.1.3.39

Synonym

Full: 4-hydroxy-2-keto-pentanoic acid aldolase
4-hydroxy-2-oxopentanoate aldolase

Gene name

Not assigned

Rule Trigger

HAMAP; MF_01656; hit=1

Cross Reference

Gene Ontology

GO: GO:0019439, P:aromatic compound catabolic process; GO:0008701, F:4-hydroxy-2-oxovalerate aldolase activity; GO:0030145, F:manganese ion binding

Family and domain databases

HAMAP: MF_01656, HOA, 1, -
InterPro: IPR017629, 4OH_2_O-val_aldolase; IPR012425, DmpG_comm; IPR000891, PYR_CT
Pfam: PF07836, DmpG_comm, 1; PF00682, HMGL-like, 1
ProDom: PD005364, DmpG_comm, 1
TIGRFAMs: TIGR03217, 4OH_2_O_val_ald, 1
PROSITE: PS50991, PYR_CT, 1

Feature

P51016 Pseudomonas sp. (strain CF600).

ACT_SITE	21	Proton acceptor (Potential).
METAL	18	Manganese.
METAL	200	Manganese; via tele nitrogen.
METAL	202	Manganese; via tele nitrogen.
BINDING	171	Substrate.
BINDING	200	Substrate.
BINDING	291	Substrate.
SITE	17	Transition state stabilizer (By similarity).

General annotation

Function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds (By similarity)
Catalytic activity: 4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate
Sequence similarities: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Reference

P51020 Escherichia coli (strain K12).

9758851: Substrate selectivity and biochemical properties of 4-hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli. (Appl Environ Microbiol. 1998)

P51016 Pseudomonas sp. (strain CF600).

1732207: Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600. (J Bacteriol. 1992)

Related Function

Function name
Dihydroxy aromatic compounds degradation (via meta-cleavage pathway)

