Rule : Beta-ketoadipyl-CoA thiolase (PCAF)

Rule Description

NRULE_0074

Rule name

PCAF

Created

2012-10-23

Protein name

Full: Beta-ketoadipyl-CoA thiolase
EC: EC 2.3.1.174

Synonym

Full: 3-oxoadipyl-CoA thiolase

Gene name

pcaF, catF, paaJ

Rule Trigger

TIGRFAMs; TIGR02430; hit=1

BLASTP; Q0SH22; hit=1; score=1070; pid=58

BLASTP; Q0SCR8; hit=1; score=1050; pid=50

BLASTP; Q9XD81; hit=1; score=1010; pid=50

Cross Reference

Gene Ontology

GO: GO:0033812, F:3-oxoadipyl-CoA thiolase activity, IEA:EC; GO:0042952, P:beta-ketoadipate pathway, IEA:UniProtKB-UniPathway; GO:0019619, P:protocatechuate catabolic process, IEA:InterPro

Phylogenomic databases

KO: K07823, -

Family and domain databases

InterPro: IPR012793, PcaF; IPR002155, Thiolase; IPR016039, Thiolase-like; IPR016038, Thiolase-like_subgr; IPR020615, Thiolase_acyl_enz_int_AS; IPR020610, Thiolase_AS; IPR020617, Thiolase_C; IPR020613, Thiolase_CS; IPR020616, Thiolase_N
Pfam: PF02803, Thiolase_C, 1; PF00108, Thiolase_N, 1
PIRSF: PIRSF000429, Ac-CoA_Ac_transf, 1
TIGRFAMs: TIGR01930, AcCoA-C-Actrans, 1; TIGR02430, pcaF, 1
PROSITE: PS00098, THIOLASE_1, 1; PS00737, THIOLASE_2, 1; PS00099, THIOLASE_3, 1

Feature

P0C7L2 Escherichia coli (strain K12).

ACT_SITE	90	Acyl-thioester intermediate.
ACT_SITE	357	Proton acceptor.
ACT_SITE	387	Proton acceptor.

General annotation

Function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA
Catalytic activity: Succinyl-CoA + acetyl-CoA = CoA + 3-oxoadipyl- CoA
Pathway: Aromatic compound metabolism; beta-ketoadipate pathway; acetyl-CoA and succinyl-CoA from 3-oxoadipate: step 2/2
Aromatic compound metabolism; phenylacetate degradation
Sequence similarities: Belongs to the thiolase family

Reference

P0C7L2 Escherichia coli (strain K12).

17259607: Characterization of the last step of the aerobic phenylacetic acid degradation pathway. (Microbiology. 2007)

Q8VPF1 Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13).

11741863: Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. (J Bacteriol. 2002)

Q0SH22 Rhodococcus jostii (strain RHA1).

15937168: Catabolism of benzoate and phthalate in Rhodococcus sp. strain RHA1: redundancies and convergence. (J Bacteriol. 2005)

Q0SCR8 Rhodococcus jostii (strain RHA1).

15968060: Phenylacetate catabolism in Rhodococcus sp. strain RHA1: a central pathway for degradation of aromatic compounds. (J Bacteriol. 2005)

Q9XD81 Streptomyces sp. 2065.

10742233: Characterization of the protocatechuic acid catabolic gene cluster from Streptomyces sp. strain 2065. (Appl Environ Microbiol. 2000)

Related Function

Function name
Catechol degradation (beta-ketoadipate pathway)
Chlorocatechol degradation (beta-ketoadipate pathway)
Protocatechuate degradation (beta-ketoadipate pathway)

