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Rule : 23S rRNA (guanine-N(1))-methyltransferase (RLMA)

Rule Description

ID NRULE_0165
Rule name RLMA
Created 2013-03-19
Protein name
Protein name
Full
23S rRNA (guanine-N(1))-methyltransferase
EC
EC 2.1.1.-
Synonym
Full
Ribosomal RNA large subunit methyltransferase A
Gene name rlmA
Rule Trigger PIRSF; PIRSF018249; hit=1
Cross Reference
Gene Ontology
GO
GO:0052911, F:23S rRNA (guanine(745)-N(1))-methyltransferase activity, IEA:EC
GO:0052912, F:23S rRNA (guanine(748)-N(1))-methyltransferase activity, IDA:UniProtKB
GO:0046872, F:metal ion binding, IEA:UniProtKB-KW
GO:0070475, P:rRNA base methylation, IDA:UniProtKB
Phylogenomic databases
KO
K00563, -
Family and domain databases
InterPro
IPR016718, rRNA_m1G-MeTrfase_A_prd
PIRSF
PIRSF018249, MyrA_prd, 1
Feature
P36999 Escherichia coli (strain K12).
METAL5Zinc.
METAL8Zinc.
METAL21Zinc.
METAL25Zinc.
BINDING67S-adenosyl-L-methionine.
BINDING183S-adenosyl-L-methionine.
General annotation
Function
Catalyzes N1-methylation of a guanine base (G745 in Gram-negative and G748 in Gram-positive bacteria) of 23S rRNA. Confers resistance to the macrolide antibiotics
Catalytic activity
S-adenosyl-L-methionine + guanine(745) in 23S rRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(745) in 23S rRNA
S-adenosyl-L-methionine + guanine(748) in 23S rRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(748) in 23S rRNA
Sequence similarities
Belongs to the methyltransferase superfamily. RlmA family
Reference
P36999 Escherichia coli (strain K12).
14999102
Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site. (Proc Natl Acad Sci U S A. 2004)
Q9S1M6 Streptomyces fradiae (Streptomyces roseoflavus).
10348045
Molecular analysis of tlrB, an antibiotic-resistance gene from tylosin-producing Streptomyces fradiae, and discovery of a novel resistance mechanism. (J Antibiot (Tokyo). 1999)