CDS


Scientific name	NBRC No.	CDS	Product name
Acinetobacter radioresistens	102413	ACRAD_06_00620	4-hydroxy-2-oxovalerate aldolase
Actinomadura atramentaria	14695	G339_12330	4-hyroxy-2-oxovalerate aldolase
Actinoplanes missouriensis	102363	AMIS_19100	putative 4-hydroxy-2-ketovalerate aldolase
Actinopolyspora iraqiensis	103187	ACTIR_570_00020	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase
Alicyclobacillus acidocaldarius subsp. acidocaldarius	15652	Aaci_1619	4-hydroxy-2-oxovalerate aldolase
Amycolatopsis azurea	14573	C791_1558	4-hydroxy-2-oxovalerate aldolase
Amycolatopsis mediterranei	13415	RAM_21495	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase
Bacillus pseudomycoides	101232	bpmyx0001_18650	4-hydroxy-2-oxovalerate aldolase
Brevibacillus brevis	100599	BBR47_29780	probable 4-hydroxy-2-oxovalerate aldolase
Caldilinea aerophila	104270	CLDAP_40960	putative 4-hydroxy-2-oxovalerate aldolase
Conexibacter woesei	100937	Cwoe_0441	4-hydroxy-2-oxovalerate aldolase
		Cwoe_4743	4-hydroxy-2-oxovalerate aldolase
		Cwoe_4833	4-hydroxy-2-oxovalerate aldolase
Corynebacterium ammoniagenes	12612	HMPREF0281_01858	4-hydroxy-2-oxovalerate aldolase
Cupriavidus metallidurans	102507	Rmet_1319	4-hydroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase, class I 4-Hydroxy-2-oxovalerate aldolase (protein belongs to CMGI-2)
Cupriavidus metallidurans	102507	Rmet_5209	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase, class I
Escherichia coli	3972	EcolC_3273	4-hydroxy-2-oxovalerate aldolase
Escherichia coli	13500	WFL_02115	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase
Escherichia coli	12713	Y75_0342	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase, class I
Gordonia aichiensis	108223	GOACH_04_04130	putative 4-hydroxy-2-oxovalerate aldolase
		GOACH_25_00010	putative 4-hydroxy-2-oxovalerate aldolase
		GOACH_43_00040	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia alkanivorans	16433	GOALK_026_00900	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia alkanivorans	16433	GOALK_050_01560	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia amarae	15530	GOAMR_19_00720	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia amarae	15530	GOAMR_41_00040	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia amicalis	100051	GOAMI_14_01060	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia araii	100433	GOARA_040_00140	4-hydroxy-2-oxovalerate aldolase
Gordonia bronchialis	16047	Gbro_0238	4-hydroxy-2-oxovalerate aldolase
Gordonia bronchialis	16047	Gbro_0911	4-hydroxy-2-oxovalerate aldolase
Gordonia effusa	100432	GOEFS_068_00110	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia hirsuta	16056	GOHSU_02_01030	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia namibiensis	108229	GONAM_07_00500	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia otitidis	100426	GOOTI_167_00010	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia polyisoprenivorans	16320	GOPIP_039_01150	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia polyisoprenivorans	16320	GOPIP_094_00890	4-hydroxy-2-oxovalerate aldolase CmtG
Gordonia rhizosphera	16068	GORHZ_115_00470	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia rhizosphera	16068	GORHZ_176_00090	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia rubripertincta	101908	GORBP_012_01420	putative 4-hydroxy-2-oxovalerate aldolase
		GORBP_032_00100	putative 4-hydroxy-2-oxovalerate aldolase
		GORBP_065_01330	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia sihwensis	108236	GSI01S_07_00500	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia soli	108243	GS4_15_01210	4-hydroxy-2-oxovalerate aldolase
Gordonia soli	108243	GS4_34_00020	4-hydroxy-2-oxovalerate aldolase
Gordonia sputi	100414	GOSPT_022_02640	putative 4-hydroxy-2-oxovalerate aldolase
		GOSPT_045_00280	putative 4-hydroxy-2-oxovalerate aldolase
		GOSPT_096_00220	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia terrae	100016	GOTRE_125_00700	putative 4-hydroxy-2-oxovalerate aldolase
Gordonia terrae	100016	GOTRE_150_00050	putative 4-hydroxy-2-oxovalerate aldolase
Intrasporangium calvum	12989	Intca_1094	4-hydroxy-2-oxovalerate aldolase
Kitasatospora setae	14216	KSE_23710	putative 4-hydroxy-2-oxovalerate aldolase
Klebsiella pneumoniae	14438	KPR_2662
Klebsiella pneumoniae subsp. rhinoscleromatis	105682	HMPREF0484_4291	4-hydroxy-2-oxovalerate aldolase
Kyrpidia tusciae	15312	Btus_0969	4-hydroxy-2-oxovalerate aldolase
Lentisphaera araneosa		LNTAR_03159	4-hydroxy-2-ketovalerate aldolase
Limnochorda pilosa	110152	LIP_2173	4-hydroxy-2-oxovalerate aldolase
Micromonospora aurantiaca	16155	Micau_4938	4-hydroxy-2-oxovalerate aldolase
Nakamurella multipartita	105858	Namu_4081	4-hydroxy-2-oxovalerate aldolase
Nitriliruptor alkaliphilus		NitalDRAFT_4813	4-hydroxy 2-oxovalerate aldolase
Rhodococcus erythropolis	100887	RER_07410	4-hydroxy-2-oxovalerate aldolase
Rhodococcus erythropolis	100887	RER_57580	probable 4-hydroxy-2-oxovalerate aldolase
Rhodococcus opacus	108011	ROP_34160	4-hydroxy-2-oxovalerate aldolase CmtG
		ROP_37360	4-hydroxy-2-oxovalerate aldolase
		ROP_44500	4-hydroxy-2-oxovalerate aldolase
		ROP_54050	4-hydroxy-2-oxovalerate aldolase
		ROP_58620	4-hydroxy-2-oxovalerate aldolase
		ROP_pROB02-02240	4-hydroxy-2-oxovalerate aldolase
Saccharomonospora glauca	14831	SacglDRAFT_02377	4-hydroxy-2-oxovalerate aldolase
Salinispora arenicola	105043	Q5S_2535_00010	4-hydroxy-2-ketovalerate aldolase
Salinispora tropica	105044	Strop_3525	pyruvate carboxyltransferase
Shimwellia blattae	105725	EB105725_09_00150	4-hydroxy-2-oxovalerate aldolase
Sphingobium japonicum	101211	SJA_C1-11910	4-hydroxy 2-oxovalerate aldolase
Sphingomonas wittichii	105917	Swit_1641	pyruvate carboxyltransferase
		Swit_2113	pyruvate carboxyltransferase
		Swit_4923	pyruvate carboxyltransferase
Sporichthya polymorpha	12702	Spopo_30_02770	4-hydroxy-2-oxovalerate aldolase
Streptomyces ghanaensis	15414	SSFG_07552	4-hydroxy-2-ketovalerate aldolase
Streptomyces ghanaensis	15414	SSFG_07759	pyruvate carboxyltransferase
Streptosporangium roseum	3776	Sros_9113	4-hydroxy-2-ketovalerate aldolase
Thauera linaloolentis	102519	C666_03895	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase
Thermomicrobium roseum		trd_1392	4-hydroxy-2-oxovalerate aldolase
Thermomonospora curvata	15933	Tcur_0536	4-hydroxy-2-oxovalerate aldolase
Thermomonospora curvata	15933	Tcur_3470	4-hydroxy-2-oxovalerate aldolase
Thermus thermophilus	101084	TTHB246	4-hydroxy-2-ketovalerate aldolase
Tsukamurella paurometabola	16120	Tpau_3800	4-hydroxy-2-oxovalerate aldolase