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Rule : Arsenate reductase (ARSC2)

Rule Description

ID NRULE_0018
Rule name ARSC2
Created 2012-07-10
Protein name
Protein name
Full
Arsenate reductase
EC
EC 1.20.4.-
Gene name arsC
Rule Trigger HAMAP; MF_01624; hit=1
OR
TIGRFAMs; TIGR02691; hit=1
OR
BLASTP; O68022; hit=1; score=260; pid=37
Cross Reference
Gene Ontology
GO
GO:0030612, F:arsenate reductase (thioredoxin) activity, IEA:InterPro
GO:0004725, F:protein tyrosine phosphatase activity, IEA:EC
GO:0035335, P:peptidyl-tyrosine dephosphorylation, IEA:GOC
GO:0046685, P:response to arsenic-containing substance, IEA:UniProtKB-KW
Phylogenomic databases
eggNOG
COG0394, -
HOGENOM
HOG000273093, -
KO
K03741, -
Family and domain databases
HAMAP
MF_01624, Arsenate_reduct, 1, -
InterPro
IPR014064, Arsenate_reductase_ArsC
IPR023485, Ptyr_pPase_SF
IPR017867, Tyr_phospatase_low_mol_wt
Pfam
PF01451, LMWPc, 1
SMART
SM00226, LMWPc, 1
TIGRFAMs
TIGR02691, ArsC_pI258_fam, 1
Feature
P0A006 Staphylococcus aureus.
ACT_SITE10Nucleophile; for reductase activity and phosphatase activity.
ACT_SITE82Nucleophile; for reductase activity.
ACT_SITE89Nucleophile; for reductase activity.
DISULFID10 - 82Redox-active; alternate.
DISULFID82 - 89Redox-active; alternate.
General annotation
Function
Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances
Catalytic activity
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H(2)O
Subunit structure
Monomer
Sequence similarities
Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family
Reference
P0A006 Staphylococcus aureus.
1409657
Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus plasmid pI258. (Proc Natl Acad Sci U S A. 1992)
10606519
The essential catalytic redox couple in arsenate reductase from Staphylococcus aureus. (Biochemistry. 1999)
P45947 Bacillus subtilis (strain 168).
9537360
The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite. (J Bacteriol. 1998)
O68022 Pseudomonas aeruginosa.
9802012
A chromosomal ars operon homologue of Pseudomonas aeruginosa confers increased resistance to arsenic and antimony in Escherichia coli. (Microbiology. 1998)