Related Function

Function name
Macrolide antibiotic resistance

CDS

Data Download
Scientific name NBRC No. CDS Product name
Acinetobacter radioresistens102413ACRAD_07_0046023S rRNA methyltransferase RrmA
Acinetobacter sp.100985ACT4_019_01490ribosomal RNA large subunit methyltransferase A
Actinoalloteichus cyanogriseus14455G443_01_02260ribosomal RNA methyltransferase
Actinoalloteichus spitiensis102582ACTIDRAF_10370hypothetical protein
Actinomadura atramentaria14695G339_07345hypothetical protein
Actinoplanes missouriensis102363AMIS_74790putative rRNA methyltransferase
Aerococcus viridans12219HMPREF0061_0657hypothetical protein
Alteromonas macleodii102226MASE_09260rRNA (guanine-N1-)-methyltransferase
Amycolatopsis azurea14573C791_1806Ribosomal RNA large subunit methyltransferase A
Arthrobacter globiformis12137ARGLB_058_00250putative methyltransferase
Austwickia chelonae105200AUCHE_05_04260putative rRNA methyltransferase
Bacillus amyloliquefaciens15535BAMF_3725rRNA (guanine-N1-)-methyltransferase, putative
Bacillus subtilis3134BSU6633_01834putative ribosomal RNA methyltransferase
Bacillus subtilis subsp. subtilis13719BSU39010putative ribosomal RNA methyltransferase
Brevibacillus brevis100599BBR47_42430putative rRNA methyltransferase
Catenulispora acidiphila102108Caci_1726Methyltransferase type 11
Clostridium kluyveri12016CKR_2309hypothetical protein
Congregibacter litoralis104960KT71_10964methyltransferase
Coprobacillus sp.HMPREF1021_00177hypothetical protein
Corynebacterium ammoniagenes12612HMPREF0281_01967methyltransferase domain protein
Corynebacterium callunae15359H924_05255hypothetical protein
Corynebacterium efficiens100395CE1172putative mycinamicin-resistance protein
Corynebacterium glutamicum12168Cgl1115SAM-dependent methyltransferases
Corynebacterium glutamicum12153KIQ_15622hypothetical protein
Corynebacterium matruchotii15360HMPREF0299_5466methyltransferase domain protein
Corynebacterium striatum15291HMPREF0308_0051methyltransferase domain protein
Corynebacterium terpenotabidum14764A606_07580ribosomal RNA methyltransferase
Edwardsiella tarda105688ET1_04_0055023S rRNA methyltransferase RlmA
Enterobacter aerogenes13534EAE_22630rRNA (guanine-N(1)-)-methyltransferase
Enterobacter cloacae subsp. cloacae13535ECL_0148223S rRNA methyltransferase A
Enterobacter hormaechei105718HMPREF9086_2767ribosomal RNA large subunit methyltransferase A
Enterococcus gilvus100696I592_00958methyltransferase
Enterococcus haemoperoxidus100709I583_01895ribosomal RNA large subunit methyltransferase A
Enterococcus hirae3181EHR_02905ribosomal RNA large subunit methyltransferase A
Enterococcus italicus107825HMPREF9088_0182methyltransferase domain protein
Enterococcus moraviensis100710I586_01686ribosomal RNA large subunit methyltransferase A
Enterococcus pallens100697I588_03074methyltransferase
Enterococcus phoeniculicola100711I589_02668methyltransferase
Escherichia coli3972EcolC_1810rRNA (guanine-N(1)-)-methyltransferase
Escherichia coli13500WFL_0978523S rRNA methyltransferase A
Escherichia coli12713Y75_181923S rRNA m1G745 methyltransferase
Escherichia fergusonii102419EFER_1253Ribosomal RNA large subunit methyltransferase A (rRNA (guanine-N(1)-)-methyltransferase) (23S rRNA m1G745 methyltransferase)
Escherichia hermannii105704EH105704_01_0314023S rRNA methyltransferase RlmA
Geodermatophilus obscurus13315Gobs_1348Methyltransferase type 11
Gordonia aichiensis108223GOACH_03_06130putative rRNA methyltransferase
Gordonia amarae15530GOAMR_69_00570putative methyltransferase
Gordonia amicalis100051GOAMI_15_01520putative methyltransferase
Gordonia araii100433GOARA_061_00680putative methyltransferase
Gordonia bronchialis16047Gbro_3307Methyltransferase type 11
Gordonia effusa100432GOEFS_132_00920putative methyltransferase
Gordonia hirsuta16056GOHSU_04_00860putative methyltransferase
Gordonia namibiensis108229GONAM_09_00110putative methyltransferase
Gordonia otitidis100426GOOTI_115_00080putative rRNA methyltransferase
Gordonia rhizosphera16068GORHZ_123_00160putative rRNA methyltransferase
Gordonia sihwensis108236GSI01S_10_00730putative methyltransferase
Gordonia soli108243GS4_02_01310putative methyltransferase
Gordonia terrae100016GOTRE_018_00270putative methyltransferase
Halomonas elongata15536HELO_1632rRNA (guanine-N(1)-)-methyltransferase
Kineosphaera limosa100340KILIM_011_00620putative methyltransferase
Kitasatospora setae14216KSE_26550putative methyltransferase
Klebsiella pneumoniae14438KPR_3250