CDS


Scientific name	NBRC No.	CDS	Product name
Acaricomes phytoseiuli	107788	C501_07870	hypothetical protein
Acetobacter tropicalis	101654	ATPR_0361	beta-ketoadipyl CoA thiolase
Acidiphilium multivorum	100883	ACMV_20880	beta-ketoadipyl-CoA thiolase
Acinetobacter radioresistens	102413	ACRAD_20_00090	beta-ketoadipyl-CoA thiolase PcaF
Acinetobacter sp.	100985	ACT4_036_01090	beta-ketoadipyl-CoA thiolase
Acinetobacter sp.	100985	ACT4_038_00390	beta-ketoadipyl-CoA thiolase
Actinomadura atramentaria	14695	G339_12505	acetyl-CoA acetyltransferase
Actinoplanes missouriensis	102363	AMIS_42650	putative acyl-CoA thiolase
Actinosynnema mirum	14064	Amir_0164	acetyl-CoA acetyltransferase
Alicyclobacillus acidocaldarius subsp. acidocaldarius	15652	Aaci_0816	acetyl-CoA acetyltransferase
Amycolatopsis azurea	14573	C791_0884	3-ketoacyl-CoA thiolase
Amycolatopsis mediterranei	13415	RAM_09435	acetyl-CoA acyltransferase
Ardenticatena maritima		ARMA_0725	hypothetical protein
Arthrobacter globiformis	12137	ARGLB_008_00520	putative beta-ketoadipyl-CoA thiolase PaaJ
Arthrobacter globiformis	12137	ARGLB_036_00440	putative beta-ketoadipyl-CoA thiolase
Austwickia chelonae	105200	AUCHE_22_00410	putative beta-ketoadipyl-CoA thiolase
Azorhizobium caulinodans	14845	AZC_0996	beta-ketoadipyl CoA thiolase
Balneola vulgaris		B155_RS0109355	3-oxoadipyl-CoA thiolase
Bergeyella zoohelcum	16014	HMPREF9699_01792	3-oxoadipyl-CoA thiolase
Bradyrhizobium diazoefficiens	14792	blr0925	beta-ketoadipyl CoA thiolase
Brevibacterium linens	12170	BlinB_010200003739	putative beta-ketoadipyl CoA thiolase
Brevibacterium linens	12170	BlinB_010200004389	acetyl-CoA acetyltransferase
Candidatus Solibacter usitatus		Acid_0251	beta-ketoadipyl CoA thiolase
Catenulispora acidiphila	102108	Caci_6183	beta-ketoadipyl CoA thiolase
Caulobacter segnis	15250	Cseg_1757	beta-ketoadipyl CoA thiolase
Chromobacterium violaceum	12614	CV_4291	Beta-ketoadipyl CoA thiolase
Chryseobacterium gleum	15054	HMPREF0204_10052	beta-ketoadipyl CoA thiolase
Conexibacter woesei	100937	Cwoe_2156	acetyl-CoA acetyltransferase
Conexibacter woesei	100937	Cwoe_2196	beta-ketoadipyl CoA thiolase
Corynebacterium ammoniagenes	12612	HMPREF0281_01021	putative beta-ketoadipyl CoA thiolase
Corynebacterium ammoniagenes	12612	HMPREF0281_01033	acetyl-CoA C-acetyltransferase
Corynebacterium callunae	15359	H924_10065	acetyl-CoA acetyltransferase
Corynebacterium efficiens	100395	CE0673	putative beta-ketoadipyl CoA thiolase
Corynebacterium efficiens	100395	CE2295	putative acetyl-CoA acetyltransferase
Corynebacterium glutamicum	12168	Cgl2392	Acetyl-CoA acetyltransferases
Corynebacterium glutamicum	12153	KIQ_00540	acetyl-CoA acetyltransferase
Corynebacterium striatum	15291	HMPREF0308_2551	putative beta-ketoadipyl CoA thiolase
Corynebacterium terpenotabidum	14764	A606_01835	hypothetical protein
Cupriavidus metallidurans	102507	Rmet_3669	acetyl-CoA acetyltransferase
Cystobacter fuscus	100088	D187_005848	3-ketoacyl-CoA thiolase
Enterobacter aerogenes	13534	EAE_18180	acetyl-CoA acetyltransferase