Related Function

Function name
Arsenical resistance

CDS

Data Download
Scientific name NBRC No. CDS Product name
Acetobacter aceti14818AaceN1_010100015122arsenate reductase
Acidiphilium multivorum100883ACMV_26670arsenate reductase
ACMV_P2_00140arsenate reductase
Acidithiobacillus ferrooxidansLferr_2481protein tyrosine phosphatase
Acidobacterium capsulatum15755ACP_3442arsenate reductase
Acinetobacter radioresistens102413ACRAD_60_00170arsenate reductase
Acinetobacter sp.100985ACT4_006_00500putative arsenate reductase
Alicyclobacillus acidocaldarius subsp. acidocaldarius15652Aaci_0537arsenate reductase (thioredoxin)
Aaci_2033arsenate reductase (thioredoxin)
Alteromonas macleodii102226MASE_14595arsenate reductase
Anaerolinea thermophila100420ANT_00450arsenate reductase
Anoxybacillus flavithermus15317Aflv_1414Protein phosphatase, low molecular weight phosphatase family
Ardenticatena maritimaARMA_2728arsenate reductase
Azorhizobium caulinodans14845AZC_1270low molecular weight phosphotyrosine protein phosphatase
Bacillus atrophaeus13721BATRMS_04_00260arsenate reductase
Bacillus cereus15305BC_3152Arsenate reductase
Bacillus licheniformis12200BL01871arsenate reductase
Bacillus licheniformis12195BLi02122putative arsenate reductase ArsC
Bacillus mojavensis15718GYU_03_03670arsenate reductase
Bacillus mycoides101228bmyco0001_28310hypothetical protein
Bacillus pseudomycoides101232bpmyx0001_14890hypothetical protein
Bacillus psychrosaccharolyticus101233WYK_198_00160arsenate reductase
Bacillus subtilis3134BSU6633_17355arsenate reductase
Bacillus subtilis subsp. subtilis13719BSU25780thioredoxin-coupled arsenate reductase
Bacillus thuringiensis101235bthur0008_28930hypothetical protein
Bacillus vallismortis101236BvalD_010100019152arsenate reductase
Bradyrhizobium diazoefficiens14792bll3086putative arsenate reductase (EC 1.97.1.5)
Brevibacillus brevis100599BBR47_33340arsenate reductase
Caldanaerobacter subterraneus subsp. tengcongensis100824TTE1001Protein-tyrosine-phosphatase
Caulobacter segnis15250Cseg_2399Protein-tyrosine phosphatase, low molecular weight
Cseg_2461Protein-tyrosine phosphatase, low molecular weight
Chromobacterium violaceum12614CV_2439arsenate reductase
Congregibacter litoralis104960KT71_13794Protein-tyrosine-phosphatase
Cupriavidus metallidurans102507Rmet_0331arsenate reductase (thioredoxin family)
Edwardsiella tarda105688ET1_07_00240putative arsenate reductase
Enterobacter cloacae subsp. cloacae13535ECL_00464hypothetical protein
ECL_00469arsenate reductase
ECL_03833arsenate reductase
Halobacillus halophilus102448HBHAL_4222arsenate reductase
Hylemonella gracilis14920HGR_08029protein tyrosine phosphatase
Kyrpidia tusciae15312Btus_1986arsenate reductase (thioredoxin)
Labrenzia aggregata16684SIAM614_25167protein tyrosine phosphatase
Lactobacillus casei15883LCABL_29620Arsenate reductase
Lactobacillus fermentum3956LAF_1155arsenate reductase
Lactobacillus hilgardii15886HMPREF0519_2696putative arsenate reductase (thioredoxin)
Lactobacillus plantarum subsp. plantarum15891HMPREF0531_12284arsenate reductase (thioredoxin)
Leuconostoc mesenteroides subsp. mesenteroides100496LEUM_A11Protein-tyrosine-phosphatase
Leuconostoc pseudomesenteroides107767LpseK3_010100001377arsenate reductase
LpseK3_010100021602arsenate reductase
Magnetospirillum gryphiswaldense15271MGR_2155low molecular weight protein-tyrosine-phosphatase
Magnetospirillum magnetotacticum15272Magn03009431COG0394: Protein-tyrosine-phosphatase
Magn03009432COG0394: Protein-tyrosine-phosphatase
Magn03009511COG0394: Protein-tyrosine-phosphatase
Magn03009512COG0394: Protein-tyrosine-phosphatase
Methylacidiphilum infernorumMinf_1582Arsenate reductase
Ochrobactrum anthropi15819Oant_3200protein tyrosine phosphatase
Paenibacillus alvei3343PAV_9c00330arsenate reductase (thioredoxin)
Paenibacillus polymyxa15309PPT_23700arsenate reductase
Phaeobacter gallaeciensis16654PGA1_c14990putative protein ArsC
PGA1_c15000putative transcriptional regulator
Pseudomonas putida100650PP_1928arsenate reductase
PP_2716arsenate reductase
Rhizobium etli15573RHE_CH02532putative protein-tyrosine-phosphatase protein
Rhizobium sp.101917NGR_b21810arsenate reductase
Rhodobacter capsulatus16581RCAP_rcc02070transcriptional regulator, ArsR family/protein tyrosine phosphatase
RCAP_rcp00087transcriptional regulator, ArsR family/protein tyrosine phosphatase
Rhodobacter sphaeroides12203RSP_4210transcriptional regulator, ArsR family
Roseobacter denitrificans15277RD1_2397arsenate reductase, putative
Roseobacter litoralis15278RLO149_c016860putative phosphotyrosine protein phosphatase
Sphingobium japonicum101211SJA_C1-04240arsenate reductase
Sphingobium sp.103272SLG_p_01440putative arsenate reductase
Sphingomonas echinoides15742SechA1_010100009993ArsR family transcriptional regulator
Staphylococcus epidermidis12993SE_0134arsenate reductase
Staphylococcus saprophyticus subsp. saprophyticus102446SSP1633arsenate reductase
SSPP114arsenate reductase
Starkeya novella14993Snov_1280transcriptional regulator, ArsR family
Snov_1590Protein-tyrosine phosphatase, low molecular weight
Thauera linaloolentis102519C666_06950arsenate reductase