Klebsiella pneumoniae subsp. rhinoscleromatis105682HMPREF0484_2134ribosomal RNA large subunit methyltransferase A
Ktedonobacter racemiferKrac_8787rRNA (guanine-N(1)-)-methyltransferase
Lactobacillus acidipiscis102164LaciK1_01010000945123S rRNA m(1)G 745 methyltransferase
Lactobacillus animalis15882LaniK3_01010000596723S rRNA m(1)G 745 methyltransferase
Lactobacillus casei15883LCABL_10260RRNA large subunit methyltransferase A
Lactobacillus farciminis107150LfarK3_010100001677rRNA (guanine-N1-) methyltransferase (mycinamicin-resistance protein)
Lactobacillus fermentum15885HMPREF0511_0954methyltransferase domain protein
Lactobacillus fermentum3956LAF_0564rRNA methyltransferase
Lactobacillus hilgardii15886HMPREF0519_1744methyltransferase domain protein
Lactobacillus plantarum subsp. plantarum15891HMPREF0531_12236methyltransferase domain protein
Lactobacillus reuteri15892LAR_0563rRNA large subunit methyltransferase A
Marinomonas mediterranea103028Marme_2493rRNA (guanine-N(1)-)-methyltransferase
Microlunatus phosphovorus101784MLP_14630putative methyltransferase
Micromonospora aurantiaca16155Micau_0781rRNA (guanine-N(1)-)-methyltransferase
Mobilicoccus pelagius104925MOPEL_021_00650putative methyltransferase
Nakamurella multipartita105858Namu_0717rRNA (guanine-N(1)-)-methyltransferase
Nitriliruptor alkaliphilusNitalDRAFT_491123S rRNA m(1)G-748 methyltransferase
Nocardiopsis dassonvillei14626Ndas_2268rRNA guanine-N1-methyltransferase
Oscillibacter valericigenes101213OBV_39930ribosomal RNA large subunit methyltransferase A
Paenibacillus polymyxa15309PPT_21645rRNA (guanine-N1)-methyltransferase
Photobacterium damselae subsp. damselae corrig.15633VDA_001468putative SAM-dependent methyltransferase
Propionibacterium acidipropionici12425PACID_22350Methyltransferase domain-containing protein
Propionibacterium avidum15671HMPREF9153_0163rRNA (guanine-N(1)-)-methyltransferase
Proteus mirabilis105697HMPREF0693_1237methyltransferase domain protein
Providencia alcalifaciens105687PROVALCAL_01859methyltransferase domain protein
Pseudomonas putida100650PP_1524rRNA large subunit methyltransferase A, putative
Pseudomonas stutzeri14165PSTAB_2676rRNA methyltransferase
Rahnella aquatilis105701Rahaq2_2927methylase involved in ubiquinone/menaquinone biosynthesis
Renibacterium salmoninarum15589RSal33209_201923S rRNA m(1)G 745 methyltransferase
Rhodococcus erythropolis100887RER_41620putative methyltransferase
Rhodococcus opacus108011ROP_60330putative rRNA methyltransferase
Saccharomonospora azurea14651SacazDRAFT_03819methylase involved in ubiquinone/menaquinone biosynthesis
Saccharomonospora cyanea14841SaccyDRAFT_1117hypothetical protein
Saccharomonospora viridis12207Svir_09700methyltransferase family protein
Saccharopolyspora erythraea13426SACE_1994rRNA guanine-N1-methyltransferase
Saccharopolyspora spinosa15153SspiN1_010100027544methyltransferase type 11
Salinispora tropica105044Strop_0814Methyltransferase type 11
Serratia odorifera102598HMPREF0758_3320methyltransferase domain protein
Shimwellia blattae105725EB105725_38_0035023S rRNA methyltransferase RlmA
Streptococcus mitis106071SM12261_0675rRNA (guanine-N1-)-methyltransferase
Streptomyces avermitilis14893SAV4467putative rRNA methyltransferase
Streptomyces cattleya14057SCAT_5649Mycinamicin-resistance protein myrA
Streptomyces ghanaensis15414SSFG_00294rRNA methyltransferase
Streptomyces mobaraensis13819H340_17722rRNA-guanine-N1-methyltransferase
Streptosporangium roseum3776Sros_0404rRNA (guanine-N(1)-)-methyltransferase
Tetragenococcus halophilus12172TEH_06860putative methyltransferase
Thauera linaloolentis102519C666_09795rRNA (guanine-N(1)-)-methyltransferase
Thermobispora bispora14880Tbis_0187Methyltransferase type 11
Thermomonospora curvata15933Tcur_2637rRNA (guanine-N(1)-)-methyltransferase
Tsukamurella paurometabola16120Tpau_1173Methyltransferase type 11
Turicella otitidis106139HMPREF9719_00969hypothetical protein
Vibrio alginolyticus15630N646_1267rRNA (guanine-N1-)-methyltransferase
Vibrio ichthyoenteri15847VII00023_1926423S rRNA methyltransferase A
Vibrio metschnikovii103153VIB_000865rRNA (guanine-N1-)-methyltransferase
Vibrio orientalis15638VIOR3934_1121223S rRNA methyltransferase A
Vibrio tubiashii15644VITU9109_0452223S rRNA methyltransferase A
Vibrio vulnificus15645B736_0458023S rRNA methyltransferase
Yersinia bercovieri105717yberc0001_20240rRNA (Guanine-N1-)-methyltransferase
Yersinia rohdei105715yrohd0001_29070rRNA (Guanine-N1-)-methyltransferase