Enterobacter aerogenes	13534	EAE_20470	beta-ketoadipyl CoA thiolase
Enterobacter cloacae subsp. cloacae	13535	ECL_02144	beta-ketoadipyl CoA thiolase
Enterobacter hormaechei	105718	HMPREF9086_0303	monovalent cation/proton (H+) antiporter subunit D
Escherichia coli	3972	EcolC_2258	beta-ketoadipyl CoA thiolase
Escherichia coli	13500	WFL_07480	beta-ketoadipyl CoA thiolase
Escherichia coli	12713	Y75_1388	predicted beta-ketoadipyl CoA thiolase
Escherichia fergusonii	102419	EFER_1599	beta-ketoadipyl CoA thiolase
Escherichia fergusonii	102419	EFER_1634	Beta-ketoadipyl CoA thiolase
Fluviicola taffensis	106335	Fluta_2912	acetyl-CoA acetyltransferase
Gemmatimonas aurantiaca	100505	GAU_1060	putative beta-ketoadipyl-CoA thiolase
Geodermatophilus obscurus	13315	Gobs_3827	acetyl-CoA acetyltransferase
Gordonia aichiensis	108223	GOACH_04_04990	putative acetyl-CoA acetyltransferase
Gordonia aichiensis	108223	GOACH_07_01160	beta-ketoadipyl-CoA thiolase
Gordonia alkanivorans	16433	GOALK_038_00790	putative acetyl-CoA acetyltransferase
		GOALK_047_00190	beta-ketoadipyl-CoA thiolase
		GOALK_092_00090	putative acetyl-CoA acetyltransferase
Gordonia amarae	15530	GOAMR_23_00120	putative acetyl-CoA acetyltransferase
Gordonia amarae	15530	GOAMR_78_00290	beta-ketoadipyl-CoA thiolase
Gordonia amicalis	100051	GOAMI_26_00380	putative acetyl-CoA acetyltransferase
		GOAMI_40_00540	putative acetyl-CoA acetyltransferase
		GOAMI_53_00120	putative acetyl-CoA acetyltransferase
Gordonia araii	100433	GOARA_068_00030	acetyl-CoA acetyltransferase
Gordonia bronchialis	16047	Gbro_1524	acetyl-CoA acetyltransferase
Gordonia bronchialis	16047	Gbro_4166	acetyl-CoA acetyltransferase
Gordonia effusa	100432	GOEFS_092_00490	putative acetyl-CoA acetyltransferase
Gordonia hirsuta	16056	GOHSU_26_00060	putative acetyl-CoA acetyltransferase
Gordonia namibiensis	108229	GONAM_23_00530	putative acetyl-CoA acetyltransferase
		GONAM_25_00240	beta-ketoadipyl-CoA thiolase
		GONAM_61_00670	putative acetyl-CoA acetyltransferase
Gordonia otitidis	100426	GOOTI_123_00380	putative acetyl-CoA acyltransferase
Gordonia otitidis	100426	GOOTI_253_00020	putative acetyl-CoA acetyltransferase
Gordonia polyisoprenivorans	16320	GOPIP_010_00520	putative acetyl-CoA acetyltransferase
Gordonia polyisoprenivorans	16320	GOPIP_011_00690	beta-ketoadipyl-CoA thiolase
Gordonia rhizosphera	16068	GORHZ_113_00110	putative acetyl-CoA acetyltransferase
		GORHZ_193_00120	putative acetyl-CoA acetyltransferase
		GORHZ_209_00350	beta-ketoadipyl-CoA thiolase
Gordonia rubripertincta	101908	GORBP_060_00230	putative acetyl-CoA acetyltransferase
		GORBP_060_00700	beta-ketoadipyl-CoA thiolase
		GORBP_108_00300	beta-ketoadipyl-CoA thiolase
Gordonia sihwensis	108236	GSI01S_15_00280	putative acetyl-CoA acetyltransferase
Gordonia sihwensis	108236	GSI01S_28_00300	beta-ketoadipyl-CoA thiolase
Gordonia soli	108243	GS4_08_00050	beta-ketoadipyl-CoA thiolase
Gordonia sputi	100414	GOSPT_045_01110	putative acetyl-CoA acetyltransferase
Gordonia sputi	100414	GOSPT_141_00260	beta-ketoadipyl-CoA thiolase
Gordonia terrae	100016	GOTRE_125_00220	beta-ketoadipyl-CoA thiolase
Halomonas elongata	15536	HELO_3960	beta-ketoadipyl CoA thiolase
Hylemonella gracilis	14920	HGR_08769	beta-ketoadipyl CoA thiolase
Hyphomonas neptunium	14232	HNE_0959	beta-ketothiolase
Intrasporangium calvum	12989	Intca_0377	beta-ketoadipyl CoA thiolase
Kineosphaera limosa	100340	KILIM_018_00090	beta-ketoadipyl-CoA thiolase
Kitasatospora setae	14216	KSE_64490	putative 3-oxoadipyl-CoA thiolase
Klebsiella pneumoniae	14438	KPR_2770
Klebsiella pneumoniae	14438	KPR_2865
Klebsiella pneumoniae subsp. rhinoscleromatis	105682	HMPREF0484_3061	beta-ketoadipyl CoA thiolase
Klebsiella pneumoniae subsp. rhinoscleromatis	105682	HMPREF0484_4170	beta-ketoadipyl CoA thiolase
Kocuria rhizophila	103217	KRH_02300	putative beta-ketoadipyl-CoA thiolase
Kordia algicida	100336	KAOT1_20542	enoyl-CoA hydratase
Kribbella flavida	14399	Kfla_6850	beta-ketoadipyl CoA thiolase
Ktedonobacter racemifer		Krac_12473	beta-ketoadipyl CoA thiolase
Kyrpidia tusciae	15312	Btus_1243	beta-ketoadipyl CoA thiolase
Kytococcus sedentarius	15357	Ksed_23240	acetyl-CoA acetyltransferase
Labrenzia aggregata	16684	SIAM614_04875	acetyl-CoA acetyltransferase
Labrenzia aggregata	16684	SIAM614_11098	acetyl-CoA acetyltransferase
Magnetospirillum gryphiswaldense	15271	MGR_1906	beta-ketoadipyl CoA thiolase
Magnetospirillum magnetotacticum	15272	Magn03007820	COG0183: Acetyl-CoA acetyltransferase
Marinomonas mediterranea	103028	Marme_3635	beta-ketoadipyl CoA thiolase
Meiothermus ruber	106122	Mrub_1323	acetyl-CoA acetyltransferase
Meiothermus silvanus	106475	Mesil_2321	acetyl-CoA acetyltransferase
Micrococcus luteus	3333	Mlut_20220	beta-ketoadipyl CoA thiolase
Micromonospora aurantiaca	16155	Micau_1439	beta-ketoadipyl CoA thiolase
Nakamurella multipartita	105858	Namu_0875	beta-ketoadipyl CoA thiolase
Nitriliruptor alkaliphilus		NitalDRAFT_4772	acetyl-CoA C-acetyltransferase
Nocardiopsis dassonvillei	14626	Ndas_2485	acetyl-CoA acetyltransferase
Ochrobactrum anthropi	15819	Oant_3718	beta-ketoadipyl CoA thiolase
Ochrobactrum anthropi	15819	Oant_4020	beta-ketoadipyl CoA thiolase
Ochrobactrum intermedium	15820	OINT_2000804	beta-ketoadipyl CoA thiolase
Paenibacillus alvei	3343	PAV_4c02980	beta-ketoadipyl-CoA thiolase PcaF
Phaeobacter gallaeciensis	16654	PGA1_c04090	beta-ketoadipyl-CoA thiolase PaaJ
Providencia alcalifaciens	105687	PROVALCAL_02758	beta-ketoadipyl CoA thiolase
Pseudomonas putida	100650	PP_1377	beta-ketoadipyl CoA thiolase PcaF
Pseudomonas putida	100650	PP_3280	beta-ketoadipyl CoA thiolase PhaD
Pseudomonas stutzeri	14165	PSTAB_1164	beta-ketoadipyl CoA thiolase
Renibacterium salmoninarum	15589	RSal33209_2690	3-ketoacyl-CoA thiolase
Rhizobium etli	15573	RHE_PE00203	beta-ketothiolase protein
Rhizobium sp.	101917	NGR_b21430	beta-ketoadipyl CoA thiolase PcaF
Rhizobium tropici	15247	RTCIAT899_PC07430	beta-ketoadipyl CoA thiolase
Rhodobacter sphaeroides	12203	RSP_3536	Thiolase
Rhodococcus erythropolis	100887	RER_52080	putative acetyl-CoA acyltransferase
Rhodococcus erythropolis	100887	RER_52910	putative beta-ketoadipyl-CoA thiolase
Rhodococcus opacus	108011	ROP_10560	putative beta-ketoadipyl-CoA thiolase
		ROP_22440	acetyl-CoA acetyltransferase
		ROP_25860	beta-ketoadipyl-CoA thiolase
		ROP_27210	acetyl-CoA acetyltransferase
Rubrobacter xylanophilus	100952	Rxyl_1582	Acetyl-CoA C-acetyltransferase
Saccharomonospora azurea	14651	SacazDRAFT_04144	acetyl-CoA acetyltransferase
Saccharomonospora cyanea	14841	SaccyDRAFT_1536	acetyl-CoA acetyltransferase
Saccharomonospora glauca	14831	SacglDRAFT_01422	acetyl-CoA acetyltransferase
Saccharomonospora viridis	12207	Svir_13000	acetyl-CoA acetyltransferase
Saccharopolyspora erythraea	13426	SACE_4011	acetyl-CoA C-acetyltransferase
Saccharopolyspora spinosa	15153	SspiN1_010100028869	acetyl-CoA acetyltransferase
Saccharothrix espanaensis	15066	BN6_27580	Beta-ketoadipyl-CoA thiolase
Salinispora tropica	105044	Strop_1359	beta-ketoadipyl CoA thiolase
Serratia odorifera	102598	HMPREF0758_3090	beta-ketoadipyl CoA thiolase
Shimwellia blattae	105725	EB105725_19_00620	beta-ketoadipyl-CoA thiolase
Shimwellia blattae	105725	EB105725_23_00500	beta-ketoadipyl-CoA thiolase
Sphingobium sp.	103272	SLG_28040	beta-ketoadipyl-CoA thiolase
Sphingomonas wittichii	105917	Swit_0752	beta-ketoadipyl CoA thiolase
		Swit_4336	beta-ketoadipyl CoA thiolase
		Swit_4887	beta-ketoadipyl CoA thiolase
		Swit_5019	beta-ketoadipyl CoA thiolase
Sporichthya polymorpha	12702	Spopo_30_12460	Acetyl-CoA acetyltransferase
Streptomyces avermitilis	14893	SAV1604	putative beta-ketoadipyl-CoA thiolase
Streptomyces cattleya	14057	SCAT_0670	beta-ketoadipyl CoA thiolase
Streptomyces clavuligerus	13307	SCLAV_5504	beta-ketoadipyl-CoA thiolase
Streptomyces ghanaensis	15414	SSFG_00870	beta-ketoadipyl-CoA thiolase
		SSFG_01807	acetyl-CoA acetyltransferase
		SSFG_07165	beta-ketoadipyl-CoA thiolase
Streptomyces mobaraensis	13819	H340_23001	beta-ketoadipyl-CoA thiolase
Streptomyces pristinaespiralis	13074	SSDG_02060	acetyl-CoA acetyltransferase
Streptomyces purpureus	13925	STRPU_17_03110	3-ketoacyl-CoA thiolase @ Acetyl-CoA acetyltrans ferase
Streptomyces sviceus	13980	SSEG_03720	beta-ketoadipyl CoA thiolase
Streptomyces sviceus	13980	SSEG_08943	beta-ketoadipyl CoA thiolase
Streptomyces venezuelae	13096	SVEN_6882	3-ketoacyl-CoA thiolase @ Acetyl-CoA acetyltransferase
Streptosporangium roseum	3776	Sros_7930	3-oxoadipyl-CoA thiolase
Thauera linaloolentis	102519	C666_06605	beta-ketoadipyl CoA thiolase
Thermobispora bispora	14880	Tbis_1802	acetyl-CoA acetyltransferase
Thermomonospora curvata	15933	Tcur_1860	acetyl-CoA acetyltransferase
Thermus thermophilus	101084	TTHA0987	beta-ketoadipyl CoA thiolase
Thermus thermophilus	101085	TT_C0623	beta-ketoadipyl CoA thiolase
Tsukamurella paurometabola	16120	Tpau_0619	acetyl-CoA acetyltransferase
Turicella otitidis	106139	HMPREF9719_01826	3-oxoadipyl-CoA thiolase
Zimmermannella faecalis	15706	G328_00785	hypothetical protein